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qingmo0729 committed Oct 8, 2023
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7 changes: 6 additions & 1 deletion asserts/css/style.css
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Expand Up @@ -416,7 +416,12 @@ body.dark-theme .translate-icon:hover path {
.navbar-toggler-icon:hover {
color: #114261;
}

.workflow {
width: 8rem;
height: auto;
text-align: center;
transform: translateY(-1rem);
}
/* 链接 hover下划线 */
a {
position: relative;
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20 changes: 10 additions & 10 deletions pages/design.html
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Expand Up @@ -484,23 +484,23 @@ <h2 id="06" class="module">The Work Flow</h2>
<p class="cn d-none">
我们的项目计划主要分为CRISPRi测试系统及菌毛表达系统的建立,CRISPRi的优化,金属还原地杆菌菌毛的表达与收获,电导率以及浓度检测能力的测试,以及菌毛的优化和检测能力的提高。下图简要总结了Plink项目的工作流程。
</p>
<p class="en"><strong>Establishment of CRISPRi testing system and pilus expression system:</strong> co-transfections of
<p class="en"><img src="../asserts/img/design/wf1.png" alt="Work Flow1" id="wf1" class="img-fluid workflow"><strong>Establishment of CRISPRi testing system and pilus expression system:</strong> co-transfections of
pdCas9-sg and pPilin, and of pdCas9-sg and the reporter plasmid, into Vibrio natriegens, respectively.
</p>
<p class="cn d-none"><strong>系统建立:</strong> CRISPRi测试系统及菌毛表达系统的建立,将pdCas9-sg和pPilin,pdCas9-sg和报告质粒分别共转入需钠弧菌中</p>
<p class="en"><strong>CRISPRi optimization:</strong> construct pdCas9-sg plasmids with different sgRNAs by gibson assembly to
<p class="cn d-none"> <img src="../asserts/img/design/wf1.png" alt="Work Flow1" id="wf1" class="img-fluid workflow"><strong>系统建立:</strong> CRISPRi测试系统及菌毛表达系统的建立,将pdCas9-sg和pPilin,pdCas9-sg和报告质粒分别共转入需钠弧菌中</p>
<p class="en"><img src="../asserts/img/design/wf2.png" alt="Work Flow2" id="wf2" class="img-fluid workflow"><strong>CRISPRi optimization:</strong> construct pdCas9-sg plasmids with different sgRNAs by gibson assembly to
test the sgRNA with optimal effect.</p>
<p class="cn d-none"><strong>CRISPRi优化:</strong>通过gibson assembly构建不同sgRNA的pdCas9-sg质粒,测试效果最优的sgRNA</p>
<p class="en"><strong>Expression and harvest of pilus:</strong> induce the expression of dCas9 and pilus in Geobacter
<p class="cn d-none"><img src="../asserts/img/design/wf2.png" alt="Work Flow2" id="wf2" class="img-fluid workflow"><strong>CRISPRi优化:</strong>通过gibson assembly构建不同sgRNA的pdCas9-sg质粒,测试效果最优的sgRNA</p>
<p class="en"><img src="../asserts/img/design/wf3.png" alt="Work Flow3" id="wf3" class="img-fluid workflow"><strong>Expression and harvest of pilus:</strong> induce the expression of dCas9 and pilus in Geobacter
metallireducens, and harvest the pilus.</p>
<p class="cn d-none"><strong>菌毛表达与收获:</strong>诱导dCas9与金属还原地杆菌的菌毛表达,并收获菌毛</p>
<p class="en"><strong>Conductivity and concentration detection capability test:</strong> construct pilus sheets and test
<p class="cn d-none"><img src="../asserts/img/design/wf3.png" alt="Work Flow3" id="wf3" class="img-fluid workflow"><strong>菌毛表达与收获:</strong>诱导dCas9与金属还原地杆菌的菌毛表达,并收获菌毛</p>
<p class="en"><img src="../asserts/img/design/wf4.png" alt="Work Flow4" id="wf4" class="img-fluid workflow"><strong>Conductivity and concentration detection capability test:</strong> construct pilus sheets and test
their conductivities at different pH, as well as their conductivities before and after the binding of
the analyte.</p>
<p class="cn d-none"><strong>测试:</strong>制作菌毛片,测试其在不同pH环境下,以及与待测物结合前后的电导率</p>
<p class="en"><strong>Pilus optimization: </strong>optimize the pili gene sequence based on modeling in order to enhance the
<p class="cn d-none"><img src="../asserts/img/design/wf4.png" alt="Work Flow4" id="wf4" class="img-fluid workflow"><strong>测试:</strong>制作菌毛片,测试其在不同pH环境下,以及与待测物结合前后的电导率</p>
<p class="en"><img src="../asserts/img/design/wf5.png" alt="Work Flow5" id="wf5" class="img-fluid workflow"><strong>Pilus optimization: </strong>optimize the pili gene sequence based on modeling in order to enhance the
conductivity and detection sensitivity of the pilus.</p>
<p class="cn d-none"><strong>菌毛优化:</strong>根据建模结果优化菌毛序列结构,提高其电导率和检测灵敏度</p>
<p class="cn d-none"><img src="../asserts/img/design/wf5.png" alt="Work Flow5" id="wf5" class="img-fluid workflow"><strong>菌毛优化:</strong>根据建模结果优化菌毛序列结构,提高其电导率和检测灵敏度</p>
<h2 id="07" class="module">Vector Construction</h2>
<p class="en">In our Plink project, three major plasmids were designed to achieve the expression and harvest
of high-purity Geobacter metallireducens conductive pilus in Vibrio natriegens. Among them, pPilin is
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