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-                                            On the other hand, we have also sought for equipping our method with capacity to meet the demand and solve the urgent problems in real industry, for which we had an on-site visit to Huangheng Biotechnology Co., Ltd. The company focus on industrial application of synthetic biology, especially the fermentation of natural products. After a deep conversation with the xxx, we recognized that in addition of the efficiency, we also need to take the stability of the enzymes we used into consideration when applying the strategy of directed evolution. Using long-term maintenance of the fermentation tank as an example, they told us the complex condition in the real industrial fermentation, which really inspired us that in future we can evolve our strain to facilitate it with a better resistance of temperature and pHunder the condition of fermentation.
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+                                            On the other hand, we have actively worked on enhancing our method to meet the demands and address urgent issues in real-world industries. To achieve this, we conducted an on-site visit to Huangheng Biotechnology Co., Ltd., a company that specializes in the industrial application of synthetic biology, particularly in the fermentation of natural products.
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+                                                During our visit and in-depth discussions with their Research and Development Engineer, we came to realize that, aside from focusing on efficiency, we also need to consider the stability of the enzymes we employ when implementing the directed evolution strategy. For instance, they illustrated the challenges of maintaining fermentation tanks in complex industrial fermentation processes. This eye-opening experience inspired us to consider the possibility of evolving our strains in the future to make them more resilient to temperature and pH variations during fermentation—a step towards improving our approach for real-world industrial applications
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                                             In addition, we have also actively promoted the communication and collaboration among researchers nationwide who work on directed evolution. Last year we had an offline meetup with the undergraduate researchers in the school of life science of Sun Yat-Sen University and had a fruitful talk about applying continuous directed evolution into the further improvement of the yield. During this gathering, we have engaged in a knowledge exchange concerning TADR and nyEvolvR, the directed evolution systems we used in the design and compared their strength and weakness.
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