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@youduorua
youduorua authored Oct 7, 2023
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32 changes: 14 additions & 18 deletions safety/safety.html
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</a>
<ul>
<li>
<a href="../project/introduction.html" style="font-size:17px">Introduction</a>
<a href="../project/introduction.html" style="font-size:17px">Background</a>
</li>

<li>
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<li>
<a href="../hp/collaboration.html"style="font-size:19px">
<i class="ti-heart"></i> Collaboration</a>
<i class="ti-heart"></i>Human Practice</a>
</li>

<li>
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<h1 style="color: #002752;" >Overview </h1>
</br>
<div class="stat-digit">
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In view of the important role of safety in the experimental process, we considered safety as a very important consideration in the experimental design process. Meanwhile, we continued to consider safety risks throughout the project, and further reviewed safety issues in the process of adjusting the plan. We also carry out experiments in strict accordance with the safety regulations of the laboratory and the national safety regulations.
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Taking into account the critical role that safety plays in the experimental process, we have made safety a top priority throughout the experimental phase. Concurrently, we have continuously assessed potential safety risks throughout the project and further evaluated safety issues as we refined our plans. Moreover, we have conducted experiments in strict adherence to both laboratory-specific safety regulations and national safety standards.
</div>


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</br>

<div class="stat-digit">
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The materials we use in the process of the project are strictly selected, harmless to the human body and the environment. Our project is basically carried out at very low risk, because no disease-causing bacteria or dangerous chemicals are used in the process. The actual products we use also meet the requirements of safety issues.<br><br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We use strains including Escherichia coli BL21, Escherichia coli DH5 alpha, W303a (W303-1 a) Chemically Competent Cell, Bacillus subtilis WB800N. They all belong to risk Group One, which is low risk to humans and the environment.<br><br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The materials utilized throughout our project undergo stringent selection to ensure their safety for both human health and the environment. We have taken great care to minimize risks by strictly avoiding the use of disease-causing bacteria or hazardous chemicals. The actual products employed in our project also meet stringent safety requirements.<br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;For instance, the strains used, including Escherichia coli Nissle 1917, Escherichia coli DH5α, Y. lipolytica PO1f, and Kluyveromyces marxianus YDU010, all fall under the low-risk Group One category. This classification signifies their minimal impact on humans and the environment.
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<h1 style="color: #002752;" >Laboratory</h1>
</br>
<div class="stat-digit">
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Before conducting experiments, all our experimenters have received systematic experimental safety training. For example, we must properly wear experimental gloves and safety goggles when doing experiments. In addition, all of us are familiar with the relevant safety regulations and adhere to them under the supervision of our consultants and PI.
We have complete disinfection, sterilization equipment and procedures. For example, we will turn on the UV lamp for 15 minutes before using the ultra-clean table every time, and use alcohol to disinfect gloves and related articles during operation.
The reagents and instruments we use will be correctly marked and placed in accordance with the regulations.
After the completion of the experiment, we will concentrate on sterilizing the chemical and biological waste to avoid potential risks in the laboratory
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Prior to conducting any experiments, our team members receive comprehensive safety training to ensure that they are equipped to work safely in the laboratory. We strictly adhere to relevant safety regulations and guidelines under the supervision of our consultants and PI. All experimenters diligently make use of proper protective equipment, such as laboratory gloves and safety goggles during experiments.
<br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We possess complete disinfection and sterilization equipment and follow strict procedures to maintain the safety of everyone involved. For instance, before utilizing the ultra-clean table, we switch on the UV lamp for 15 minutes to disinfect the surface. Additionally, we frequently disinfect our gloves and related articles using alcohol during operation. To ensure safe storage and handling, we correctly label and place the reagents and instruments used in accordance with applicable regulations.
<br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Once an experiment is complete, we prioritize the swift and thorough sterilization of both chemical and biological waste to mitigate potential risks in the laboratory.
</div><br>

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<h1 style="color: #002752;" >Guidelines</h1>
</br>
<div class="stat-digit">
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;All the experiments we performed were in accordance with the General Guidelines for Biosafety in Microbiology and Biomedical Laboratories (China CDC). In addition, we have established the safety regulations that our laboratory must follow. <br>These include, but are not limited to:<br>
<ol>
<li>Responsible person.</li>
<li>Fire safety.</li>
<li>Power safety.</li>
<li>Safety rules for hazardous chemical reagents, including purchase, storage, etc.</li>
<li>Proper disposal of chemical and biological wastes.</li>
<li>Biosafety.</li>
</ol>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We have conducted all of our experiments in strict accordance with the General Guidelines for Biosafety in Microbiology and Biomedical Laboratories (China CDC). Furthermore, we have established our laboratory safety regulations that must be followed at all times to ensure the well-being of our team members and maintain a safe working environment.
<br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;These regulations cover a variety of topics, including but not limited to, the identification of a responsible person for overall safety measures, guidelines for fire and power safety, and specific rules governing the handling of hazardous chemical reagents, from purchase to storage. Proper and appropriate disposal methods for both chemical and biological waste are strictly adhered to as dictated by relevant safety guidelines. Lastly, biosafety measures are ingrained into all aspects of our experimental design and implementation to mitigate any potential risks.
<br>
</div><br>
<center>
<img src="../src/sa-1.png"
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