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- Computationally guided -

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- evolutionary engineering -

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- cpuchina -
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- of ancestral glycosyltransferases -

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- 2024 -

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- glucoside -
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are a large class
of biologically
active natural
products.

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Among them, C-
glycosides
are stable
for enzymatic or
hydrolysis.

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C-Glycosides

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- antioxidant -
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The antioxidant
capacity
of flavonoid
glycosides produces
important health
promoting activities

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Flavonoid

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Such as anti-aging,
anti-cancer, anti-
inflammatory,
anti-virus, anti
microorganism
and anti-diabetes

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There are currently many
limitations in the chemical
synthesis of flavonoid C-
glycosides

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The enzyme mediated C-
glycosylation reaction exhibits high
efficiency and regional specificity

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- Trollius chinensis Bunge -
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- Lotus-TcCGT1 -
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Proved to be
the enzyme
that naturally
catalyzes
flavonoid 8C
glycosylation
reaction

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High affinity,
high catalytic
activity, and
broad-spectrum
catalytic activity
towards
flavonoids

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At the same time, research has
found a sucrose synthase that can
preferentially use UDP as a
substrate to produce UDPG,
providing glycosylation for
glycosylation reactions.

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if we couple
the two reactions,

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we will achieve higher
catalytic efficiency.

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But this sucrose synthase has a higher
optional catalytic temperature, so improving
the thermal stability of TcCGT1 is of great
help for the coupling of the reaction.

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