diff --git a/docs/project/results.md b/docs/project/results.md index 3e3fa03..8ae638c 100644 --- a/docs/project/results.md +++ b/docs/project/results.md @@ -60,7 +60,7 @@ To implement this system, we split the evolution process across three plasmids ( This modular system allows us to easily swap out the substrate on AP2, enabling it to be applied to different E3 ligases or substrates. It also supports performing “substrate walks,” a process where we incrementally alter the amino-acid sequence of the substrate recognition motif to shift from a canonical target to a novel target of therapeutic interest. By doing this stepwise, we can control the selection pressure and gradually evolve SIAH1/2 to recognize new substrates. -We plan to run this system in a bioreactor to create a continuous evolutionary environment. SIAH1/2 variants that successfully ubiquitinate the changing substrate will enable phage propagation, while less efficient variants are washed out (Figure X below). Over time, we can evolve SIAH1/2 to target a novel substrate, potentially demonstrating that directed evolution is a viable strategy for developing highly specific E3 ligases capable of precise targeted protein degradation. +We plan to run this system in a bioreactor to create a continuous evolutionary environment. SIAH1/2 variants that successfully ubiquitinate the changing substrate will enable phage propagation, while less efficient variants are washed out (Figure 4 below). Over time, we can evolve SIAH1/2 to target a novel substrate, potentially demonstrating that directed evolution is a viable strategy for developing highly specific E3 ligases capable of precise targeted protein degradation.
![Figure_positive_negative_selection](https://idec-teams.github.io/2024_Evolution_Suisse/img/PACE_related_schematics/Complete_E3_PACE.png)