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noemiesarah authored Oct 8, 2024
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Expand Up @@ -25,20 +25,20 @@ We are developing a novel approach that exploits the ability of the UPS to more
6. Further work towards clinical use of the evolved E3 ligase for Targeted Protein Degradation.

## Key achievements:
- We identified promising E3 ligases and targets.
- We have identified promising E3 ligases and targets.
- We developed an evolutionary logic to evolve these E3 ligases.
- We showed that phage propagation depends on the substrate used and the presence of both RNAP subunits.
- We observed a high background propagation in our system and we came up with possible solutions for the background phage propagation we observed.
- We observed a high background propagation in our system, and we developed possible solutions for the observed background phage propagation.
- We successfully drifted SIAH1.


We also:
- We learned the PACE workflow and practiced the setup and operation of the PACE reactor.
- We learned the PACE workflow and practiced setting up and running the PACE reactor.


We are currently still working on:
- Achieving E3 ligase activity-dependent phage propagation in the selection system.
- Running PANCE and/or PACE on
- Running PANCE and/or PACE to evolve SIAH1/2 towards the recognition of NLRP3.

## References
[^ubi_first]:Tsai JM, Nowak RP, Ebert BL, Fischer ES. Targeted protein degradation: from mechanisms to clinic. Nat Rev Mol Cell Biol. 2024;25: 740–757. doi:10.1038/s41580-024-00729-9
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