diff --git a/css/card.css b/css/card.css index 2701e69..524aaa1 100644 --- a/css/card.css +++ b/css/card.css @@ -176,8 +176,12 @@ .card-text-2 { position: absolute; +<<<<<<< HEAD left:280px; top: 20px; +======= + right:80px; +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e bottom: 50px; opacity: 0; } diff --git a/css/style.css b/css/style.css index 2919848..f2369f5 100644 --- a/css/style.css +++ b/css/style.css @@ -5,9 +5,14 @@ body{ position: fixed; font-family: 'Times New Roman', Times, serif; top: 14%; +<<<<<<< HEAD left: 10%; width: 80%; font-size: 80px; +======= + left: 2%; + font-size: 120px; +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e z-index: 9; color: #ffffff; transition: all 0.6s ease-in-out; diff --git a/index.html b/index.html index 97804f8..f178b6f 100644 --- a/index.html +++ b/index.html @@ -167,7 +167,11 @@
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Complete bio-degradation of PBAT
via evolved hydrolases and construction of an engineered Pseudomonas putida KT2440 strain

+======= +

Lorem ipsum dolor elit. Officia, voluptatibus!

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Development of a secretion pathway in Bacillus subtilis

We first established an efficient secretion system within Bacillus subtilis. The signal peptide AprE was fused to the N-terminus of the enzymes to enable them to be effectively secreted out of the cells via the Sec pathway. The successfully constructed protein exocytosis pathway allows for intuitive hydrolysis zones screening of the mutation libraries.

@@ -373,6 +401,22 @@

Development of an engineered Pseudomonas putida KT2440 strain

The evolved PBAT hydrolases were transformed into Pseudomonas putida KT2440-tph, which had undergone adaptive laboratory +======= +

Poly (butylene + adipate-co-terephthalate) (PBAT), a polymer synthesized from the monomers terephthalic acid (TPA), + adipic + acid (AA), and 1,4-butanediol (BDO), has garnered significant attention as a biocompatible and + degradable polymeric + material, exhibiting considerable potential for widespread application in the realm of agricultural film + technology.

+

We selected IsPETase, + BsLipA, and Lipase1028 as the starting points for directed evolution and established three + mutation libraries using error-prone PCR and site-directed mutagenesis. Hydrolysis zone screening method + was employed to + endow them with the ability to degrade PBAT.

+

The evolved PBAT hydrolases + were transformed into Pseudomonas putida KT2440-tph, which had undergone adaptive laboratory +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e evolution to acquire the ability to utilize terephthalic acid (TPA) and 1,4-butanediol (BDO) as carbon sources. This allowed the construction of a KT2440 strain with high PBAT degradation capacity and the ability to grow diff --git a/pages/member.html b/pages/member.html index 53bfba0..74641f2 100644 --- a/pages/member.html +++ b/pages/member.html @@ -151,6 +151,7 @@
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Xi Chen

LAB

+<<<<<<< HEAD

Performed the experiments, accounted for epPCR and screening.

+======= +

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+

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+

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
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Xi Chen

LAB

+<<<<<<< HEAD

Performed the whole experiments, analyzed the whole experimental data and made the key conclusion.

+======= +

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+

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+

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
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@@ -257,12 +305,25 @@

Xi Chen

LAB

+<<<<<<< HEAD

Guided the team, supported the experiment and wiki with rich experience with contributions from other authors.

+======= +

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e @@ -273,14 +334,27 @@

Xi Chen

LAB

+<<<<<<< HEAD

Supported the experiment with rich experience and wrote the article with contributions from other authors.

+======= +

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Xi Chen

LAB

+<<<<<<< HEAD

Performed the experiments, accounted for epPCR, site-mutagenesis and screening.

+======= +

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+

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+

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
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Xi Chen

LAB

+<<<<<<< HEAD

Designed the high-throughput screening method, performed the ALE of KT2440 strain experiments, and collected the background knowledge.

+======= +

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+

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Xi Chen

-24px;">LAB

+<<<<<<< HEAD

Supported the computational experiments with rich experience.

+======= +

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
@@ -334,12 +447,25 @@

Xi Chen

LAB

+<<<<<<< HEAD

Supported the experiment with rich experience and wrote the article with contributions from other authors.

+======= +

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+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
@@ -348,12 +474,25 @@

Xi Chen

LAB

+<<<<<<< HEAD

Performed the experiments, accounted for epPCR, site-mutagenesis and screening.

+======= +

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+

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e @@ -364,12 +503,25 @@

Xi Chen

LAB

+<<<<<<< HEAD

Guided the team, support the experiment with rich experience and wrote the article with contributions from other authors.

+======= +

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+

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e @@ -379,12 +531,25 @@

Xi Chen

MODEL

+<<<<<<< HEAD

Accounted for the computational experiments, including molecular dynamic simulations, modelling the structures of proteins and so on.

+======= +

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD

Designed the logo and poster for the team, made and beautified the wiki and slides.

+======= +

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
@@ -434,12 +612,25 @@

Xi Chen

DESIGN

+<<<<<<< HEAD

Designed the logo and poster for the team, made and beautified the wiki and slides, helped with the wiki and finished with the photo retouching work.

+======= +

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Xi Chen

DESIGN

+<<<<<<< HEAD

Designed the logo and poster for the team, made and beautified the wiki and slides.

+======= +

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+

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+

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e @@ -465,12 +669,25 @@

Xi Chen

WIKI

+<<<<<<< HEAD

Finished the wiki and provided useful wiki tools and suggestions.

+======= +

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+

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+

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
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Xi Chen

WIKI

+<<<<<<< HEAD

Finished the wiki and provided useful wiki tools and suggestions.

+======= +

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+

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+

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+>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e
+<<<<<<< HEAD +======= + +>>>>>>> 914ed4aa4dc10d7c1a2a0c39d938781f31bfb64e @@ -519,12 +749,21 @@

Xi Chen

+<<<<<<< HEAD
Image 1
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