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Solution

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Development of a secretion pathway in Bacillus subtilis

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Development of a secretion pathway in Bacillus subtilis

We first established an efficient secretion system within Bacillus subtilis. The signal peptide AprE was fused to the N-terminus of the enzymes to enable them to be effectively secreted out of the cells via the Sec pathway. The successfully constructed protein exocytosis pathway allows for intuitive hydrolysis zones screening of the mutation libraries.

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Development of PBAT hydrolases

We selected IsPETase, +

Development of PBAT hydrolases

We selected IsPETase, BsLipA, and Lipase1028 as the starting points for directed evolution and established three mutation libraries using error-prone PCR and site-directed mutagenesis. Hydrolysis zone screening method was employed to endow them with the ability to degrade PBAT.

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Development of an engineered Pseudomonas putida KT2440 strain

The evolved PBAT hydrolases +

Development of an engineered Pseudomonas putida KT2440 strain

The evolved PBAT hydrolases were transformed into Pseudomonas putida KT2440-tph, which had undergone adaptive laboratory evolution to acquire the ability to utilize terephthalic acid (TPA) and 1,4-butanediol (BDO) as carbon sources. This @@ -478,22 +483,22 @@

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Home

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Home

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Project

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Description

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Design

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Results

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Supplement

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Protocol

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Project

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Description

+

Design

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Results

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Supplement

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Protocol

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Lab Notebook

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Lab Notebook

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Team

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Collaboration

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Team

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Collaboration

diff --git a/js/movement.js b/js/movement.js index dbac7fe..473c712 100644 --- a/js/movement.js +++ b/js/movement.js @@ -160,6 +160,7 @@ window.addEventListener('scroll', function() { } else { // 如果滚动回到上方,物件恢复原位 MidBox.style.opacity=1; + } if (scrollPosition >250) { // 滚动到目标位置后,移动物件 @@ -167,6 +168,16 @@ window.addEventListener('scroll', function() { } else { // 如果滚动回到上方,物件恢复原位 TitBox.style.opacity=1; + + } + if (scrollPosition >260) { + // 滚动到目标位置后,移动物件 + TitBox.style.zIndex=-1; // 使用translate移动到新位置 + } else { + // 如果滚动回到上方,物件恢复原位 + TitBox.style.opacity=1; + TitBox.style.zIndex=6; + } diff --git "a/pages/NAU-CHINA-DE main 10.4 \347\273\210.pdf" "b/pages/NAU-CHINA-DE main 10.4 \347\273\210.pdf" deleted file mode 100644 index f2cf19a..0000000 Binary files "a/pages/NAU-CHINA-DE main 10.4 \347\273\210.pdf" and /dev/null differ diff --git a/pages/Pro.html b/pages/Pro.html index 628dd80..d1e35c6 100644 --- a/pages/Pro.html +++ b/pages/Pro.html @@ -55,7 +55,7 @@