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project-design.html
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<!DOCTYPE html>
<html>
<head>
<meta charset="UTF-8">
<link rel="stylesheet" href="bootstrap/css/bootstrap.min.css">
<link rel="stylesheet" type="text/css" href="css/swiper-bundle.min.css" />
<link rel="stylesheet" type="text/css" href="css/main.css" />
<link rel="stylesheet" type="text/css" href="css/team.css" />
<link rel="stylesheet" type="text/css" href="css/muban1.css" />
<script src="bootstrap/js/jquery.min.js"></script>
<script src="bootstrap/js/bootstrap.min.js"></script>
<script type="text/javascript" src="js/swiper-bundle.min.js"></script>
<script type="text/javascript" src="js/main.js"></script>
<script type="text/javascript" src="js/muban1.js"></script>
<title>ED-NAU Project Design</title>
<link rel="icon" href="./images/2021IDEClogo设计.png" type="image/x-icon">
</head>
<body>
<div class="nav col-lg-12 col-md-12 col-sm-12 col-xs-12">
<div class="logo_container col-lg-3 col-md-3 col-sm-3 col-xs-3">
<a href="./">
<img class="homepage_logo" src="./images/2021IDEClogo设计.png" alt="">
</a>
</div>
<div class="nav_cells col-lg-9 col-md-9 hidden-sm hidden-xs">
<ul>
<li class='nav_one hover_underline'>
<a href="./">HOME</a>
</li>
<li class='nav_one hover_underline'>
<a href="project-description">PROJECT</a>
<ul class="nav_two">
<li>
<a href="project-description" class="hover_underline">Description</a>
</li>
<li>
<a href="project-design" class="hover_underline">Design</a>
</li>
<li>
<a href="project-protocol" class="hover_underline">Protocol</a>
</li>
</ul>
</li>
<li class='nav_one hover_underline'>
<a href="results">RESULTS</a>
</li>
<li class='nav_one hover_underline'>
<a href="team-members">TEAM </a>
<ul class="nav_two">
<li>
<a href="team-members" class="hover_underline">Members</a>
</li>
<li>
<a href="team-collaborations" class="hover_underline">Collaborations</a>
</li>
<li>
<a href="team-attributions" class="hover_underline">Attributions</a>
</li>
<li>
<a href="team-notebook" class="hover_underline">Notebook</a>
</li>
</ul>
</li>
<li class='nav_one hover_underline'>
<a href="lab-notebook">LAB NOTEBOOK</a>
</li>
</ul>
</div>
<div class="nav_hidden nav_cells hidden-lg hidden-md col-sm-9 col-xs-9">
<ul>
<li class="nav_one"><img src="images/nav_little.png" alt="" style='width:7vw'>
<ul class="nav_two">
<li class="hover_underline">
<a href="./">HOME</a>
</li>
<li class="hover_underline">
<a href="project-description">PROJECT</a>
</li>
<li class="hover_underline">
<a href="results">RESULTS</a>
</li>
<li class="hover_underline">
<a href="team-members">TEAM</a>
</li>
<li class="hover_underline">
<a href="lab-notebook">LAB NOTEBOOK</a>
</li>
</ul>
</li>
</ul>
</div>
</div>
<!-- body -->
<div id="teambody">
<div class="swiper-container">
<div class="swiper-wrapper">
<div class="swiper-slide" style="background-image: url(images/project.jpg);display: flex;justify-content: center;align-items: center;">
<img src="./images/design.png" alt="" style="flex:1;">
</div>
</div>
<!-- Add Arrows -->
<div class="swiper-scrollbar"></div>
</div>
<div class="left_nav_container col-lg-3 col-md-3 hidden-sm hidden-xs">
<div class="left_nav">
<div class="left_nav_img"></div>
<a href="#Overview">Overview</a>
<a href="#Strain">Strain</a>
<a href="#Cd">Cd<sup>2+</sup></a>
<a href="#Mn">Mn<sup>2+</sup></a>
<a href="#Application">Application</a>
</div>
</div>
<div class="col-lg-9 col-md-9 col-sm-12 col-xs-12" style="background-color: white;padding: 0">
<div class="col-lg-1 col-md-1 hidden-sm hidden-xs"></div>
<div class="col-lg-8 col-md-8 col-sm-12 col-xs-12 content">
<a id='Overview' class="target-fix"></a>
<h2>Overview</h2>
<p>We integrated error-prone PCR, the phenotype of different concentration yeast under cadmium stress into our directed evolution strategy for transporter protein,OsNramp5,that transports Mn<sup>2+</sup> but not Cd<sup>2+</sup>.</p>
<div class="content_img"><img class="result_viewer" src="images/design1.png" alt="" style="width:50%"></div>
<div class="content_img"><img class="result_viewer" src="images/design2.png" alt=""></div>
<a id='Strain' class="target-fix"></a>
<h2>Strain</h2>
<p>In order to carry out the experiment smoothly, we conducted our study using the cadmium-sensitive strain (Δ<i>ycf1</i>) and the Mn<sup>2+</sup> channel-defected strain(Δ<i>smf1</i>)that are not able to carry Mn<sup>2+</sup>normally through
gene knockout. Δ<i>ycf1</i> is difficult to grow normally under cadmium stress, and due to the lack of the ability to transport Mn<sup>2+</sup>in Δsmf1, their growth capacity is usually worse than wild type. </p>
<div class="content_img"style="display: inline;float: left;">
<img class="result_viewer" src="images/ycf1.png" alt="" style="width:50%;display: inline;float: left;">
<img src="images/smf1.png" class="result_viewer"alt="" style="width:30%;display: inline;float: left;">
</div>
<a id="Cd" class="target-fix"></a>
<h2>Screening of OsNramp5-mut strains transferring less Cd<sup>2+</sup></h2>
<p>Recombinant plasmids were transformed into yeasts, and they could not grow on the solid SD-Ura media without this kind of plasmid. In the process of library construction, we got 260 positive transformants totally. Then, the single colony
of each transformant was propagated in liquid SD-Ura media. Different densities of yeast were obtained by gradient dilution, to spot on SD-Ura solid media containing <sup>2+</sup>. By doing so, the effect of different yeast concentration
was further avoided on the final phenotypic observation.</p>
<div class="content_img"><img class="result_viewer" src="images/screen1.png" alt=""></div>
<a id="Mn" class="target-fix"></a>
<h2>Screening of OsNramp5-mut strains transporting Mn<sup>2+</sup> normally</h2>
<p>The mutants with reduced transport of Cd<sup>2+</sup> selected in primary screening were treated as new experimental materials. They were spotted on Mn<sup>2+</sup>-deficient SD-Ura media with the control group (Δ<i>smf1-pFL61</i> and
Δ
<i>smf1-HR</i>). The Mn<sup>2+</sup>in media was specifically chelated by EGTA. As for the mutant strains, Δ<i>smf1-pFL61</i> could not grow well in the media while Δ<i>smf1-HR </i>can, because the Δ<i>smf1</i> strain cannot transport
Mn
<sup>2+</sup>normally, they already had an additional Mn<sup>2+</sup>OsNramp5 transporter whose ability of transporting Cd<sup>2+</sup> is decreased.</p>
<div class="content_img"><img class="result_viewer" src="images/screen2.png" alt=""></div>
<a id="Application" class="target-fix"></a>
<h2>Application</h2>
<p>The problem that Cd<sup>2+</sup>enriched in rice do harm to humans is still unresolved, and our subject holds the promise of achieving the evolution of OsNramp5 into an ideal Mn<sup>2+</sup>-specific transporter protein and therefore solving
the Cd<sup>2+</sup> rice problem at the source. We hope that this study will accelerate the genetic breeding of low or even cadmium-free rice and promote the utilization of cadmium-contaminated land and agricultural economic development.</p>
<div class="content_img"><img class="result_viewer" src="images/好笑的图.jpg" alt="" style="width:50%"></div>
</div>
<div id="ShowImage_Form" class="modal">
<div class="modal-body">
<div id="img_show">
</div>
</div>
</div>
</div>
</div>
<div class="footer">
<div class="footer_img col-lg-3 hidden-md hidden-sm hidden-xs"></div>
<div class="footer_menu col-lg-8 col-md-12 col-sm-12 col-xs-12">
<div class="footer_list ">
<div class="footer_title ">
<a href="./ ">
<div class="footer_arrow ">—</div>
<div> HOME</div>
</a>
</div>
</div>
<div class="footer_list ">
<div class="footer_title ">
<a href="project-description ">
<div class="footer_arrow ">—</div>
<div> PROJECT</div>
</a>
</div>
<div class="footer_title ">
<a href="project-description ">
<div class="footer_arrow ">—</div>
<div> Description</div>
</a>
</div>
<div class="footer_title ">
<a href="project-design ">
<div class="footer_arrow ">—</div>
<div> Design</div>
</a>
</div>
<div class="footer_title ">
<a href="project-PROTOCOL ">
<div class="footer_arrow ">—</div>
<div> Protocol</div>
</a>
</div>
</div>
<div class="footer_list ">
<div class="footer_title ">
<a href="results ">
<div class="footer_arrow ">—</div>
<div> RESULTS</div>
</a>
</div>
</div>
<div class="footer_list ">
<div class="footer_title ">
<a href="team-members ">
<div class="footer_arrow ">—</div>
<div> TEAM</div>
</a>
</div>
<div class="footer_title ">
<a href="team-members ">
<div class="footer_arrow ">—</div>
<div> Members</div>
</a>
</div>
<div class="footer_title ">
<a href="team-collaborations ">
<div class="footer_arrow ">—</div>
<div> Collaborations</div>
</a>
</div>
<div class="footer_title ">
<a href="team-attributions ">
<div class="footer_arrow ">—</div>
<div> Attributions</div>
</a>
</div>
<div class="footer_title ">
<a href="team-notebook ">
<div class="footer_arrow ">—</div>
<div> Notebook</div>
</a>
</div>
</div>
<div class="footer_list ">
<div class="footer_title ">
<a href="lab-notebook">
<div class="footer_arrow ">—</div>
<div> LAB NOTEBOOK</div>
</a>
</div>
</div>
</div>
</div>
<script type="text/javascript" src="js/team.js"></script>
</body>
</html>