diff --git a/src/dvclive/live.py b/src/dvclive/live.py
index c0b4aa8..f9726ed 100644
--- a/src/dvclive/live.py
+++ b/src/dvclive/live.py
@@ -82,7 +82,7 @@ def __init__(
         resume: bool = False,
         report: Literal["md", "notebook", "html", None] = None,
         save_dvc_exp: bool = True,
-        dvcyaml: Optional[str] = "dvc.yaml",
+        dvcyaml: Optional[str, Path] = "dvc.yaml",
         cache_images: bool = False,
         exp_name: Optional[str] = None,
         exp_message: Optional[str] = None,
@@ -104,11 +104,11 @@ def __init__(
                 part of `Live.end()`. Defaults to `True`. If you are using DVCLive
                 inside a DVC Pipeline and running with `dvc exp run`, the option will be
                 ignored.
-            dvcyaml (str | None): where to write dvc.yaml file, which adds DVC
+            dvcyaml (str | Path | None): where to write dvc.yaml file, which adds DVC
                 configuration for metrics, plots, and parameters as part of
                 `Live.next_step()` and `Live.end()`. If `None`, no dvc.yaml file is
                 written. Defaults to `"dvc.yaml"`. See `Live.make_dvcyaml()`.
-                If a string like `"subdir/dvc.yaml"`, DVCLive will write the
+                If a string or Path like `"subdir/dvc.yaml"`, DVCLive will write the
                 configuration to that path (file must be named "dvc.yaml").
                 If `False`, DVCLive will not write to "dvc.yaml" (useful if you are
                 tracking DVCLive metrics, plots, and parameters independently and
@@ -265,10 +265,14 @@ def _init_dvc(self):  # noqa: C901
             self._include_untracked.append(self.dir)
 
     def _init_dvc_file(self) -> str:
+        if isinstance(self._dvcyaml, Path):
+            self._dvcyaml = str(self._dvcyaml)
         if isinstance(self._dvcyaml, str):
             if os.path.basename(self._dvcyaml) == "dvc.yaml":
                 return self._dvcyaml
             raise InvalidDvcyamlError
+        if self._dvcyaml:
+            raise InvalidDvcyamlError("DVC yaml path is invalid. Must be a string or a Path object.")
         return "dvc.yaml"
 
     def _init_dvc_pipeline(self):
@@ -334,6 +338,8 @@ def _init_test(self):
         """
         with tempfile.TemporaryDirectory() as dirpath:
             self._dir = os.path.join(dirpath, self._dir)
+            if isinstance(self._dvcyaml, Path):
+                self._dvcyaml = str(self._dvcyaml)
             if isinstance(self._dvcyaml, str):
                 self._dvc_file = os.path.join(dirpath, self._dvcyaml)
             self._save_dvc_exp = False
diff --git a/tests/test_dvc.py b/tests/test_dvc.py
index bf263fc..e956323 100644
--- a/tests/test_dvc.py
+++ b/tests/test_dvc.py
@@ -1,6 +1,7 @@
 import os
 
 import pytest
+from pathlib import Path
 from dvc.exceptions import DvcException
 from dvc.repo import Repo
 from dvc.scm import NoSCM
@@ -220,12 +221,13 @@ def test_get_exp_name_duplicate(tmp_dir, mocked_dvc_repo, mocker, caplog):
     assert msg in caplog.text
 
 
-def test_test_mode(tmp_dir, monkeypatch, mocked_dvc_repo):
+@pytest.mark.parametrize("dvcyaml_path", ["dvc.yaml", Path("dvcyaml/dvc.yaml")])
+def test_test_mode(tmp_dir, monkeypatch, mocked_dvc_repo, dvcyaml_path):
     monkeypatch.setenv(DVCLIVE_TEST, "true")
-    live = Live("dir", dvcyaml="dvc.yaml")
+    live = Live("dir", dvcyaml=dvcyaml_path)
     live.make_dvcyaml()
     assert live._dir != "dir"
     assert live._dvc_file != "dvc.yaml"
     assert live._save_dvc_exp is False
     assert not os.path.exists("dir")
-    assert not os.path.exists("dvc.yaml")
+    assert not os.path.exists(dvcyaml_path)
\ No newline at end of file