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run_touch_hyperplane.m
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function run_touch_hyperplane(mouseName, sessionName, bSession, whiskerBaseDir, ppm, varargin)
% For 2pad (2-port angle distance), WT, WST, and WL is generated during prebuild_JK.m
%% Setup whisker array builder
useGPU = 0;
options.WindowStyle = 'normal';
optionsFin.WindowStyle = 'normal';
optionsFin.Units = 'normalized';
optionsFin.Position = [0.3 0.3 0.2 0.2];
try
whisker_d = [whiskerBaseDir mouseName sessionName '\'];
cd(whisker_d)
wstFnList = dir('*_WST.mat');
wstNums = zeros(length(wstFnList),1);
for wi = 1 : length(wstFnList)
wstNums(wi) = str2double(wstFnList(wi).name(1:end-8));
end
catch
error('No WST files')
end
if nargin == 6
skip = varargin{1};
else
skip = 'skip';
end
if strcmp(skip,'skip') && ~isempty(dir([whisker_d, '*touch_hp.mat']))
disp('Hyperplane already exists. Exit.')
return
end
savefn = [whisker_d mouseName sessionName '_touch_hp.mat'];
if strcmp(sessionName,'S99')
sessionName = 'S17';
elseif strcmp(sessionName,'S91')
sessionName = 'S01';
end
filelist=dir([whisker_d '*.measurements']);
dirTrialNums=zeros(1,size(filelist,1));
% trialNums=[]; % enter which trial nums to process
% %%
% Assign the trial numbers to existing .measurements files in the directory
% NOTE : This assumes that the .measurements files have leading numbers
% corresponding to trial number in string positions 1:end-13 of the file
% name. These index numbers may need to be changed to match up to the
% numerical code of the trial number. (2016/09/05 JK)
for i=1:length(filelist)
dirTrialNums(i)=str2double(filelist(i).name(1:end-13)); % extract out the trial number from each measurements file present in directory
end
trialNums = sort(dirTrialNums);
trialNums = trialNums(~isnan(trialNums));
trialNums = intersect(trialNums,bSession.trialNums); % try only the ones with behavior trials
includef=cell(size(trialNums,1),1);
for i = 1: length(trialNums)
includef{i} = num2str(trialNums(i));
end
% %% Make whisker-pole touch space for each type of trial, from 10 randomly selected trials (of each type)
% Currently, only dealing with 4 types of trials: 'rc', 'rf', 'lc', 'lf'
% Should make something different for straight pole touch in S00.
% 2017/04/11 JK
% Modifying to deal with different types of session, such as DISCRETE
% target angles and distractors.
% 2018/02/26 JK
% Determining the type of task and number of trial types within this session
servo_values = cellfun(@(x) x.servoAngle, bSession.trials);
servo_values = unique(servo_values(2:end)); % excluding trial #1, which is a dummy trial
distance_values = cellfun(@(x) x.motorDistance, bSession.trials);
distance_values = unique(distance_values(2:end)); % excluding trial #1, which is a dummy trial
distance_values((distance_values == 0)) = []; % excluding catch trials
servo_distance_pair = cell(length(servo_values),length(distance_values));
for i = 1 : length(servo_values)
for j = 1 : length(distance_values)
servo_distance_pair{i,j} = [servo_values(i), distance_values(j)];
end
end
% Initialize
steps_hp = cell(length(servo_values),length(distance_values));
num_points_in_hp = cell(length(servo_values),length(distance_values));
psi1 = zeros(length(servo_values),length(distance_values));
psi2 = zeros(length(servo_values),length(distance_values));
touch_hp = cell(length(servo_values),length(distance_values)); % touch hyperplanes
hp_peaks = cell(length(servo_values),length(distance_values)); % touch hyperplane peak points. 2 points for each hyperplane
trial_nums = cell(length(servo_values),length(distance_values));
thPolygon = cell(length(servo_values),length(distance_values));
% thflist = dir([whisker_d, '*touch_hp.mat']);
% if ~isempty(thflist)
% load(thflist(1).name);
% end
%%
for iservo = 1 : length(servo_values)
% for iservo = 5
for idist = 1 : length(distance_values)
%%
tt_ind = intersect(find(cellfun(@(x) (x.servoAngle == servo_values(iservo)),bSession.trials)), find(cellfun(@(x) (x.motorDistance == distance_values(idist)),bSession.trials)));
tt_wst_ind = find(cellfun(@(x) ismember(x.trialNum, wstNums), bSession.trials));
tt_ind = intersect(tt_ind, tt_wst_ind);
if isempty(tt_ind)
continue
end
temp_files = cell(length(tt_ind),1);
trial_nums{iservo,idist} = zeros(length(tt_ind),1);
poleTipCoords = zeros(length(tt_ind),2); % [x coordinates, y coordinates] of poleUpFrames (average them)
apPosition = zeros(length(tt_ind),1); % ap motor position from the behavior file
for j = 1 : length(tt_ind)
temp_files{j} = num2str(bSession.trials{tt_ind(j)}.trialNum);
trial_nums{iservo,idist}(j) = bSession.trials{tt_ind(j)}.trialNum;
load([temp_files{j},'_WST.mat'], 'ws') % loading ws
poleTipCoords(j,:) = mean(ws.topPix(ws.poleUpFrames,:));
apPosition(j) = bSession.trials{tt_ind(j)}.motorApPosition;
end
ws = Whisker.WhiskerSignalTrialArray_2pad(whisker_d,'include_files',temp_files);
done_flag = 1;
psi1_polygon_answer = 'Yes'; % for re-drawing of polygon for psi1
psi2Flip = 0;
if length(unique(apPosition)) == 1 % try in 2D plot
ind_opt = 1;
intersect_3d_total = [];
for tnum = 1 : length(ws.trials)
topInd = find(~isnan(ws.trials{tnum}.whiskerEdgeCoord(:,1)));
frontInd = find(~isnan(ws.trials{tnum}.whiskerEdgeCoord(:,2)));
noNaNInd = intersect(ws.trials{tnum}.poleUpFrames, intersect(topInd, frontInd));
intersect_3d_total = [intersect_3d_total; ws.trials{tnum}.whiskerEdgeCoord(noNaNInd,1), ws.trials{tnum}.whiskerEdgeCoord(noNaNInd,2), zeros(length(noNaNInd),1)];
end
psi1_answer = 'Yes';
while strcmp(psi1_answer, 'Yes')
disp('Calculating psi for a single ap position session')
angle_steps_pre = 1 : 185;
stds_pre = zeros(length(angle_steps_pre),1);
for i = 1 : length(angle_steps_pre)
A = viewmtx(angle_steps_pre(i),0);
x4d = [intersect_3d_total, ones(size(intersect_3d_total,1),1)]';
x2d = A*x4d;
x2d_pix = [floor(x2d(1,:));floor(x2d(2,:)/100)];
x2d_dim = [max(x2d_pix(2,:)) - min(x2d_pix(2,:)) + 1, max(x2d_pix(1,:)) - min(x2d_pix(1,:)) + 1];
x2d_proj = zeros(x2d_dim);
j_offset = min(x2d_pix(1,:)) - 1;
i_offset = min(x2d_pix(2,:)) - 1;
for j = 1 : size(x2d_pix,2)
x2d_proj(x2d_pix(2,j) - i_offset, x2d_pix(1,j) - j_offset) = x2d_proj(x2d_pix(2,j) - i_offset, x2d_pix(1,j) - j_offset) + 1;
end
stds_pre(i) = std(x2d_proj(x2d_proj(:)~=0));
end
[~, I] = findpeaks(smooth(smooth(stds_pre)),'sortstr','descend');
max_psi1_pre = angle_steps_pre(I(ind_opt));
h2 = figure('units','normalized','outerposition',[0.5 0 0.5 1]); subplot(1,2,1), plot(1:length(stds_pre), smooth(smooth(stds_pre,5))), hold on, plot(I(ind_opt),stds_pre(I(ind_opt)),'ro')
subplot(1,2,2), plot(1:length(stds_pre), stds_pre)
max_std = 0;
angle_steps = max_psi1_pre-0.9:0.1:max_psi1_pre+0.9; % resolution reduced to 0.1 from 0.01, flanking boundaries to 1 from 5 degrees for the sake of computation time 180414 JK
stds = zeros(length(angle_steps),1);
x2d_final = zeros(size(x2d_proj));
for i = 1:length(angle_steps)
A = viewmtx(angle_steps(i),0);
x4d = [intersect_3d_total, ones(size(intersect_3d_total,1),1)]';
x2d = A*x4d;
x2d_pix = [floor(x2d(1,:));floor(x2d(2,:)/100)]; % shrinking down to 100 times for better visualization
x2d_dim = [max(x2d_pix(2,:)) - min(x2d_pix(2,:)) + 1, max(x2d_pix(1,:)) - min(x2d_pix(1,:)) + 1];
x2d_proj = zeros(x2d_dim);
j_offset = min(x2d_pix(1,:)) - 1;
i_offset = min(x2d_pix(2,:)) - 1;
for j = 1 : length(x2d_pix)
x2d_proj(x2d_pix(2,j) - i_offset, x2d_pix(1,j) - j_offset) = x2d_proj(x2d_pix(2,j) - i_offset, x2d_pix(1,j) - j_offset) + 1;
end
temp_std = std(x2d_proj(x2d_proj(:)~=0));
stds(i) = temp_std;
if temp_std > max_std
max_std = temp_std;
psi1(iservo,idist) = angle_steps(i);
x2d_final = x2d_proj;
end
end
psi1(iservo,idist) = psi1(iservo,idist)-90;
subplot(1,2,2), plot(1:length(stds), stds)
A = viewmtx(psi1(iservo,idist)+90,90);
x4d = [intersect_3d_total, ones(size(intersect_3d_total,1),1)]';
x2d = A*x4d;
h3 = figure('WindowStyle','normal','units','normalized','outerposition',[0 0 1 1]); plot(x2d(1,:), x2d(2,:),'k.', 'MarkerSize',3)
answer = MFquestdlg([0.5, 0.3], 'Is psi correct?', 'whisker-pole intersection coordinate scatter', 'Yes', 'No', 'Yes');
switch answer
case 'Yes'
close all
psi1_answer = 'No'; % get out of this large while loop
case 'No'
close(h2), close(h3)
ind_opt = ind_opt + 1;
if length(I) >= ind_opt
continue
else
psi1_answer = MFquestdlg([0.5, 0.3],'Do you want to set (change) polygon drawing?', 'psi1 polygon', 'Yes', 'No', 'Yes');
if strcmp(psi1_answer,'Yes')
answer = 'Yes'; % to get out of innner while loop
psi1_polygon_answer = 'No';
else
error(['No observable psi1 at ' sessionName ' of ' mouseName])
end
end
end
end
psi2(iservo, idist) = 0;
%%
close all
%% Calculate touch hyperplanes
answer7 = 'Yes';
while strcmp(answer7, 'Yes')
answer7 = 'No'; % stay in this while loop only when certain condition is met (psi2 flip for some 90 degrees)
disp('Sweeping the hyperplane')
maxdist = ceil(sqrt(max(intersect_3d_total(:,1).^2) + max(intersect_3d_total(:,2).^2))); xmin = -maxdist; xmax = maxdist;
zmin_data = min(intersect_3d_total(:,3)); zmax_data = max(intersect_3d_total(:,3)); zdiff = zmax_data - zmin_data; zmax = zmax_data + zdiff; zmin = zmin_data - zdiff;
ymin_data = min(intersect_3d_total(:,2)); ymax_data = max(intersect_3d_total(:,2));
z = zmin:zmax;
xyz = zeros((length(z))*(xmax-xmin+1),3);
for i = xmin:xmax
xyz((i-xmin)*length(z)+1 : (i-xmin+1)*length(z),:) = [ones(length(z),1)*i + mean(intersect_3d_total(:,1)), zeros(length(z),1) + mean(intersect_3d_total(:,2)), z'];
end
hp_decision = 'No';
while(strcmp(hp_decision,'No'))
% [xyz_psi1, ~, ~] = AxelRot(xyz',psi1(iservo,idist),[0 0 1], 0); % rotate psi1 degrees counterclockwise around z axis
[xyz_psi1, ~, ~] = AxelRot(xyz',psi1(iservo,idist),[0 0 1], [mean(intersect_3d_total(:,1)) mean(intersect_3d_total(:,2)) 0]); % rotate psi1 degrees counterclockwise around z axis
zcenter = floor(mean([zmax_data, zmin_data]));
% x0 = [0 0 zcenter];
x0 = [mean(intersect_3d_total(:,1)) mean(intersect_3d_total(:,2)) zcenter];
if psi1(iservo,idist) == 90
u = [0 1 0];
else
u = [1 tand(psi1(iservo,idist)) 0];
end
[xyz_psi2, ~, ~] = AxelRot(xyz_psi1, psi2(iservo,idist), u, x0);
xyz_psi2(:,xyz_psi2(3,:) < zmin_data) = [];
xyz_psi2(:,xyz_psi2(3,:) > zmax_data) = [];
xyz_psi2(:,xyz_psi2(2,:) < ymin_data) = [];
xyz_psi2(:,xyz_psi2(2,:) > ymax_data) = [];
if isempty(xyz_psi2)
[xyz_psi2, ~, ~] = AxelRot(xyz_psi1, psi2(iservo,idist), u, x0);
xyz_psi2(:,xyz_psi2(2,:) < ymin_data) = [];
xyz_psi2(:,xyz_psi2(2,:) > ymax_data) = [];
end
%%
% figure, plot3(intersect_3d_total(:,1),intersect_3d_total(:,2), intersect_3d_total(:,3),'k.', 'MarkerSize',3), xlabel('top'), ylabel('front'), zlabel('pos'), hold on
% zmaxind = find(xyz_psi2(3,:) == zmax_data); zminind = find(xyz_psi2(3,:) == zmin_data);
% for zi = 1 : length(zmaxind)
% xind = find(abs(xyz_psi2(1,:) - xyz_psi2(1,zmaxind(zi))) < 1);
% [~, minzind] = min(xyz_psi2(3,xind));
% plot3(xyz_psi2(1,[zmaxind(zi), xind(minzind)]), xyz_psi2(2,[zmaxind(zi), xind(minzind)]), xyz_psi2(3,[zmaxind(zi), xind(minzind)]), 'r-')
% end
%% test
figure('units','normalized','outerposition',[0 0.3 0.5 0.5]),
plot3(intersect_3d_total(:,1),intersect_3d_total(:,2), intersect_3d_total(:,3),'k.', 'MarkerSize',3), xlabel('top'), ylabel('front'), zlabel('pos'), hold on
plot3(xyz_psi2(1,:), xyz_psi2(2,:), xyz_psi2(3,:), 'r.')
%% ~ 0.5 min (depending on the length of "steps" and the size of xyz_psi2)
intersect_pix = round(intersect_3d_total);
if psi2Flip
A = viewmtx(psi1(iservo,idist),-90+psi2(iservo,idist));
else
A = viewmtx(psi1(iservo,idist),90-psi2(iservo,idist));
end
intersect_4d = [intersect_3d_total, ones(size(intersect_pix,1),1)]';
intersect_2d = A*intersect_4d;
intersect_2d = unique(round(intersect_2d(1:2,:)',2),'rows');
if isempty(steps_hp{iservo, idist})
steps_hp{iservo, idist} = round(-ppm*4 : -ppm*2 + 20);
end
num_points = zeros(length(steps_hp{iservo, idist}),1);
% steps = steps_hp{iservo, idist};
% parfor i = 1:length(steps) % this is time consuming...
parfor i = 1:length(steps_hp{iservo, idist})
% for i = 1:length(steps_hp{iservo, idist})
hp = round(xyz_psi2);
% hp(1,:) = hp(1,:)+ steps(i);
% hp_4d = [hp; ones(1,size(hp,2))];
% hp_2d = A*hp_4d;
% hp_2d = hp_2d(1:2,:)';
% num_points(i) = sum(ismember(intersect_2d, hp_2d,'rows'));
hp(1,:) = hp(1,:)+ steps_hp{iservo, idist}(i);
num_points(i) = sum(ismember(intersect_pix, hp','rows'));
end
h1 = figure('WindowStyle','normal','units','normalized','outerposition',[0.5 0.3 0.5 0.5]); plot(steps_hp{iservo, idist},num_points(:), 'k-', 'LineWidth', 3), xlabel('translocation (pix)'), ylabel('# intersection')
answer = MFquestdlg([0.5, 0.3], 'Does the result look correct?', 'Touch hyperplane peaks', 'Yes', 'No', 'Yes');
peak_answer = 'Yes';
while(strcmp(peak_answer,'Yes'))
if strcmp(answer,'Yes')
while true
datacursormode(h1,'on')
hp_peak_ans = inputdlg({'Left peak','Right peak'},'What are the peak points?',1,{'',''},optionsFin);
if ~isempty(hp_peak_ans) && ~isempty(hp_peak_ans{1}) && ~isempty(hp_peak_ans{2})
break
end
end
hp_peaks{iservo, idist} = [str2double(hp_peak_ans{1}) str2double(hp_peak_ans{2})];
% Final confirmation
% project the peak hyperplanes and all coordinates onto psi1 psi2 view
%%
h2 = figure('units','normalized','outerposition',[0 0 1 1]);
th_4d1 = [xyz_psi2(1,:) + hp_peaks{iservo, idist}(1);xyz_psi2(2:3,:);ones(1,size(xyz_psi2,2))];
th_2d1 = A*th_4d1;
th_2d1 = unique(th_2d1(1:2,:)','rows');
th_4d2 = [xyz_psi2(1,:) + hp_peaks{iservo, idist}(2);xyz_psi2(2:3,:);ones(1,size(xyz_psi2,2))];
th_2d2 = A*th_4d2;
th_2d2 = unique(th_2d2(1:2,:)','rows');
scatter(intersect_2d(:,1),intersect_2d(:,2),'k.'), hold on, scatter(th_2d1(:,1), th_2d1(:,2),'r.'), scatter(th_2d2(:,1), th_2d2(:,2),'r.')
%%
answer2 = MFquestdlg([0.5, 0.3], 'Is the result REALLY correct?', 'Final Confirmation', 'Yes', 'No', 'Yes');
switch answer2
case 'Yes'
close(h2);
peak_answer = 'No'; % get out of peak while
hp_decision = 'Yes';
done_flag = 0; % the whole precedure is finally done. get out of while(done_flag) loop.
case 'No'
answer3 = MFquestdlg([0.5, 0.3], 'Do you want to change the steps?', 'Touch hyperplane sweep steps', 'Yes', 'No', 'Yes');
switch answer3
case 'Yes'
step_boundary_cell = inputdlg({'First step','Last step'},'What are the sweep boundaries?',1,{'',''},options);
steps_hp{iservo, idist} = str2double(step_boundary_cell{1}):str2double(step_boundary_cell{2});
peak_answer = 'No'; % get out of peak while
close all
case 'No'
peak_answer = MFquestdlg([0.5, 0.3], 'Do you want to change the peak points?', 'Touch hyperplane peaks', 'Yes', 'No', 'Yes');
if strcmp(peak_answer,'Yes')
close(h2);
else
psi1_return_answer = MFquestdlg([0.5, 0.3], 'Do you want to draw region for psi1?', 'Return to psi1 region selection', 'Yes', 'No', 'Yes');
if strcmp(psi1_return_answer, 'Yes')
close all
psi1_polygon_answer = 'No';
peak_answer = 'No';
hp_decision = 'Yes';
else
psi1_adjust = inputdlg('How much change in psi1? (Negative for CW)', 'psi1 manual adjustment', 1, {''}, options);
if psi1_adjust{1}
psi1(iservo,idist) = psi1(iservo,idist) + str2double(psi1_adjust{1});
else
error(['No optimal hyperplanes peaks found in ' sessionName ' of ' mouseName])
end
end
end
end
end
else % answer = 'No' to question 'Does the result look correct?'
answer3 = MFquestdlg([0.5, 0.3], 'Do you want to change the steps?', 'Touch hyperplane sweep steps', 'Yes', 'No', 'Yes');
switch answer3
case 'Yes'
step_boundary_cell = inputdlg({'First step','Last step'},'What are the sweep boundaries?',1,{'',''},options);
steps_hp{iservo, idist} = str2double(step_boundary_cell{1}):str2double(step_boundary_cell{2});
peak_answer = 'No';
answer7 = 'No';
close all
case 'No'
answer7 = MFquestdlg([0.5, 0.3], 'Do you want to flip psi2?', 'Wierd psi2 error', 'Yes', 'No', 'Yes');
switch answer7
case 'Yes'
psi2Flip = 1-psi2Flip;
psi2(iservo, idist) = -psi2(iservo, idist);
peak_answer = 'No';
case 'No'
% questTitle = 'Return to psi1 polygon';
% start(timer('StartDelay',1,'TimerFcn',@(o,e)set(findall(0,'Tag',questTitle),'WindowStyle','normal')));
psi1_return_answer = MFquestdlg([0.5, 0.3], 'Do you want to draw a region for psi1?', 'Return to psi1 region selection', 'Yes', 'No', 'Yes');
if strcmp(psi1_return_answer, 'Yes')
close all
psi1_polygon_answer = 'No';
peak_answer = 'No';
hp_decision = 'Yes';
else
psi1_adjust = inputdlg('How much change in psi1?', 'psi1 manual adjustment', 1, {''}, options);
if psi1_adjust{1}
psi1(iservo,idist) = psi1(iservo,idist) + str2double(psi1_adjust{1});
else
error(['No optimal hyperplanes peaks found in ' sessionName ' of ' mouseName])
end
end
end
end
end
end
end
end
else
while (done_flag)
intersect_3d_total = [];
for tnum = 1 : length(ws.trials)
topInd = find(~isnan(ws.trials{tnum}.whiskerEdgeCoord(:,1)));
frontInd = find(~isnan(ws.trials{tnum}.whiskerEdgeCoord(:,2)));
noNaNInd = intersect(ws.trials{tnum}.poleUpFrames, intersect(topInd, frontInd));
intersect_3d_total = [intersect_3d_total; ws.trials{tnum}.whiskerEdgeCoord(noNaNInd,1), ws.trials{tnum}.whiskerEdgeCoord(noNaNInd,2), ws.trials{tnum}.apPosition(noNaNInd)];
end
intersect_3d_total = unique(round(intersect_3d_total,2),'rows');
psi1_answer = 'Yes'; % for overall psi1 detection
while(strcmp(psi1_answer,'Yes'))
while(strcmp(psi1_polygon_answer,'No'))
h2 = figure('units','normalized','outerposition',[0 0 1 1]); plot(intersect_3d_total(:,1), intersect_3d_total(:,2), 'k.', 'MarkerSize', 0.1), hold on
pre_poly = []; % points of the polygon
i = 1;
temp_point = ginput(1);
while(~isempty(temp_point)) % finish drawing polygon by pressing "enter"
pre_poly = [pre_poly; temp_point];
plot(pre_poly(i,1), pre_poly(i,2), 'bo', 'MarkerSize', 3)
if i > 1
plot(pre_poly(i-1:i,1), pre_poly(i-1:i,2), 'b-')
end
i = i + 1;
temp_point = ginput(1);
end
plot([pre_poly(end,1);pre_poly(1,1)], [pre_poly(end,2);pre_poly(1,2)], 'b-')
% questTitle='Polygon pre-selection';
% start(timer('StartDelay',1,'TimerFcn',@(o,e)set(findall(0,'Tag',questTitle),'WindowStyle','normal')));
psi1_polygon_answer = MFquestdlg([0.5,0.3], 'Is the drawing correct?', 'Region pre-selection', 'Yes', 'No', 'Yes');
switch psi1_polygon_answer
case 'Yes'
close all
in = inpolygon(intersect_3d_total(:,1), intersect_3d_total(:,2), pre_poly(:,1), pre_poly(:,2));
intersect_3d_crop = intersect_3d_total(in,:);
case 'No'
close(h2)
h2 = figure('units','normalized','outerposition',[0 0 1 1]); plot(intersect_3d_total(:,1), intersect_3d_total(:,2), 'k.', 'MarkerSize', 0.1), hold on
end
end
if exist('intersect_3d_crop','var')
intersect_3d = intersect_3d_crop;
clear intersect_3d_crop
else
intersect_3d = intersect_3d_total;
end
h1 = figure('units','normalized','outerposition',[0 0 1 1]); plot3(intersect_3d(:,1), intersect_3d(:,2), intersect_3d(:,3), 'k.', 'MarkerSize', 0.1)
title(['Angle = ', num2str(servo_values(iservo)), ', Distance = ', num2str(distance_values(idist))]), xlabel('Top-view intersection coord'), ylabel('Front-view intersection coord'), zlabel('Pole position')
%% when interested in certain points in the figure
% % 'oo': 245 246 353 382 436 558 559
%
% zvalue = 68000;
% tnumHigher = intersect(tt_ind, find(cellfun(@(x) x.motorApPosition < zvalue, bSession.trials)))
% tnumLower = intersect(tt_ind, find(cellfun(@(x) x.motorApPosition > zvalue, bSession.trials)))
% zvalue = 42410;
% tnum = intersect(tt_ind, find(cellfun(@(x) abs(x.motorApPosition - zvalue) < 10, bSession.trials)))
% tnum_ws = find(cellfun(@(x) x.trialNum == tnum(1), ws.trials))
% ws.trials{tnum_ws}.trackerFileName
% figure, plot3(ws.trials{tnum_ws}.whiskerEdgeCoord(:,1), ws.trials{tnum_ws}.whiskerEdgeCoord(:,2), 1:length(ws.trials{tnum_ws}.whiskerEdgeCoord(:,1)))
% xlabel('Top-view intersection coord'), ylabel('Front-view intersection coord'), zlabel('Frame #'), title(num2str(tnum))
%% Calculate psi1 % takes ~ 15 sec
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% Manual selection
ind_opt = 1; % optimal peak index. Starting from 1
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
disp('Calculating psi1')
% angle_steps_pre = -5 : 179;
angle_steps_pre = 1 : 185;
% angle_steps_pre = servo_values(iservo)+80 : servo_values(iservo)+105; % 180414 JK. contraint for initial guess to reduce computation time. From previous results of JK025 and JK027 with -5:185.
% angle_steps_pre = servo_values(iservo)-100 : servo_values(iservo)-75; % 180414 JK. contraint for initial guess to reduce computation time. From previous results of JK025 and JK027 with -5:185.
% Weird things happening with restricting pre angle steps. When angle_steps_pre > 180.
stds_pre = zeros(length(angle_steps_pre),1);
for i = 1 : length(angle_steps_pre)
A = viewmtx(angle_steps_pre(i),0);
x4d = [intersect_3d, ones(size(intersect_3d,1),1)]';
x2d = A*x4d;
x2d_pix = [floor(x2d(1,:));floor(x2d(2,:)/100)];
x2d_dim = [max(x2d_pix(2,:)) - min(x2d_pix(2,:)) + 1, max(x2d_pix(1,:)) - min(x2d_pix(1,:)) + 1];
x2d_proj = zeros(x2d_dim);
j_offset = min(x2d_pix(1,:)) - 1;
i_offset = min(x2d_pix(2,:)) - 1;
for j = 1 : size(x2d_pix,2)
x2d_proj(x2d_pix(2,j) - i_offset, x2d_pix(1,j) - j_offset) = x2d_proj(x2d_pix(2,j) - i_offset, x2d_pix(1,j) - j_offset) + 1;
end
stds_pre(i) = std(x2d_proj(x2d_proj(:)~=0));
end
[~, I] = findpeaks(smooth(smooth(stds_pre)),'sortstr','descend');
answer = 'No'; % first answer for psi1 (Is psi1 correct?)
while(strcmp(answer,'No'))
max_psi1_pre = angle_steps_pre(I(ind_opt));
h2 = figure('units','normalized','outerposition',[0.5 0 0.5 1]); subplot(1,2,1), plot(1:length(stds_pre), smooth(smooth(stds_pre,5))), hold on, plot(I(ind_opt),stds_pre(I(ind_opt)),'ro')
subplot(1,2,2), plot(1:length(stds_pre), stds_pre)
max_std = 0;
angle_steps = max_psi1_pre-0.9:0.1:max_psi1_pre+0.9; % resolution reduced to 0.1 from 0.01, flanking boundaries to 1 from 5 degrees for the sake of computation time 180414 JK
stds = zeros(length(angle_steps),1);
x2d_final = zeros(size(x2d_proj));
for i = 1:length(angle_steps)
A = viewmtx(angle_steps(i),0);
x4d = [intersect_3d, ones(size(intersect_3d,1),1)]';
x2d = A*x4d;
x2d_pix = [floor(x2d(1,:));floor(x2d(2,:)/100)]; % shrinking down to 100 times for better visualization
x2d_dim = [max(x2d_pix(2,:)) - min(x2d_pix(2,:)) + 1, max(x2d_pix(1,:)) - min(x2d_pix(1,:)) + 1];
x2d_proj = zeros(x2d_dim);
j_offset = min(x2d_pix(1,:)) - 1;
i_offset = min(x2d_pix(2,:)) - 1;
for j = 1 : length(x2d_pix)
x2d_proj(x2d_pix(2,j) - i_offset, x2d_pix(1,j) - j_offset) = x2d_proj(x2d_pix(2,j) - i_offset, x2d_pix(1,j) - j_offset) + 1;
end
temp_std = std(x2d_proj(x2d_proj(:)~=0));
stds(i) = temp_std;
if temp_std > max_std
max_std = temp_std;
psi1(iservo,idist) = angle_steps(i);
x2d_final = x2d_proj;
end
end
psi1(iservo,idist) = psi1(iservo,idist)-90;
subplot(1,2,2), plot(1:length(stds), stds)
A = viewmtx(psi1(iservo,idist)+90,0);
x4d = [intersect_3d, ones(size(intersect_3d,1),1)]';
x2d = A*x4d;
h3 = figure('WindowStyle','normal','units','normalized','outerposition',[0 0 1 1]); plot(x2d(1,:), x2d(2,:),'k.', 'MarkerSize',3)
answer = MFquestdlg([0.5, 0.3], 'Is psi1 correct?', 'whisker-pole intersection coordinate scatter', 'Yes', 'No', 'Yes');
switch answer
case 'Yes'
close all
psi1_answer = 'No'; % get out of this large while loop
case 'No'
close(h2), close(h3)
ind_opt = ind_opt + 1;
if length(I) >= ind_opt
continue
else
psi1_answer = MFquestdlg([0.5, 0.3],'Do you want to set (change) polygon drawing?', 'psi1 polygon', 'Yes', 'No', 'Yes');
if strcmp(psi1_answer,'Yes')
answer = 'Yes'; % to get out of innner while loop
psi1_polygon_answer = 'No';
else
error(['No observable psi1 at ' sessionName ' of ' mouseName])
end
end
end
end
end
%% Manual check of the view
% A = viewmtx(I(6)+5,0);
% x4d = [intersect_3d, ones(size(intersect_3d,1),1)]';
% x2d = A*x4d;
% h3 = figure; plot(x2d(1,:), x2d(2,:),'k.', 'MarkerSize',3)
%% Psi2
%%%%%%%%%%%%%%%%%%%%%%%%%%%% Manual selection
answer1 = 'No'; answer2 = 'Yes';
disp('Calculating psi2')
x2d_flip = flip(x2d_final,1);
while(strcmp(answer1,'No')) % answer1 = Is psi2 correct?
h2 = figure('units','normalized','outerposition',[0 0 1 1]); imagesc(x2d_flip), hold on
BW = zeros(size(x2d_flip)) + 1;
while(strcmp(answer2,'No')) % Draw polygon to select regions for radon transform
psi2_poly = [];
i = 1;
temp_point = ginput(1);
while(~isempty(temp_point)) % finish drawing polygon by pressing "enter"
psi2_poly = [psi2_poly;temp_point];
plot(psi2_poly(i,1), psi2_poly(i,2), 'yo', 'MarkerSize', 3)
if i > 1
plot(psi2_poly(i-1:i,1), psi2_poly(i-1:i,2), 'y-')
end
i = i + 1;
temp_point = ginput(1);
end
plot([psi2_poly(end,1);psi2_poly(1,1)], [psi2_poly(end,2);psi2_poly(1,2)], 'y-')
answer2 = MFquestdlg([0.5, 0.3], 'Is the polygon right?', 'Polygon pre-selection', 'Yes', 'No', 'Yes');
switch answer2
case 'Yes'
close all
[sub_1, sub_2] = ind2sub(size(x2d_flip),1:length(x2d_flip(:)));
in = inpolygon(sub_2, sub_1, round(psi2_poly(:,1)), round(psi2_poly(:,2)));
BW = zeros(size(x2d_flip));
BW(in) = 1;
case 'No'
close(h2)
h2 = figure('units','normalized','outerposition',[0 0 1 1]); imagesc(x2d_flip), hold on
end
end
x2d_edge = x2d_flip .* BW;
%%
theta = 0:0.01:180;
R = radon(x2d_edge, theta);
[~, max_ind] = max(std(R));
psi2(iservo,idist) = (max_ind-1)*0.01;
psi2(iservo,idist) = atand(tand(psi2(iservo,idist))/100); % psi2 adjusted because it was calculated with pole position divided by 100
linex = zeros(3,2);
x2d_flip_1strow = x2d_flip(1,:);
[~, linex(1,1)] = max(x2d_flip_1strow); x2d_flip_1strow(max(1,linex(1,1)-5):min(size(x2d_flip,2),linex(1,1)+5)) = 0;
[~, linex(2,1)] = max(x2d_flip_1strow); x2d_flip_1strow(max(1,linex(2,1)-5):min(size(x2d_flip,2),linex(2,1)+5)) = 0;
[~, linex(3,1)] = max(x2d_flip_1strow);
for i = 1 : 3
linex(i,2) = linex(i,1) + tand((max_ind-1)*0.01)*size(x2d_flip,1);
end
liney = [1 size(x2d_flip,1);1 size(x2d_flip,1);1 size(x2d_flip,1)];
figure('units','normalized','outerposition',[0 0 1 1]),
subplot(121), imagesc(R), xlabel('Angle = 0:0.01:180'), ylabel('Projected values'), axis square
subplot(122); imagesc(x2d_flip, [0 1000]), axis square, hold on,
for i = 1 : 3
line(linex(i,:),liney(i,:), 'LineWidth', 3, 'Color', [1 1 1])
end
answer1 = MFquestdlg([0.5, 0.3], 'Is psi2 correct?', 'whisker-pole intersection coordinate side-view', 'Yes', 'No', 'Yes');
switch answer1
case 'Yes'
close all
case 'No'
answer3 = MFquestdlg([0.5, 0.3], 'Do you want to draw a region again?', 'Re-drawing the region', 'Yes', 'No', 'Yes');
close all
switch answer3
case 'Yes'
answer2 = 'No';
case 'No'
answer4 = MFquestdlg([0.5, 0.3], 'Do you want to manually draw psi2?', 'Manual psi2', 'Yes', 'No', 'Yes');
if strcmp(answer4, 'Yes')
while (true)
figure('units','normalized','outerposition',[0 0 1 1])
imagesc(x2d_flip, [0 1000]), axis square, hold on,
[x,y] = ginput(2);
plot(x, y, 'wo-', 'MarkerSize', 5, 'LineWidth', 5)
answer5 = MFquestdlg([0.5, 0.3], 'Is this correct?', 'Manual psi2', 'Yes', 'No', 'Yes');
if strcmp(answer5, 'Yes')
angle = atand(diff(x)/diff(y)); % the image is 90 degrees rotated.
psi2(iservo, idist) = atand(tand(angle)/100);
answer1 = 'Yes';
break
else
answer6 = MFquestdlg([0.5, 0.3], 'Do you want to specify the angle?', 'psi2', 'Yes', 'No', 'Yes');
if strcmp(answer6, 'Yes')
psi2(iservo, idist) = str2double(inputdlg('psi2 angle', 'psi2', 1, {''}, options));
answer1 = 'Yes';
break
else
error(['No optimal psi2 found in ' sessionName ' of ' mouseName])
end
end
end
else
answer6 = MFquestdlg([0.5, 0.3], 'Do you want to specify the angle?', 'psi2', 'Yes', 'No', 'Yes');
if strcmp(answer6, 'Yes')
psi2(iservo,idist) = str2double(inputdlg('psi2 angle', 'psi2', 1, {''}, options));
answer1 = 'Yes';
break
else
error(['No optimal psi2 found in ' sessionName ' of ' mouseName])
end
end
end
end
if psi1(iservo,idist) > 90
psi2(iservo, idist) = -psi2(iservo, idist);
psi2Flip = 1;
end
end
close all
%% Calculate touch hyperplanes
answer7 = 'Yes';
while strcmp(answer7, 'Yes')
answer7 = 'No'; % stay in this while loop only when certain condition is met (psi2 flip for some 90 degrees)
disp('Sweeping the hyperplane')
maxdist = ceil(sqrt(max(intersect_3d_total(:,1).^2) + max(intersect_3d_total(:,2).^2))); xmin = -maxdist; xmax = maxdist;
zmin_data = min(intersect_3d_total(:,3)); zmax_data = max(intersect_3d_total(:,3)); zdiff = zmax_data - zmin_data; zmax = zmax_data + zdiff; zmin = zmin_data - zdiff;
ymin_data = min(intersect_3d_total(:,2)); ymax_data = max(intersect_3d_total(:,2));
z = zmin:zmax;
xyz = zeros((length(z))*(xmax-xmin+1),3);
for i = xmin:xmax
xyz((i-xmin)*length(z)+1 : (i-xmin+1)*length(z),:) = [ones(length(z),1)*i + mean(intersect_3d_total(:,1)), zeros(length(z),1) + mean(intersect_3d_total(:,2)), z'];
end
hp_decision = 'No';
while(strcmp(hp_decision,'No'))
% [xyz_psi1, ~, ~] = AxelRot(xyz',psi1(iservo,idist),[0 0 1], 0); % rotate psi1 degrees counterclockwise around z axis
[xyz_psi1, ~, ~] = AxelRot(xyz',psi1(iservo,idist),[0 0 1], [mean(intersect_3d_total(:,1)) mean(intersect_3d_total(:,2)) 0]); % rotate psi1 degrees counterclockwise around z axis
zcenter = floor(mean([zmax_data, zmin_data]));
% x0 = [0 0 zcenter];
x0 = [mean(intersect_3d_total(:,1)) mean(intersect_3d_total(:,2)) zcenter];
if psi1(iservo,idist) == 90
u = [0 1 0];
else
u = [1 tand(psi1(iservo,idist)) 0];
end
[xyz_psi2, ~, ~] = AxelRot(xyz_psi1, psi2(iservo,idist), u, x0);
xyz_psi2(:,xyz_psi2(3,:) < zmin_data) = [];
xyz_psi2(:,xyz_psi2(3,:) > zmax_data) = [];
xyz_psi2(:,xyz_psi2(2,:) < ymin_data) = [];
xyz_psi2(:,xyz_psi2(2,:) > ymax_data) = [];
if isempty(xyz_psi2)
[xyz_psi2, ~, ~] = AxelRot(xyz_psi1, psi2(iservo,idist), u, x0);
xyz_psi2(:,xyz_psi2(2,:) < ymin_data) = [];
xyz_psi2(:,xyz_psi2(2,:) > ymax_data) = [];
end
%%
% figure, plot3(intersect_3d_total(:,1),intersect_3d_total(:,2), intersect_3d_total(:,3),'k.', 'MarkerSize',3), xlabel('top'), ylabel('front'), zlabel('pos'), hold on
% zmaxind = find(xyz_psi2(3,:) == zmax_data); zminind = find(xyz_psi2(3,:) == zmin_data);
% for zi = 1 : length(zmaxind)
% xind = find(abs(xyz_psi2(1,:) - xyz_psi2(1,zmaxind(zi))) < 1);
% [~, minzind] = min(xyz_psi2(3,xind));
% plot3(xyz_psi2(1,[zmaxind(zi), xind(minzind)]), xyz_psi2(2,[zmaxind(zi), xind(minzind)]), xyz_psi2(3,[zmaxind(zi), xind(minzind)]), 'r-')
% end
%% test
figure('units','normalized','outerposition',[0 0.3 0.5 0.5]),
plot3(intersect_3d_total(:,1),intersect_3d_total(:,2), intersect_3d_total(:,3),'k.', 'MarkerSize',3), xlabel('top'), ylabel('front'), zlabel('pos'), hold on
plot3(xyz_psi2(1,:), xyz_psi2(2,:), xyz_psi2(3,:), 'r.')
%% ~ 0.5 min (depending on the length of "steps" and the size of xyz_psi2)
intersect_pix = round(intersect_3d_total);
if psi2Flip
A = viewmtx(psi1(iservo,idist),-90+psi2(iservo,idist));
else
A = viewmtx(psi1(iservo,idist),90-psi2(iservo,idist));
end
intersect_4d = [intersect_3d_total, ones(size(intersect_pix,1),1)]';
intersect_2d = A*intersect_4d;
intersect_2d = unique(round(intersect_2d(1:2,:)',2),'rows');
if isempty(steps_hp{iservo, idist})
steps_hp{iservo, idist} = -ppm*4 : -ppm*2 + 20;
steps_hp{iservo, idist} = round(steps_hp{iservo, idist});
end
num_points = zeros(length(steps_hp{iservo, idist}),1);
% steps = steps_hp{iservo, idist};
% parfor i = 1:length(steps) % this is time consuming...
parfor i = 1:length(steps_hp{iservo, idist})
% for i = 1:length(steps_hp{iservo, idist})
hp = round(xyz_psi2);
% hp(1,:) = hp(1,:)+ steps(i);
% hp_4d = [hp; ones(1,size(hp,2))];
% hp_2d = A*hp_4d;
% hp_2d = hp_2d(1:2,:)';
% num_points(i) = sum(ismember(intersect_2d, hp_2d,'rows'));
hp(1,:) = hp(1,:)+ steps_hp{iservo, idist}(i);
num_points(i) = sum(ismember(intersect_pix, hp','rows'));
end
h1 = figure('WindowStyle','normal','units','normalized','outerposition',[0.5 0.3 0.5 0.5]); plot(steps_hp{iservo, idist},num_points(:), 'k-', 'LineWidth', 3), xlabel('translocation (pix)'), ylabel('# intersection')
answer = MFquestdlg([0.5, 0.3], 'Does the result look correct?', 'Touch hyperplane peaks', 'Yes', 'No', 'Yes');
peak_answer = 'Yes';
while(strcmp(peak_answer,'Yes'))
if strcmp(answer,'Yes')
while true
datacursormode(h1,'on')
hp_peak_ans = inputdlg({'Left peak','Right peak'},'What are the peak points?',1,{'',''},optionsFin);
if ~isempty(hp_peak_ans) && ~isempty(hp_peak_ans{1}) && ~isempty(hp_peak_ans{2})
break
end
end
hp_peaks{iservo, idist} = [str2double(hp_peak_ans{1}) str2double(hp_peak_ans{2})];
% Final confirmation
% project the peak hyperplanes and all coordinates onto psi1 psi2 view
%%
h2 = figure('units','normalized','outerposition',[0 0 1 1]);
th_4d1 = [xyz_psi2(1,:) + hp_peaks{iservo, idist}(1);xyz_psi2(2:3,:);ones(1,size(xyz_psi2,2))];
th_2d1 = A*th_4d1;
th_2d1 = unique(th_2d1(1:2,:)','rows');
th_4d2 = [xyz_psi2(1,:) + hp_peaks{iservo, idist}(2);xyz_psi2(2:3,:);ones(1,size(xyz_psi2,2))];
th_2d2 = A*th_4d2;
th_2d2 = unique(th_2d2(1:2,:)','rows');
scatter(intersect_2d(:,1),intersect_2d(:,2),'k.'), hold on, scatter(th_2d1(:,1), th_2d1(:,2),'r.'), scatter(th_2d2(:,1), th_2d2(:,2),'r.')
%%
answer2 = MFquestdlg([0.5, 0.3], 'Is the result REALLY correct?', 'Final Confirmation', 'Yes', 'No', 'Yes');
switch answer2
case 'Yes'
close(h2);
peak_answer = 'No'; % get out of peak while
hp_decision = 'Yes';
done_flag = 0; % the whole precedure is finally done. get out of while(done_flag) loop.
case 'No'
answer3 = MFquestdlg([0.5, 0.3], 'Do you want to change the steps?', 'Touch hyperplane sweep steps', 'Yes', 'No', 'Yes');
switch answer3
case 'Yes'
step_boundary_cell = inputdlg({'First step','Last step'},'What are the sweep boundaries?',1,{'',''},options);
steps_hp{iservo, idist} = str2double(step_boundary_cell{1}):str2double(step_boundary_cell{2});
peak_answer = 'No'; % get out of peak while
close all
case 'No'
peak_answer = MFquestdlg([0.5, 0.3], 'Do you want to change the peak points?', 'Touch hyperplane peaks', 'Yes', 'No', 'Yes');
if strcmp(peak_answer,'Yes')
close(h2);
else
psi1_return_answer = MFquestdlg([0.5, 0.3], 'Do you want to draw region for psi1?', 'Return to psi1 region selection', 'Yes', 'No', 'Yes');
if strcmp(psi1_return_answer, 'Yes')
close all
psi1_polygon_answer = 'No';
peak_answer = 'No';
hp_decision = 'Yes';
else
psi1_adjust = inputdlg('How much change in psi1? (Negative for CW)', 'psi1 manual adjustment', 1, {''}, options);
if psi1_adjust{1}
psi1(iservo,idist) = psi1(iservo,idist) + str2double(psi1_adjust{1});
else
error(['No optimal hyperplanes peaks found in ' sessionName ' of ' mouseName])
end
end
end
end
end
else % answer = 'No' to question 'Does the result look correct?'
answer3 = MFquestdlg([0.5, 0.3], 'Do you want to change the steps?', 'Touch hyperplane sweep steps', 'Yes', 'No', 'Yes');
switch answer3
case 'Yes'
step_boundary_cell = inputdlg({'First step','Last step'},'What are the sweep boundaries?',1,{'',''},options);
steps_hp{iservo, idist} = str2double(step_boundary_cell{1}):str2double(step_boundary_cell{2});
peak_answer = 'No';
answer7 = 'No';
close all
case 'No'
answer7 = MFquestdlg([0.5, 0.3], 'Do you want to flip psi2?', 'Wierd psi2 error', 'Yes', 'No', 'Yes');
switch answer7
case 'Yes'
psi2Flip = 1-psi2Flip;
psi2(iservo, idist) = -psi2(iservo, idist);
peak_answer = 'No';
case 'No'
% questTitle = 'Return to psi1 polygon';
% start(timer('StartDelay',1,'TimerFcn',@(o,e)set(findall(0,'Tag',questTitle),'WindowStyle','normal')));
psi1_return_answer = MFquestdlg([0.5, 0.3], 'Do you want to draw a region for psi1?', 'Return to psi1 region selection', 'Yes', 'No', 'Yes');
if strcmp(psi1_return_answer, 'Yes')
close all
psi1_polygon_answer = 'No';
peak_answer = 'No';
hp_decision = 'Yes';
else
psi1_adjust = inputdlg('How much change in psi1?', 'psi1 manual adjustment', 1, {''}, options);
if psi1_adjust{1}
psi1(iservo,idist) = psi1(iservo,idist) + str2double(psi1_adjust{1});
else
error(['No optimal hyperplanes peaks found in ' sessionName ' of ' mouseName])
end
end
end
end
end
end
end
end
end
end
%% save parameters
close all
num_points_in_hp{iservo, idist} = num_points;
touch_hp{iservo, idist} = xyz_psi2; % Don't round them! (at least at this saving process)
th_2d = [th_2d1; th_2d2];
cvh = convhull(th_2d);
thPolygon{iservo, idist} = th_2d(cvh,:);
fprintf('%s %s trial type #%d/%d processed\n',mouseName, sessionName, (iservo-1)*length(distance_values) + idist, length(servo_values) * length(distance_values))
end
end
%%
save(savefn,'touch_hp','num_points_in_hp','steps_hp','hp_peaks', 'psi1', 'psi2', 'servo_distance_pair', 'thPolygon')
fprintf('%s %s hp_peaks saved\n', mouseName, sessionName)