diff --git a/repo2docker/buildpacks/conda/environment.py-3.12-linux-64.lock b/repo2docker/buildpacks/conda/environment.py-3.12-linux-64.lock
new file mode 100644
index 000000000..57d613bc6
--- /dev/null
+++ b/repo2docker/buildpacks/conda/environment.py-3.12-linux-64.lock
@@ -0,0 +1,152 @@
+# AUTO GENERATED FROM environment.py-3.12.yml, DO NOT MANUALLY MODIFY
+# Frozen on 2024-05-21 07:52:44 UTC
+# Generated by conda-lock.
+# platform: linux-64
+# input_hash: 368139795b37f28a775052c0b2270e0850ebb567402945c13fbab8dc6355cc7b
+@EXPLICIT
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diff --git a/repo2docker/buildpacks/conda/environment.py-3.12-linux-aarch64.lock b/repo2docker/buildpacks/conda/environment.py-3.12-linux-aarch64.lock
new file mode 100644
index 000000000..26a5084ce
--- /dev/null
+++ b/repo2docker/buildpacks/conda/environment.py-3.12-linux-aarch64.lock
@@ -0,0 +1,151 @@
+# AUTO GENERATED FROM environment.py-3.12.yml, DO NOT MANUALLY MODIFY
+# Frozen on 2024-05-21 07:52:51 UTC
+# Generated by conda-lock.
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+# input_hash: 491fc7a1f5c9466e7bd91af66c076976c6b35633496e179564141fdb96a7c34a
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+https://conda.anaconda.org/conda-forge/noarch/jupyterhub-singleuser-3.1.1-pyh2a2186d_0.conda#6d0a3394efe9ff7c0ad1ed03a6ca3720
diff --git a/repo2docker/buildpacks/conda/environment.py-3.12.yml b/repo2docker/buildpacks/conda/environment.py-3.12.yml
new file mode 100644
index 000000000..323a83f50
--- /dev/null
+++ b/repo2docker/buildpacks/conda/environment.py-3.12.yml
@@ -0,0 +1,15 @@
+# AUTO GENERATED FROM environment.yml, DO NOT MANUALLY MODIFY
+# Generated on 2024-05-21 07:52:44 UTC
+channels:
+- conda-forge
+dependencies:
+- python=3.12.*
+- nodejs=18
+- pip
+- ipywidgets==8.*     # https://github.com/jupyter-widgets/ipywidgets
+- jupyter-offlinenotebook==0.2.*     # https://github.com/manics/jupyter-offlinenotebook
+- jupyter-resource-usage==0.7.*     # https://github.com/jupyter-server/jupyter-resource-usage
+- jupyter_server==1.*     # https://github.com/jupyter-server/jupyter_server
+- jupyterhub-singleuser==3.*     # https://github.com/jupyterhub/jupyterhub
+- jupyterlab==3.*     # https://github.com/jupyterlab/jupyterlab
+- notebook==6.*     # https://github.com/jupyter/notebook
diff --git a/repo2docker/buildpacks/conda/freeze.py b/repo2docker/buildpacks/conda/freeze.py
index a3a18b2b0..aa1fed222 100755
--- a/repo2docker/buildpacks/conda/freeze.py
+++ b/repo2docker/buildpacks/conda/freeze.py
@@ -113,7 +113,7 @@ def set_python(py_env_file, py):
         "py",
         nargs="*",
         help="Python version(s) to update and freeze",
-        default=("3.7", "3.8", "3.9", "3.10", "3.11"),
+        default=("3.7", "3.8", "3.9", "3.10", "3.11", "3.12"),
     )
     parser.add_argument(
         "platform",
diff --git a/tests/conda/py312/environment.yml b/tests/conda/py312/environment.yml
new file mode 100644
index 000000000..94af7b1e3
--- /dev/null
+++ b/tests/conda/py312/environment.yml
@@ -0,0 +1,2 @@
+dependencies:
+  - python=3.12
diff --git a/tests/conda/py312/verify b/tests/conda/py312/verify
new file mode 100755
index 000000000..bc347d686
--- /dev/null
+++ b/tests/conda/py312/verify
@@ -0,0 +1,4 @@
+#!/usr/bin/env python
+import sys
+
+assert sys.version_info[:2] == (3, 12), sys.version