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RagTag error: "OSError: error when opening file" #187

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ylzou867 opened this issue Sep 4, 2024 · 0 comments
Open

RagTag error: "OSError: error when opening file" #187

ylzou867 opened this issue Sep 4, 2024 · 0 comments

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@ylzou867
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ylzou867 commented Sep 4, 2024

I am running RagTag to scaffold an assembly.
It runs fine, makes the log file and part of the output, but at the last step gives an error:

Traceback (most recent call last):
File "/home/zyl/miniforge3/bin/ragtag_patch.py", line 518, in
main()
File "/home/zyl/miniforge3/bin/ragtag_patch.py", line 353, in main
ref_fai = pysam.FastaFile(reference_ctg_fn)
File "pysam/libcfaidx.pyx", line 121, in pysam.libcfaidx.FastaFile.cinit
File "pysam/libcfaidx.pyx", line 181, in pysam.libcfaidx.FastaFile._open
OSError: error when opening file /home/zyl/ragtag/Mjof/ragtag_output/ragtag.patch.ctg.fasta

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