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phame.ctl
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phame.ctl
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refdir = ref # directory where reference files are located
workdir = workdir # directory where contigs/reads files are located and output is stored
reference = 2 # 0:pick a random reference; 1:use given reference; 2: use ANI based reference
reffile = KJ660347.fasta # reference filename
project = ecoli # main alignment file name
cdsSNPS = 0 # 0:no cds SNPS; 1:cds SNPs
buildSNPdb = 0 # 0: only align to reference 1: build SNP database of all complete genome
SNPsfilter = 0.6 # threshold to call SNPs
FirstTime = 1 # 1:yes; 2:update existing SNP alignment
data = 0 # *See below 0:only complete(F); 1:only contig(C); 2:only reads(R);
# 3:combination F+C; 4:combination F+R; 5:combination C+R;
# 6:combination F+C+R; 7:realignment *See below
reads = 2 # 1: single reads; 2: paired reads; 3: both types present;
aligner = bowtie # support bowtie/bwa/minimap2
tree = 0 # 0:no tree; 1:use FastTree; 2:use RAxML; 3:IQ-TREE; 4: use all
bootstrap = 0 # 0:no; 1:yes; # Run bootstrapping *See below
N = 100 # Number of bootstraps to run *See below
PosSelect = 0 # 0:No; 1:use PAML; 2:use HyPhy; 3:use both
code = 1 # 0:Bacteria; 1:Virus
clean = 0 # 0:no clean; 1:clean
threads = 2 # Number of threads to use
cutoff = 0.1 # Linear alignment (LA) coverage against reference - ignores SNPs from organism that have lower cutoff.
* When using data option 1,2,5 need a complete reference to align/map to.
* Use data option 7 when need to extract SNPs using a sublist of already aligned genomes.