Let me get this straight about 'eligible territory'

[a00101]

Thanks. for your reply before.
But still i don't understand exactly for making coverage.

First of all, I know which tool to use to create the coverage.

But what I still don't know is whether I can define the GRCh38 genome file as eligible territory. In other words, should GRCh38 gtf be converted to bed to bigwig?

Or, read coverage varies from sample to sample, so I have to use different coverage files for each sample.

I'm very thank you for your kindness.