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Megalodon v2.5.0 rerio all-context model fails #68

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Olinewms opened this issue Nov 4, 2024 · 0 comments
Open

Megalodon v2.5.0 rerio all-context model fails #68

Olinewms opened this issue Nov 4, 2024 · 0 comments

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@Olinewms
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Olinewms commented Nov 4, 2024

I have been trying to run the res_dna_r941_min_modbases-all-context_v001.cfg basecalling model on some R9 ONT data I generated in 2023. I have Megalodon v2.5.0 and Guppy Version 6.5.7+ca6d6af installed. My command looks like:

megalodon /home/newol210/testdir_purple_Rl_fast5s/ --guppy-server-path /full_path_to_ont-guppy/bin/guppy_basecall_server --guppy-params "-d /full_path__to__rerio_basecall_models/" --guppy-config /full_path__to_rerio_basecall_models/res_dna_r941_min_modbases-all-context_v001.cfg

The output I always receive is:

[15:53:11] Running Megalodon version 2.5.0
[15:53:11] Loading guppy basecalling backend
[guppy/error] basecall_service::BasecallClient::worker_loop: Connection error. [basecall_config_unavailable] could not retrieve details for config from server: /projects/health_sciences/bms/biochemistry/macknight_lab/Oliver/rerio/basecall_models/res_dna_r941_min_modbases-all-context_v001


ERROR: Error connecting to Guppy server. Undefined error: "Basecalling config unavailable: <result.basecall_config_unavailable: 6>"

I would greatly appreciate some help resolving this issue. Thanks in advance.

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