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Problems in starting the analysis. Please help me #423

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Francinepiubeli opened this issue Feb 20, 2023 · 1 comment
Open

Problems in starting the analysis. Please help me #423

Francinepiubeli opened this issue Feb 20, 2023 · 1 comment

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@Francinepiubeli
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Hello everyone, I am new to the world of nanopore sequencing. I've been reading the forum for two weeks and looking for information, maybe it's silly but could someone explain to me when I should do it annotate_raw_with_fastq?

I have my folder with several fasta5 files (FAR64728_pass_barcode07_0d909433_0.fast5) of my problem sample and another one of the genome, but it is also in fasta5, is that the one I should use as reference.fasta?

Thank you for your help

@Francinepiubeli
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This is the error I get

(python2) francineamaral@x86_64-apple-darwin13 tombotest % tombo resquiggle ./fasta5s/ Users/METILOMA/WGA
[21:26:20] Loading minimap2 reference.
******************** ERROR ********************
Failed to load reference genome FASTA for mapping.
(python2) francineamaral@x86_64-apple-darwin13 tombotest %

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