Will this affect my next analysis

[lanyunxin]

Will this affect my next analysis

[lanyunxin]

Unexpected errors occured. See full error stack traces for first (up to) 50 errors in "unexpected_tombo_errors.1679.err"
[20:59:20] Final unsuccessful reads summary (28.7% reads unsuccessfully processed; 432094 total reads):
19.4% ( 291445 reads) : Read event to sequence alignment extends beyond bandwidth
5.7% ( 85932 reads) : Poor raw to expected signal matching (revert with tombo filter clear_filters)
3.6% ( 54639 reads) : Alignment not produced
0.0% ( 23 reads) : Not enough raw signal around potential genomic deletion(s)
0.0% ( 17 reads) : Too much raw signal for mapped sequence
0.0% ( 14 reads) : Fewer changepoints found than requested
0.0% ( 9 reads) : Read failed sequence-based signal re-scaling parameter estimation.
0.0% ( 7 reads) : Unexpected error
0.0% ( 7 reads) : Read contains too many potential genomic deletions
0.0% ( 1 reads) : Base calls not found in FAST5 (see tombo preprocess)
[20:59:20] Saving Tombo reads index to file.

[marcus1487]

Tombo is deprecated. I would highly recommend moving your pipeline to the Remora signal analysis API.