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pipeline does not generate cnv models #15

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egenomics opened this issue Jan 23, 2024 · 1 comment
Open

pipeline does not generate cnv models #15

egenomics opened this issue Jan 23, 2024 · 1 comment
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bug Something isn't working

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@egenomics
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egenomics commented Jan 23, 2024

Description of the bug

The pipeline ends with error with ~120 samples:
A USER ERROR has occurred: Bad input: Filtering removed all intervals. Select less strict filtering criteria.

However it does not generate cnv model or ploidy (a run in cohort mode with the samples provided). Only readcounts are available in folder readcounts (hdf5 format).

Command used and terminal output

nextflow run /playground/nf-core-createpanelrefs_dev/dev \
   -profile singularity \
   --input /playground/dataset_cnvs/samplesheets_sarek/samplesheet_bams_illumina.csv \
   --tools germlinecnvcaller \
   --fasta /corebm/tmp/bams/hg38.fa --dict /corebm/tmp/bams/hg38.dict --fai /corebm/tmp/bams/hg38.fa.fai \
   --outdir /playground/dataset_cnvs/germlinecnvcaller_illumina_reference_nochrM \
   --ploidy_priors /playground/dataset_cnvs/priors.table \
   --padding 250 \
   --bin_length 0 \
   --mappable_regions /playground/dataset_cnvs/Illumina-truseq-rapid-exome_v1.2_hg38_target_orig_nochrM.bed

Relevant files

next.log

System information

nextflow version 23.10.1.5891
Workstation
local executor
singularity
ubuntu 22.04
nf-core/createpanelrefs dev (22/01/2024)

@egenomics egenomics added the bug Something isn't working label Jan 23, 2024
@egenomics egenomics reopened this Jan 24, 2024
@ramprasadn
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Hi @egenomics Could you run the latest dev and see if it works now?

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