i have run the nanoseq pipeline and it is giving me this error, although i have run jaffal seperatly and it worked fine. i dont know why this issue occurrs while running the nanoseq pipeline.

[usama-khan1998]

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| Starting Pipeline at 2025-01-28 05:38 |
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========================================= Stage run_check ==========================================
Running JAFFA version 2.4_dev
Checking for required data files...
JAFFA-version-2.3/hg38_genCode22.fa
JAFFA-version-2.3/hg38_genCode22.tab
/home/ngs/work/44/107fb4f3651d4d0150dbc7afaaa193/JAFFA-version-2.3/known_fusions.txt
JAFFA-version-2.3/hg38.fa
JAFFA-version-2.3/Masked_hg38.1.bt2
JAFFA-version-2.3/hg38_genCode22.1.bt2
All looking good

===================================== Stage get_fasta (sample) =====================================
java -ea -Xmx200m -cp /home/ngs/apps/JAFFA-version-2.3/JAFFA-version-2.3/tools/bbmap/current/ jgi.ReformatReads ignorebadquality=t in=sample.fastq.gz out=sample.fastq/sample.fastq.fasta threads=16
Executing jgi.ReformatReads [ignorebadquality=t, in=sample.fastq.gz, out=sample.fastq/sample.fastq.fasta, threads=16]

Set threads to 16
Input is being processed as unpaired
Input: 156255 reads 59480716 bases
Output: 156255 reads (100.00%) 59480716 bases (100.00%)

Time: 0.826 seconds.
Reads Processed: 156k 189.13k reads/sec
Bases Processed: 59480k 72.00m bases/sec

============================== Stage minimap2_transcriptome (sample) ===============================
/home/ngs/apps/JAFFA-version-2.3/JAFFA-version-2.3/tools/bin/minimap2: error while loading shared libraries: libz.so.1: cannot open shared object file: No such file or directory
Cleaned up file sample.fastq/sample.fastq.paf to .bpipe/trash/sample.fastq.paf
ERROR: stage minimap2_transcriptome failed: Command in stage minimap2_transcriptome failed with exit status = 127 :

/home/ngs/apps/JAFFA-version-2.3/JAFFA-version-2.3/tools/bin/minimap2 -t 16 -x map-ont -c JAFFA-version-2.3/hg38_genCode22.fa sample.fastq/sample.fastq.fasta > sample.fastq/sample.fastq.paf ;

========================================= Pipeline Failed ==========================================

In stage Unknown: One or more parallel stages aborted. The following messages were reported:

---------------------------- minimap2_transcriptome ( sample.fastq ) -----------------------------

Command in stage minimap2_transcriptome failed with exit status = 127 :

/home/ngs/apps/JAFFA-version-2.3/JAFFA-version-2.3/tools/bin/minimap2 -t 16 -x map-ont -c JAFFA-version-2.3/hg38_genCode22.fa sample.fastq/sample.fastq.fasta > sample.fastq/sample.fastq.paf ;

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Use 'bpipe errors' to see output from failed commands.