You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
STAR command fails to produce output in linux/amd64 mode on macOS arm64/v8 in docker profile
Command used and terminal output
% nextflow run nf-core/rnaseq -profile rnaseq,docker -process.containerOptions "--platform linux/amd64"
...
Execution cancelled -- Finishing pending tasks before exit
-[nf-core/rnaseq] Pipeline completed with errors-
ERROR ~ Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (RAP1_IAA_30M_REP1)'
Caused by:
Missing output file(s) *Log.final.out expected by process NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (RAP1_IAA_30M_REP1)
Command executed:
STAR
--genomeDir star
--readFilesIn input1/RAP1_IAA_30M_REP1_primary_1.fastq.gz input2/RAP1_IAA_30M_REP1_primary_2.fastq.gz
--runThreadN 4
--outFileNamePrefix RAP1_IAA_30M_REP1.
if [ -f RAP1_IAA_30M_REP1.Unmapped.out.mate1 ]; then
mv RAP1_IAA_30M_REP1.Unmapped.out.mate1 RAP1_IAA_30M_REP1.unmapped_1.fastq
gzip RAP1_IAA_30M_REP1.unmapped_1.fastq
fi
if [ -f RAP1_IAA_30M_REP1.Unmapped.out.mate2 ]; then
mv RAP1_IAA_30M_REP1.Unmapped.out.mate2 RAP1_IAA_30M_REP1.unmapped_2.fastq
gzip RAP1_IAA_30M_REP1.unmapped_2.fastq
fi
Version: 24.10.0 build 5928
Created: 27-10-2024 18:36 UTC (14:36 EDT)
System: Mac OS X 15.1
Runtime: Groovy 4.0.23 on OpenJDK 64-Bit Server VM 11.0.13+7-b1751.21
Encoding: UTF-8 (UTF-8)
Description of the bug
STAR command fails to produce output in linux/amd64 mode on macOS arm64/v8 in docker profile
Command used and terminal output
% nextflow run nf-core/rnaseq -profile rnaseq,docker -process.containerOptions "--platform linux/amd64"
...
Execution cancelled -- Finishing pending tasks before exit
-[nf-core/rnaseq] Pipeline completed with errors-
ERROR ~ Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (RAP1_IAA_30M_REP1)'
Caused by:
Missing output file(s)
*Log.final.out
expected by processNFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (RAP1_IAA_30M_REP1)
Command executed:
STAR
--genomeDir star
--readFilesIn input1/RAP1_IAA_30M_REP1_primary_1.fastq.gz input2/RAP1_IAA_30M_REP1_primary_2.fastq.gz
--runThreadN 4
--outFileNamePrefix RAP1_IAA_30M_REP1.
--sjdbGTFfile genome_gfp.gtf
--outSAMattrRGline 'ID:RAP1_IAA_30M_REP1' 'SM:RAP1_IAA_30M_REP1'
--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --outSAMstrandField intronMotif --quantTranscriptomeSAMoutput BanSingleEnd
if [ -f RAP1_IAA_30M_REP1.Unmapped.out.mate1 ]; then
mv RAP1_IAA_30M_REP1.Unmapped.out.mate1 RAP1_IAA_30M_REP1.unmapped_1.fastq
gzip RAP1_IAA_30M_REP1.unmapped_1.fastq
fi
if [ -f RAP1_IAA_30M_REP1.Unmapped.out.mate2 ]; then
mv RAP1_IAA_30M_REP1.Unmapped.out.mate2 RAP1_IAA_30M_REP1.unmapped_2.fastq
gzip RAP1_IAA_30M_REP1.unmapped_2.fastq
fi
cat <<-END_VERSIONS > versions.yml$(echo $ (samtools --version 2>&1) | sed 's/^.samtools //; s/Using.$//')$(echo $ (gawk --version 2>&1) | sed 's/^.GNU Awk //; s/, .$//')
"NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN":
star: $(STAR --version | sed -e "s/STAR_//g")
samtools:
gawk:
END_VERSIONS
Command exit status:
0
Command output:
(empty)
Work dir:
/Users/pikalaxalt/nextflow-launch-directories/work/d2/c757228cbe86dafe7412bc3d1afd2d
Container:
quay.io/nf-core/star_samtools_htslib_gawk:10c6e8c834460019
Tip: when you have fixed the problem you can continue the execution adding the option
-resume
to the run command line-- Check '.nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting
-- Check '.nextflow.log' file for details
Relevant files
Version: 24.10.0 build 5928
Created: 27-10-2024 18:36 UTC (14:36 EDT)
System: Mac OS X 15.1
Runtime: Groovy 4.0.23 on OpenJDK 64-Bit Server VM 11.0.13+7-b1751.21
Encoding: UTF-8 (UTF-8)
Core Nextflow options
revision : master
runName : serene_bhabha
containerEngine : docker
launchDir : /Users/pikalaxalt/nextflow-launch-directories
workDir : /Users/pikalaxalt/nextflow-launch-directories/work
projectDir : /Users/pikalaxalt/.nextflow/assets/nf-core/rnaseq
userName : pikalaxalt
profile : docker,test
configFiles :
nextflow version: 24.10.0
hardware: macbook
executor: local
container engine: docker
os: macos 15.1 silicon (arm64 v8)
version of nf-core/rnaseq: 00f924c
System information
No response
The text was updated successfully, but these errors were encountered: