diff --git a/tests/drivers/uart/uart_mix_fifo_poll/boards/nrf5340bsim_nrf5340_cpuapp.overlay b/tests/drivers/uart/uart_mix_fifo_poll/boards/nrf5340bsim_nrf5340_cpuapp.overlay
new file mode 100644
index 00000000000..bfd4f8676f1
--- /dev/null
+++ b/tests/drivers/uart/uart_mix_fifo_poll/boards/nrf5340bsim_nrf5340_cpuapp.overlay
@@ -0,0 +1,3 @@
+/* SPDX-License-Identifier: Apache-2.0 */
+
+#include "nrf5340dk_nrf5340_cpuapp.overlay"
diff --git a/tests/drivers/uart/uart_mix_fifo_poll/testcase.yaml b/tests/drivers/uart/uart_mix_fifo_poll/testcase.yaml
index 1b13bc57166..91470dc990b 100644
--- a/tests/drivers/uart/uart_mix_fifo_poll/testcase.yaml
+++ b/tests/drivers/uart/uart_mix_fifo_poll/testcase.yaml
@@ -8,6 +8,7 @@ common:
     - nrf52840dk/nrf52840
     - nrf9160dk/nrf9160
     - nrf5340dk/nrf5340/cpuapp
+    - nrf5340bsim/nrf5340/cpuapp
     - nrf54l15dk/nrf54l15/cpuapp
     - nrf54h20dk/nrf54h20/cpuapp
     - nrf54h20dk/nrf54h20/cpurad