diff --git a/docs/iso-sens.jl b/docs/iso-sens.jl index de97b31..15bb8ce 100644 --- a/docs/iso-sens.jl +++ b/docs/iso-sens.jl @@ -62,7 +62,7 @@ pkac1_coef = coef(pkac1_fit) #--- println("PKACI") println("Basal activity: ", pkac1_coef[3]) -println("Maximal activity: ", pkac1_coef[1] + pkac1_coef[3]) +println("Activated activity: ", pkac1_coef[1]) println("Michaelis constant: ", pkac1_coef[2], " μM") println("RMSE: ", rmse(pkac1_fit)) @@ -83,7 +83,7 @@ pkac2_coef = coef(pkac2_fit) #--- println("PKACII") println("Basal activity: ", pkac2_coef[3]) -println("Maximal activity: ", pkac2_coef[1] + pkac2_coef[3]) +println("Activated activity: ", pkac2_coef[1]) println("Michaelis constant: ", pkac2_coef[2], " μM") println("RMSE: ", rmse(pkac2_fit)) @@ -104,7 +104,7 @@ pp1_coef = coef(pp1_fit) #--- println("PP1") -println("Basal activity: ", pp1_coef[1]) +println("Repressible activity: ", pp1_coef[1]) println("Minimal activity: ", pp1_coef[3]) println("Repressive Michaelis constant: ", pp1_coef[2], " μM") println("RMSE: ", rmse(pp1_fit)) @@ -146,11 +146,11 @@ function plbp_analytic(iso) k_PP1_PLB = 8.5Hz / μM Km_PP1_PLB = 7.0μM PKACI_basal = 0.0734 ## basal activity - PKACI_activated = 0.2728 + PKACI_activated = 0.1995 PKACI_KM = 0.0139μM PP1_basal = 0.8927 - PP1_activated = 0.1840 - PP1_KI = 0.01026μM + PP1_activated = 0.0492 + PP1_KI = 0.00637μM ## Solve for Vf * x / (x + k1) = Vr * (1 - x) / (1 - x + k2) PKACI = PKACItot * (PKACI_basal + PKACI_activated * hil(iso, PKACI_KM)) diff --git a/src/isoproterenol.jl b/src/isoproterenol.jl index aa194e4..b2f8d12 100644 --- a/src/isoproterenol.jl +++ b/src/isoproterenol.jl @@ -311,14 +311,14 @@ function get_bar_sys_reduced(ISO=0μM; name=:bar_sys) Km_pp2a_RyR = 4.1μM ## Fitted PKACI, PKACII, and PP1 parameters PKACI_basal = 0.0734 ## basal activity - PKACI_activated = 0.2728 + PKACI_activated = 0.1995 PKACI_KM = 0.0139μM PKACII_basal = 0.1840 ## basal activity - PKACII_activated = 0.5285 + PKACII_activated = 0.3444 PKACII_KM = 0.0103μM PP1_basal = 0.8927 - PP1_activated = 0.1840 - PP1_KI = 0.01026μM + PP1_activated = 0.0492 + PP1_KI = 0.00637μM end vs = @variables begin