diff --git a/test/data/NF000185.2.HMM b/test/data/NF000185.2.HMM new file mode 100644 index 00000000..8096d9b5 --- /dev/null +++ b/test/data/NF000185.2.HMM @@ -0,0 +1,1158 @@ +HMMER3/f [3.1b2 | February 2015] +NAME BlaEC +ACC NF000185.2 +DESC NCBIFAM: BlaEC family class C beta-lactamase +LENG 377 +ALPH amino +RF no +MM no +CONS yes +CS no +MAP yes +DATE Tue Aug 27 12:36:06 2019 +NSEQ 10 +EFFN 0.395508 +CKSUM 2497064882 +GA 850 850 +TC 850 850 +NC 750 750 +STATS LOCAL MSV -11.2521 0.69956 +STATS LOCAL VITERBI -12.4431 0.69956 +STATS LOCAL FORWARD -5.8197 0.69956 +HMM A C D E F G H I K L M N P Q R S T V W Y + m->m m->i m->d i->m i->i d->m d->d + COMPO 2.41209 4.33506 3.04628 2.87324 3.31941 2.70889 3.85702 2.77744 2.76760 2.43608 3.70783 3.12524 3.11488 3.09890 3.07189 2.69485 2.79946 2.61865 4.05040 3.38712 + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.00000 * + 1 2.90739 4.47013 3.96211 3.55124 3.17646 3.77266 4.30552 2.34405 3.32305 1.78541 1.54528 3.83830 4.23451 3.71145 3.53765 3.24703 3.21198 2.34011 5.00517 3.75855 1 m - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 2 3.15688 4.58374 4.12510 3.82588 1.15666 3.86210 3.84636 2.69288 3.73540 2.08313 3.43676 3.92776 4.32413 3.92674 3.87541 3.42982 3.45880 2.67712 4.12350 2.50233 2 f - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 3 2.89996 4.85593 3.07027 2.71008 4.22349 3.39591 3.71939 3.64811 1.15703 3.23238 4.20042 3.13740 3.90388 2.91717 2.42428 2.94863 3.16052 3.36129 5.31375 4.11544 3 k - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 4 2.45845 4.24798 3.50934 3.10918 3.64950 3.28061 4.07147 2.39989 2.97899 2.51223 3.61358 3.38601 3.88107 3.37525 3.26959 2.69303 1.70464 2.35993 5.21696 3.95063 4 t - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 5 2.37253 4.21665 3.35891 3.10979 3.89986 3.05793 4.13288 3.01714 3.05109 2.88342 3.94119 3.33087 3.75604 3.45066 3.31239 2.57313 1.31946 2.72096 5.34509 4.11716 5 t - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 6 3.06499 4.55292 4.05625 3.69808 3.10822 3.87549 4.37965 2.31070 3.46653 0.98368 3.16114 3.98122 4.32660 3.85467 3.65512 3.44008 3.36392 2.32622 4.95535 3.65896 6 l - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 7 2.49977 1.12381 3.99662 3.71922 3.73155 3.13811 4.38463 2.86361 3.54623 2.78188 3.90143 3.72816 3.83854 3.89599 3.68446 2.77089 2.95995 2.61330 5.16285 4.01922 7 c - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 8 1.40868 4.08888 3.38785 3.10302 4.00459 2.94258 4.13447 3.05670 3.08910 2.97815 3.94074 3.26882 3.66739 3.42716 3.37216 2.40342 2.27129 2.69832 5.44561 4.22724 8 a - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 9 3.06499 4.55292 4.05625 3.69808 3.10822 3.87549 4.37965 2.31070 3.46653 0.98368 3.16114 3.98122 4.32660 3.85467 3.65512 3.44008 3.36392 2.32622 4.95535 3.65896 9 l - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 10 3.06499 4.55292 4.05625 3.69808 3.10822 3.87549 4.37965 2.31070 3.46653 0.98368 3.16114 3.98122 4.32660 3.85467 3.65512 3.44008 3.36392 2.32622 4.95535 3.65896 10 l - 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- - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 17 2.37253 4.21665 3.35891 3.10979 3.89986 3.05793 4.13288 3.01714 3.05109 2.88342 3.94119 3.33087 3.75604 3.45066 3.31239 2.57313 1.31946 2.72096 5.34509 4.11716 17 t - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 18 3.15688 4.58374 4.12510 3.82588 1.15666 3.86210 3.84636 2.69288 3.73540 2.08313 3.43676 3.92776 4.32413 3.92674 3.87541 3.42982 3.45880 2.67712 4.12350 2.50233 18 f - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 19 1.13921 4.15499 3.38209 3.17212 3.97315 2.98118 4.20347 3.08672 3.18629 2.97176 4.01471 3.34159 3.71286 3.53562 3.44111 2.50703 2.77880 2.75557 5.40869 4.20023 19 a - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 20 1.13921 4.15499 3.38209 3.17212 3.97315 2.98118 4.20347 3.08672 3.18629 2.97176 4.01471 3.34159 3.71286 3.53562 3.44111 2.50703 2.77880 2.75557 5.40869 4.20023 20 a - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 21 2.64969 4.41823 3.24841 3.07741 4.07558 3.11050 4.15995 3.60180 3.12801 3.23687 4.28481 3.39129 0.99567 3.51942 3.38105 2.81514 3.08533 3.25638 5.28279 4.19589 21 p - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 22 2.81998 4.79889 2.85340 2.60938 3.95578 3.31407 3.76609 3.57614 2.43658 3.08648 4.11916 3.06743 3.86795 1.38807 2.72135 2.87239 3.11820 3.31172 5.24374 3.93271 22 q - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 23 2.79830 4.92020 2.77401 2.46738 4.18622 3.35855 3.63107 3.65925 2.08029 3.19853 4.11253 2.94784 3.85375 1.72414 2.48208 2.81121 3.04690 3.35320 5.35520 4.05263 23 q - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 24 2.96719 4.41178 4.21419 3.82875 3.32118 3.97377 4.57362 1.18616 3.65507 1.92685 3.27216 4.08569 4.41496 4.01325 3.86117 3.46301 3.26874 1.85068 5.19331 3.92289 24 i - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 25 2.65880 4.64894 2.66979 2.54810 3.99987 3.13150 3.86086 3.69464 2.75885 3.35163 4.30910 1.31252 3.78039 3.13534 3.10908 2.73035 3.03910 3.32520 5.31562 3.95554 25 n - 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- - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 29 2.91178 4.74023 3.05188 2.80569 3.23193 3.38215 1.32847 3.60165 2.65125 3.10516 4.14342 3.21750 3.93132 3.17149 2.92083 2.98385 3.21325 3.33856 4.69776 3.18557 29 h - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 30 2.91647 4.75709 3.39912 2.93046 4.09086 3.41736 3.77146 3.62870 2.21423 3.15980 4.16429 3.29541 3.92589 2.99778 1.11204 3.00587 3.18933 3.35674 5.20857 4.03823 30 r - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 31 2.37253 4.21665 3.35891 3.10979 3.89986 3.05793 4.13288 3.01714 3.05109 2.88342 3.94119 3.33087 3.75604 3.45066 3.31239 2.57313 1.31946 2.72096 5.34509 4.11716 31 t - 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- - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 368 1.13921 4.15499 3.38209 3.17212 3.97315 2.98118 4.20347 3.08672 3.18629 2.97176 4.01471 3.34159 3.71286 3.53562 3.44111 2.50703 2.77880 2.75557 5.40869 4.20023 368 a - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 369 1.13921 4.15499 3.38209 3.17212 3.97315 2.98118 4.20347 3.08672 3.18629 2.97176 4.01471 3.34159 3.71286 3.53562 3.44111 2.50703 2.77880 2.75557 5.40869 4.20023 369 a - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 370 3.20590 4.60870 3.96982 3.65713 2.59177 3.64157 3.80631 3.22790 3.38181 2.63607 3.86532 3.82324 4.15304 3.77394 3.52399 3.41023 3.50264 3.11598 1.06888 2.59855 370 w - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 371 2.81998 4.79889 2.85340 2.60938 3.95578 3.31407 3.76609 3.57614 2.43658 3.08648 4.11916 3.06743 3.86795 1.38807 2.72135 2.87239 3.11820 3.31172 5.24374 3.93271 371 q - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 372 2.96719 4.41178 4.21419 3.82875 3.32118 3.97377 4.57362 1.18616 3.65507 1.92685 3.27216 4.08569 4.41496 4.01325 3.86117 3.46301 3.26874 1.85068 5.19331 3.92289 372 i - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 373 3.06499 4.55292 4.05625 3.69808 3.10822 3.87549 4.37965 2.31070 3.46653 0.98368 3.16114 3.98122 4.32660 3.85467 3.65512 3.44008 3.36392 2.32622 4.95535 3.65896 373 l - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 374 2.65880 4.64894 2.66979 2.54810 3.99987 3.13150 3.86086 3.69464 2.75885 3.35163 4.30910 1.31252 3.78039 3.13534 3.10908 2.73035 3.03910 3.32520 5.31562 3.95554 374 n - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 375 1.13921 4.15499 3.38209 3.17212 3.97315 2.98118 4.20347 3.08672 3.18629 2.97176 4.01471 3.34159 3.71286 3.53562 3.44111 2.50703 2.77880 2.75557 5.40869 4.20023 375 a - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 376 3.06499 4.55292 4.05625 3.69808 3.10822 3.87549 4.37965 2.31070 3.46653 0.98368 3.16114 3.98122 4.32660 3.85467 3.65512 3.44008 3.36392 2.32622 4.95535 3.65896 376 l - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.03413 3.79031 4.51266 0.61958 0.77255 0.48576 0.95510 + 377 2.81998 4.79889 2.85340 2.60938 3.95578 3.31407 3.76609 3.57614 2.43658 3.08648 4.11916 3.06743 3.86795 1.38807 2.72135 2.87239 3.11820 3.31172 5.24374 3.93271 377 q - - - + 2.68618 4.42225 2.77519 2.73123 3.46354 2.40513 3.72494 3.29354 2.67741 2.69355 4.24690 2.90347 2.73739 3.18146 2.89801 2.37887 2.77519 2.98518 4.58477 3.61503 + 0.02310 3.77928 * 0.61958 0.77255 0.00000 * +// diff --git a/test/test_args.py b/test/test_args.py index e2845f67..1715418c 100644 --- a/test/test_args.py +++ b/test/test_args.py @@ -282,6 +282,27 @@ def test_proteins_failiing(parameters, tmpdir): assert proc.returncode != 0 +@pytest.mark.parametrize( + 'parameters', + [ + (['--hmms']), # not provided + (['--hmms', '']), # empty + (['--hmms', 'foo']), # not existing + (['--hmms', 'test/data/empty']), # empty file + (['--hmms', 'test/data/nonsense.txt']) # nonsense file + ] +) +def test_hmms_failiing(parameters, tmpdir): + # test HMM file arguments + proc = run( + ['bin/bakta', '--db', 'test/db', '--output', tmpdir, '--force'] + + parameters + + ['--skip-tmrna', '--skip-trna', '--skip-rrna', '--skip-ncrna', '--skip-ncrna-region', '--skip-crispr', '--skip-sorf', '--skip-ori', '--skip-gap', '--skip-plot'] + + ['test/data/NC_002127.1.fna'] + ) + assert proc.returncode != 0 + + @pytest.mark.parametrize( 'parameters', [ diff --git a/test/test_bakta.py b/test/test_bakta.py index 31297f49..4b241bd1 100644 --- a/test/test_bakta.py +++ b/test/test_bakta.py @@ -93,7 +93,26 @@ def test_bakta_plasmid(tmpdir): ) def test_bakta_genome(db, tmpdir): # full test on complete genome in compliant mode - proc = run(['bin/bakta', '--db', f'test/{db}', '--verbose', '--output', tmpdir, '--force', '--force', '--prefix', 'test', '--min-contig-length', '200', '--complete', '--compliant', '--proteins', 'test/data/user-proteins.faa', '--genus', 'Foo gen. nov.', '--species', 'bar sp. nov.', '--strain', 'test 1', 'test/data/GCF_000008865.2.fna.gz']) + proc = run( + [ + 'bin/bakta', + '--db', f'test/{db}', + '--verbose', + '--output', tmpdir, + '--force', + '--prefix', 'test', + '--min-contig-length', '200', + '--complete', + '--genus', 'Foo gen. nov.', + '--species', 'bar sp. nov.', + '--strain', 'test 1', + # '--replicons', 'test/data/replicons.tsv', + # '--regions', 'test/data/NC_002127.1-region.gff3', + # '--proteins', 'test/data/user-proteins.faa', + '--hmms', 'test/data/NF000185.2.HMM', + '--compliant', + 'test/data/GCF_000008865.2.fna.gz'] + ) assert proc.returncode == 0 tmpdir_path = Path(tmpdir)