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I am not sure if this is a bug or if I am setting up the command incorrectly, but when trying to filter alignments based on the first nucleotide in the query sequence, no match is found. This what I tried (bamtools 2.5.1):
1. Using my actual query, where I am trying to get sequences that start with an "A"
This is a bit puzzling. Could you please help me construct a query to select alignments whose query sequence starts with a particular nucleotide? Along the same lines, and related to this question, what is the best way to take in account the reverse-complemented sequences (0x10 flag)?
The text was updated successfully, but these errors were encountered:
adomingues
changed the title
queryBases filter with patter returns no matching reads
queryBases filter with pattern returns no matching reads
May 6, 2019
I am not sure if this is a bug or if I am setting up the command incorrectly, but when trying to filter alignments based on the first nucleotide in the query sequence, no match is found. This what I tried (bamtools 2.5.1):
1. Using my actual query, where I am trying to get sequences that start with an "A"
This is unexpected because there are plenty of sequences that should match this pattern:
2. testing a (full) sequence that exists in the file
which returns the expected output.
3. testing a subset of the above sequence with and without regex
or with pattern
Which returns the expected result. However, if I restrict it to only a single nucleotide, it again fails to return any match:
This is a bit puzzling. Could you please help me construct a query to select alignments whose query sequence starts with a particular nucleotide? Along the same lines, and related to this question, what is the best way to take in account the reverse-complemented sequences (0x10 flag)?
The text was updated successfully, but these errors were encountered: