Skip to content

Commit

Permalink
add copy again
Browse files Browse the repository at this point in the history
  • Loading branch information
jykr committed Mar 30, 2024
1 parent 217a317 commit 78193f7
Show file tree
Hide file tree
Showing 13 changed files with 23 additions and 21 deletions.
2 changes: 2 additions & 0 deletions .github/workflows/documentation.yml
Original file line number Diff line number Diff line change
Expand Up @@ -19,6 +19,8 @@ jobs:
- name: Build Documentation
working-directory: docs
run: sphinx-build . _build
- name: copy image files
run: cp -r docs/assets docs/_build/assets/
- uses: actions/upload-pages-artifact@v3
with:
name: github-pages
Expand Down
2 changes: 1 addition & 1 deletion docs/count.md
Original file line number Diff line number Diff line change
Expand Up @@ -18,7 +18,7 @@ bean count --R1 R1.fq --R2 R2.fq -b A -f sgRNA_info_table.csv -r
```
By default, `bean count[-samples]` assume R1 and R2 are trimmed off of the adapter sequence. You may need to adjust the command arguments according to your read structure.

<img src="assets/sequence_struct.png" alt="Read structuren" width="600"/>
<img src="/crispr-bean/assets/sequence_struct.png" alt="Read structuren" width="600"/>

See full detail [below](#full-parameters).

Expand Down
2 changes: 1 addition & 1 deletion docs/filter.md
Original file line number Diff line number Diff line change
Expand Up @@ -16,7 +16,7 @@ You may want to adjust the flitering parameters to obtain optimal balance betwee
# Translating alleles
If you want to obtain **amino acid level variant** for coding sequence tiling screens, provide coding sequence positions which variants occuring within the coding sequence will be translated. *This is optional, but **highly recommended** to increase per-(coding)variant support.*

<img src="assets/translation.png" alt="Allele translation" width="500"/>
<img src="/crispr-bean/assets/translation.png" alt="Allele translation" width="500"/>


```bash
Expand Down
4 changes: 2 additions & 2 deletions docs/ldl_cds.md
Original file line number Diff line number Diff line change
Expand Up @@ -4,11 +4,11 @@ Tiling screen that tiles gRNA densely across locus or multiple loci, selected ba
<table>
<tr>
<th>Library design</th>
<td>Tiling (gRNAs tile each locus densely) <br> <img src="assets/tiling.png" alt="tiling library design" width="300"/> </td>
<td>Tiling (gRNAs tile each locus densely) <br> <img src="/crispr-bean/assets/tiling.png" alt="tiling library design" width="300"/> </td>
</tr>
<tr>
<th>Selection</th>
<td>Cells are sorted based on FACS signal quantiles <br> <img src="assets/[email protected]" alt="variant library design" width="300"/></td>
<td>Cells are sorted based on FACS signal quantiles <br> <img src="/crispr-bean//crispr-bean/assets/[email protected]" alt="variant library design" width="300"/></td>
</tr>
</table>

Expand Down
6 changes: 3 additions & 3 deletions docs/ldl_var.md
Original file line number Diff line number Diff line change
@@ -1,14 +1,14 @@
# Variant sorting screen tutorial
## Variant sorting screen tutorial
GWAS variant screen with per-variant gRNA tiling design, selected based on FACS signal quantiles.

<table>
<tr>
<th>Library design</th>
<td>Variant (gRNAs tile each target variant) <br> <img src="assets/variant.png" alt="variant library design" width="600"/></td>
<td>Variant (gRNAs tile each target variant) <br> <img src="/crispr-bean/assets/variant.png" alt="variant library design" width="600"/></td>
</tr>
<tr>
<th>Selection</th>
<td>Cells are sorted based on FACS signal quantiles <br> <img src="assets/[email protected]" alt="variant library design" width="300"/></td>
<td>Cells are sorted based on FACS signal quantiles <br> <img src="/crispr-bean/assets/[email protected]" alt="variant library design" width="300"/></td>
</tr>
</table>

Expand Down
2 changes: 1 addition & 1 deletion docs/profile.md
Original file line number Diff line number Diff line change
Expand Up @@ -5,4 +5,4 @@ bean profile my_sorting_screen.h5ad -o output_prefix `# Prefix for editing profi
# Output
Above command produces `prefix_editing_preference.[html,ipynb]` as editing preferences ([see example](../notebooks/profile_editing_preference.ipynb)).

<img src="assets/profile_output.png" alt="Allele translation" width="700" />
<img src="/crispr-bean/assets/profile_output.png" alt="Allele translation" width="700" />
6 changes: 3 additions & 3 deletions docs/prolif_gwas.md
Original file line number Diff line number Diff line change
@@ -1,14 +1,14 @@
# Variant sorting screen tutorial
## Variant survival screen tutorial
GWAS variant screen with per-variant gRNA tiling design, selected based on FACS signal quantiles.

<table>
<tr>
<th>Library design</th>
<td>Variant (gRNAs tile each target variant) <br> <img src="assets/variant.png" alt="variant library design" width="600"/></td>
<td>Variant (gRNAs tile each target variant) <br> <img src="/crispr-bean/assets/variant.png" alt="variant library design" width="600"/></td>
</tr>
<tr>
<th>Selection</th>
<td>Cells are sorted based on FACS signal quantiles <br> <img src="assets/proliferation.png" alt="variant library design" width="300"/></td>
<td>Cells are sorted based on FACS signal quantiles <br> <img src="/crispr-bean/assets/proliferation.png" alt="variant library design" width="300"/></td>
</tr>
</table>

Expand Down
2 changes: 1 addition & 1 deletion docs/prolif_gwas.rst
Original file line number Diff line number Diff line change
@@ -1,4 +1,4 @@
GWAS variant library
Proliferation screen with GWAS library
***********************
.. mdinclude:: profile_gwas.md

Expand Down
2 changes: 1 addition & 1 deletion docs/qc.md
Original file line number Diff line number Diff line change
Expand Up @@ -17,7 +17,7 @@ bean qc \

`bean qc` supports following quality control and masks samples with low quality. Specifically:

<img src="assets/qc_output.png" alt="Allele translation" width="900"/>
<img src="/crispr-bean/assets/qc_output.png" alt="Allele translation" width="900"/>

* Plots guide coverage and the uniformity of coverage
* Guide count correlation between samples
Expand Down
2 changes: 1 addition & 1 deletion docs/reporterscreen.md
Original file line number Diff line number Diff line change
@@ -1,6 +1,6 @@
BEAN stores mapped gRNA and allele counts in `ReporterScreen` object which is compatible with [AnnData](https://anndata.readthedocs.io/en/latest/index.html).

<img src="assets/data_structure_v2.png" alt="ReporterScreen object structure" width="700" />
<img src="/crispr-bean/assets/data_structure_v2.png" alt="ReporterScreen object structure" width="700" />

* `.guides`: guide information provided in input (`gRNA_library.csv` in above example)
* `.samples`: sample information provided in input (`sample_list.csv` in above example)
Expand Down
4 changes: 2 additions & 2 deletions docs/run.md
Original file line number Diff line number Diff line change
Expand Up @@ -6,7 +6,7 @@ BEAN uses Bayesian network to incorporate gRNA editing outcome to provide poster

For the full detail, see the method section of the [BEAN manuscript](https://www.medrxiv.org/content/10.1101/2023.09.08.23295253v1).

<img src="assets/bean.gif" alt="model" width="700"/>
<img src="/crispr-bean/assets/bean.gif" alt="model" width="700"/>

<br></br>

Expand Down Expand Up @@ -45,7 +45,7 @@ See full list of parameters [below](#full-parameters).
# Output
<img src="assets/model_output.png" alt="model" width="700"/>
<img src="/crispr-bean/assets/model_output.png" alt="model" width="700"/>
Above command produces
* `output_prefix/bean_element_result.[model_type].csv` with following columns:
Expand Down
4 changes: 2 additions & 2 deletions docs/tutorials/ldl_cds.md
Original file line number Diff line number Diff line change
Expand Up @@ -4,11 +4,11 @@ Tiling screen that tiles gRNA densely across locus or multiple loci, selected ba
<table>
<tr>
<th>Library design</th>
<td>Tiling (gRNAs tile each locus densely) <br> <img src="assets/tiling.png" alt="tiling library design" width="300"/> </td>
<td>Tiling (gRNAs tile each locus densely) <br> <img src="/crispr-bean/assets/tiling.png" alt="tiling library design" width="300"/> </td>
</tr>
<tr>
<th>Selection</th>
<td>Cells are sorted based on FACS signal quantiles <br> <img src="assets/[email protected]" alt="variant library design" width="300"/></td>
<td>Cells are sorted based on FACS signal quantiles <br> <img src="/crispr-bean//crispr-bean/assets/[email protected]" alt="variant library design" width="300"/></td>
</tr>
</table>

Expand Down
6 changes: 3 additions & 3 deletions docs/tutorials/ldl_var.md
Original file line number Diff line number Diff line change
@@ -1,14 +1,14 @@
# Variant sorting screen tutorial
## Variant sorting screen tutorial
GWAS variant screen with per-variant gRNA tiling design, selected based on FACS signal quantiles.

<table>
<tr>
<th>Library design</th>
<td>Variant (gRNAs tile each target variant) <br> <img src="assets/variant.png" alt="variant library design" width="600"/></td>
<td>Variant (gRNAs tile each target variant) <br> <img src="/crispr-bean/assets/variant.png" alt="variant library design" width="600"/></td>
</tr>
<tr>
<th>Selection</th>
<td>Cells are sorted based on FACS signal quantiles <br> <img src="assets/[email protected]" alt="variant library design" width="300"/></td>
<td>Cells are sorted based on FACS signal quantiles <br> <img src="/crispr-bean/assets/[email protected]" alt="variant library design" width="300"/></td>
</tr>
</table>

Expand Down

0 comments on commit 78193f7

Please sign in to comment.