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jykr authored Sep 27, 2023
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### Input file format
#### 1. gRNA_library.csv
File should contain following columns. Also see examples for [variant library](crispr-bean/tests/data/test_guide_info.csv) and [tiling library](crispr-bean/tests/data/test_guide_info_tiling.csv).
File should contain following columns.
* `name`: gRNA ID column
* `sequence`: gRNA sequence
* `barcode`: R2 barcode to help match reporter to gRNA
* In order to use accessibility in the [variant effect quantification](#bean-run-quantify-variant-effects), provide accessibility information in one of two options. (For non-targeting guides, provide NA values (empty cell).)
* Option 1: `chr` & `genomic_pos`: Chromosome (ex. `chr19`) and genomic position of guide sequence. You will have to provide the path to the bigwig file with matching reference version in `bean-run`.
* Option 2: `accessibility_signal`: ATAC-seq signal value of the target loci of each guide.
* For variant screen (gRNAs are designed to target specific variants and ignores bystander edits)
* For variant library (gRNAs are designed to target specific variants and ignores bystander edits)
<img src="imgs/variant_screen_gRNA_design.svg" alt="variant screen design" width="500"/>
* `target` : This column denotes which target variant/element of each gRNA. This is not used in `bean-count[-samples]` but required to run `bean-run` in later steps.
* `target_group [Optional]`: If negative/positive control gRNA will be considered in `bean-qc` and/or `bean-run`, specify as "NegCtrl"/"PosCtrl" in this column.
* `target_pos [Optional]`: If `--match_target_pos` flag is used, input file needs `target_pos` which specifies 0-based relative position of targeted base within Reporter sequence.
* For tiling screen (gRNAs tile coding / noncoding sequences)
* For tiling library (gRNAs tile coding / noncoding sequences)
* `strand`: Specifies gRNA strand information relative to the reference genome.
* `chrom`: Chromosome of gRNA targeted locus.
* `start_pos`: gRNA starting position in the genome. Required when you provide `strand` column. Should specify the smaller coordinate value among start and end position regardless of gRNA strandedness.

Also see examples for [variant library](tests/data/test_guide_info.csv) and [tiling library](tests/data/test_guide_info_tiling.csv).

#### 2. sample_list.csv
File should contain following columns with header.
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