From 25fc9f57622fe05527e5826eea956fc3aa3b8a20 Mon Sep 17 00:00:00 2001
From: Manuel Lera-Ramirez <manulera14@gmail.com>
Date: Thu, 25 Apr 2024 15:17:47 +0100
Subject: [PATCH] closes #216 (2)

---
 src/pydna/crispr.py         | 3 ++-
 tests/test_module_crispr.py | 2 ++
 2 files changed, 4 insertions(+), 1 deletion(-)

diff --git a/src/pydna/crispr.py b/src/pydna/crispr.py
index 28d21de3..b3a14ab1 100644
--- a/src/pydna/crispr.py
+++ b/src/pydna/crispr.py
@@ -29,7 +29,8 @@ class _cas(ABC):
     def __init__(self, protospacer):
         self.protospacer = protospacer.upper()
         self.compsite = re.compile(
-            f"(?=(?P<watson>{protospacer}{self.pam}))|(?=(?P<crick>{rc(self.pam)}{rc(protospacer)}))", re.UNICODE
+            f"(?=(?P<watson>{self.protospacer}{self.pam}))|(?=(?P<crick>{rc(self.pam)}{rc(self.protospacer)}))",
+            re.UNICODE,
         )
 
     @abstractmethod
diff --git a/tests/test_module_crispr.py b/tests/test_module_crispr.py
index 62349323..af8c6408 100644
--- a/tests/test_module_crispr.py
+++ b/tests/test_module_crispr.py
@@ -35,6 +35,8 @@ def test_crispr():
         a.rc(),
     ]
 
+    assert target.cut(cas9("GTTACTTTACCCGACGTCCC")) == target.cut(cas9("GTTACTTTACCCGACGTCCC".lower()))
+
 
 if __name__ == "__main__":
     pytest.main([__file__, "-vv", "-s"])