diff --git a/Tests/test_file_libtiff.py b/Tests/test_file_libtiff.py
index 69499ff6ba8..a7394f1bf5c 100644
--- a/Tests/test_file_libtiff.py
+++ b/Tests/test_file_libtiff.py
@@ -1035,7 +1035,10 @@ def test_orientation(self):
         with Image.open("Tests/images/g4_orientation_1.tif") as base_im:
             for i in range(2, 9):
                 with Image.open("Tests/images/g4_orientation_" + str(i) + ".tif") as im:
+                    assert 274 in im.tag_v2
+
                     im.load()
+                    assert 274 not in im.tag_v2
 
                     assert_image_similar(base_im, im, 0.7)
 
diff --git a/src/PIL/ImageOps.py b/src/PIL/ImageOps.py
index 1231ad6ebda..ce2b1bd9b4d 100644
--- a/src/PIL/ImageOps.py
+++ b/src/PIL/ImageOps.py
@@ -611,6 +611,10 @@ def exif_transpose(image, *, in_place=False):
         exif = exif_image.getexif()
         if ExifTags.Base.Orientation in exif:
             del exif[ExifTags.Base.Orientation]
+            if in_place and ExifTags.Base.Orientation in getattr(
+                exif_image, "tag_v2", {}
+            ):
+                del exif_image.tag_v2[ExifTags.Base.Orientation]
             if "exif" in exif_image.info:
                 exif_image.info["exif"] = exif.tobytes()
             elif "Raw profile type exif" in exif_image.info: