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Major change to TRIM with new behavior. * Added -p flag to turn on padding of paired-end reads with N’s, such that reads are same length. This was the default for paired-end reads. THIS IS NO LONGER ON BY DEFAULT as this has potential adverse affects with the STAR aligner. * Set -m default to 0 and regain original functionality with change to PIPELINE.
Major changes to TRIM. * Added quality score thresholding. * changed behavior of -c: users can run TRIM without a contaminants file. Pre-existing behavior encoded in PIPELINE. * changed behavior of -kN (replaced with -rN): now the default is to not remove N’s from end. Use -rN to remove N’s which is consistent with trimReads.py. The -rN flag has been added to PIPELINE. * changed behavior of -rAT: now default is not removing poly-A/T. Pre-existing behavior (trim 26 bases) now coded in PIPELINE.
minor tweak.
moved repo description to own page, documented globals Documented the global variables that are hardcoded in ngs.sh Moved the repository description from Home to DataRepo.
tweak
updated todo. fixed TRIM docs.
tweaks
expanded TRIM
added TRIM