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I used Seurat for my image-based spatial data from nanostring. It works great. Instead of performing unsupervised analysis, I followed the instructions to map the profiles to Azimuth Human Pancreas reference as "pancreas.object <- RunAzimuth( pancreas.object, reference = "pancreasref" )" Thanks for @AustinHartman's help.
Now I would like to do the same thing with my own single-cell RNA-seq data as the reference. To my understanding, I can use "AzimuthReference" to generate the reference to do so. But I did not find the instruction, protocol, or example of how. I am very new to Seurat, could you please give me some guidance on it? Thanks a lot!
The text was updated successfully, but these errors were encountered:
I used Seurat for my image-based spatial data from nanostring. It works great. Instead of performing unsupervised analysis, I followed the instructions to map the profiles to Azimuth Human Pancreas reference as "pancreas.object <- RunAzimuth( pancreas.object, reference = "pancreasref" )" Thanks for @AustinHartman's help.
Now I would like to do the same thing with my own single-cell RNA-seq data as the reference. To my understanding, I can use "AzimuthReference" to generate the reference to do so. But I did not find the instruction, protocol, or example of how. I am very new to Seurat, could you please give me some guidance on it? Thanks a lot!
The text was updated successfully, but these errors were encountered: