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alignment_metrics.md

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QC pipeline metrics

Column Description
cell_id label of the cell
index_sequence index sequence of the adaptor sequence
column column of the cell on the nanowell chip
img_col column of the cell from the perspective of the microscope
index_i5 id of the i5 index adapter sequence
sample_type type of the sample
primer_i7 id of the i5 index primer sequence
experimental_condition experimental treatment of the cell, includes controls
index_i7 id of the i7 index adapter sequence
cell_call living/dead classification of the cell based on staining usually, C1 == living, C2 == dead
sample_id name of the sample
primer_i5 id of the i5 index primer sequence
row row of the cell on the nanowell chip
estimated_library_size scaled total number of mapped reads
total_mapped_reads total number of mapped reads
nohit number of reads with no organism match
salmon_multihit number of reads that were classified as salmon and something else
total_duplicate_reads number of duplicate reads
percent_duplicate_reads percentage of duplicate reads
total_properly_paired number of properly paired reads
mean_insert_size mean insert size between paired reads
coverage_breadth percentage of genome covered by some read
grch37 number of reads that were classified as human
unpaired_duplicate_reads number of unpaired duplicated reads
unpaired_mapped_reads number of unpaired mapped reads
unmapped_reads number of unmapped reads
coverage_depth average reads per nucleotide position in the genome
median_insert_size median insert size between paired reads
salmon number of reads that were classified as salmon
grch37_multihit number of reads that were classified as human and something else
mm10 number of reads that were classified as mouse
total_reads total number of reads, regardless of mapping status
standard_deviation_insert_size standard deviation of the insert size between paired reads
paired_mapped_reads number of mapped reads that were properly paired
mm10_multihit number of reads classified as mouse and something else
paired_duplicate_reads number of paired reads that were also marked as duplicate