diff --git a/README.md b/README.md index 50a5396..9de9cf1 100644 --- a/README.md +++ b/README.md @@ -10,7 +10,8 @@ TODO: Add a brief intro ## TODO: -* Clean up all un-used code +* Clean up all notebooks +* Remove all unused code * Provide basic comments in all scripts * In each README section, write a short para or few sentences about the analysis. @@ -63,7 +64,7 @@ Additional plotting ## Differential expression analysis (Figs. 2 and 4) -NoteL:Need to find from Emily's codebase: +Note:Need to find from Emily's codebase: * Human ST marker genes * Human ST condition @@ -71,9 +72,11 @@ NoteL:Need to find from Emily's codebase: All snRNA-seq DEG scripts are there +Make a separate script for the Pseudobulk comparisons that we used for the peer review doc? + ### snRNA-seq differential expression -TODO: Split the Sex analysis by snRNA vs ST ? +TODO: Split the Sex analysis script by snRNA vs ST ? * [Marker genes](snRNA/differential_expression/markers/) * [Disease vs Control DEG scripts](snRNA/differential_expression/condition/) @@ -117,7 +120,21 @@ Mouse dataset ## Predicting spatial coordinates for snRNA-seq data (Extended Data Fig. 5) +TODO: cleanup the .Rmd scripts + +* [Functions to run CellTrek in parallel for each snRNA-ST pair](snRNA/predict_spatial_coords/celltrek_parallel.R) +* [Running CellTrek in parallel for each snRNA-ST pair](snRNA/predict_spatial_coords/cellrtrek_parallel_run.sub) +* [Downstream plotting script pt. 1](snRNA/predict_spatial_coords/cellrtrek_downsrtream_plotting.Rmd) +* [Downstream plotting script pt. 2](snRNA/predict_spatial_coords/spatial_mapping_celltrek.Rmd) + + ## Cell-cell communication (CCC) network analysis (Fig. 6) +* [Cell-cell-communication network analysis with CellChat](snRNA/cell_cell_communication/spatial_cellchat.Rmd) +* [Cell-cell-communication network analysis with LIANA and comparing with CellChat](snRNA/cell_cell_communication/LIANA_ccc.Rmd) + ## Amyloid-associated gene expression signatures (Fig. 7) +* [Integration of amyloid imaging with ST datasets](spatial/amyloid/splitseq_human_amyloid.Rmd) +* [Human amyloid analysis](spatial/amyloid/voyager_gene_signatures_human.Rmd) +* [Mouse amyloid analysis](spatial/amyloid/voyager_gene_signatures_5x.Rmd) \ No newline at end of file