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Please add output for % reads aligned for Anippy_Variants
📚 Context
Currently, Snipy_Variants provides an output column of # reads aligned, but this isn't put in context of the total number of reads in the sample. It would be very helpful to have a % calculated to be able to see if there are any major differences in the samples vs the ref, or any contamination indicated by low % reads aligned.
📈 Desired Behavior
Add extra output column with %reads aligned
ℹ️ Additional Information
The text was updated successfully, but these errors were encountered:
Looking at #592, I think we may have the numbers already available and only need to be calculated. For example, If I look at this new combined QC output:
samplename
reads_aligned_to_reference
variants_total
percent_ref_coverage
#rname
startpos
endpos
numreads
covbases
coverage
meandepth
meanbaseq
meanmapq
#rname
startpos
endpos
numreads
covbases
coverage
meandepth
meanbaseq
meanmapq
ERR10913488
3504551
84125
84.5073
AE003852
1
2961182
2376498
2615376
88.322
114.7
34.2
58.6
AE003853
1
1072319
654185
830592
77.4575
87.0957
34.2
59
we can see that 3504551 were aligned to reference, whereas 2376498 and 654185 (total: 3030683) were mapped to chromosomes 1 and 2, and 86.5% of the reads.
🆒
📌 Explain the Request
Please add output for % reads aligned for Anippy_Variants
📚 Context
Currently, Snipy_Variants provides an output column of # reads aligned, but this isn't put in context of the total number of reads in the sample. It would be very helpful to have a % calculated to be able to see if there are any major differences in the samples vs the ref, or any contamination indicated by low % reads aligned.
📈 Desired Behavior
Add extra output column with %reads aligned
ℹ️ Additional Information
The text was updated successfully, but these errors were encountered: