diff --git a/.buildkite/scripts/steps/checks/quick_checks.txt b/.buildkite/scripts/steps/checks/quick_checks.txt
index 9bd9224673905..93c192d683dfc 100644
--- a/.buildkite/scripts/steps/checks/quick_checks.txt
+++ b/.buildkite/scripts/steps/checks/quick_checks.txt
@@ -17,3 +17,4 @@
 .buildkite/scripts/steps/checks/prettier_topology.sh
 .buildkite/scripts/steps/checks/renovate.sh
 .buildkite/scripts/steps/checks/native_modules.sh
+.buildkite/scripts/steps/checks/styled_components_mapping.sh
diff --git a/.buildkite/scripts/steps/checks/styled_components_mapping.sh b/.buildkite/scripts/steps/checks/styled_components_mapping.sh
new file mode 100644
index 0000000000000..2815bf41180b5
--- /dev/null
+++ b/.buildkite/scripts/steps/checks/styled_components_mapping.sh
@@ -0,0 +1,11 @@
+#!/usr/bin/env bash
+
+set -euo pipefail
+
+source .buildkite/scripts/common/util.sh
+
+echo --- Check styled-components mapping
+cmd="node scripts/styled_components_mapping"
+
+eval "$cmd"
+check_for_changed_files "$cmd" true
diff --git a/.github/CODEOWNERS b/.github/CODEOWNERS
index 3074d95d25077..0362a5210fd45 100644
--- a/.github/CODEOWNERS
+++ b/.github/CODEOWNERS
@@ -1041,6 +1041,7 @@ src/platform/packages/shared/kbn-xstate-utils @elastic/obs-ux-logs-team
 packages/kbn-yarn-lock-validator @elastic/kibana-operations
 src/platform/packages/shared/kbn-zod @elastic/kibana-core
 src/platform/packages/shared/kbn-zod-helpers @elastic/security-detection-rule-management
+packages/kbn-styled-components-mapping-cli @elastic/kibana-operations @elastic/eui-team
 ####
 ## Everything below this line overrides the default assignments for each package.
 ## Items lower in the file have higher precedence:
diff --git a/package.json b/package.json
index 4a3a0527a4800..eb52a1fb53d90 100644
--- a/package.json
+++ b/package.json
@@ -1504,6 +1504,7 @@
     "@kbn/spec-to-console": "link:packages/kbn-spec-to-console",
     "@kbn/stdio-dev-helpers": "link:packages/kbn-stdio-dev-helpers",
     "@kbn/storybook": "link:packages/kbn-storybook",
+    "@kbn/styled-components-mapping-cli": "link:packages/kbn-styled-components-mapping-cli",
     "@kbn/synthetics-e2e": "link:x-pack/solutions/observability/plugins/synthetics/e2e",
     "@kbn/synthetics-private-location": "link:x-pack/packages/kbn-synthetics-private-location",
     "@kbn/telemetry-tools": "link:packages/kbn-telemetry-tools",
diff --git a/packages/kbn-styled-components-mapping-cli/README.md b/packages/kbn-styled-components-mapping-cli/README.md
new file mode 100644
index 0000000000000..42ed1cde6149e
--- /dev/null
+++ b/packages/kbn-styled-components-mapping-cli/README.md
@@ -0,0 +1,10 @@
+# @kbn/styled-components-mapping-cli
+
+A helper tool that looks up components using `styled-components` and generates
+a mapping file for Babel to help with proper stylesheet loading.
+
+## Usage
+
+```shell
+node scripts/styled_components_mapping
+```
diff --git a/packages/kbn-styled-components-mapping-cli/jest.config.js b/packages/kbn-styled-components-mapping-cli/jest.config.js
new file mode 100644
index 0000000000000..f4b92ce3bcd3f
--- /dev/null
+++ b/packages/kbn-styled-components-mapping-cli/jest.config.js
@@ -0,0 +1,14 @@
+/*
+ * Copyright Elasticsearch B.V. and/or licensed to Elasticsearch B.V. under one
+ * or more contributor license agreements. Licensed under the "Elastic License
+ * 2.0", the "GNU Affero General Public License v3.0 only", and the "Server Side
+ * Public License v 1"; you may not use this file except in compliance with, at
+ * your election, the "Elastic License 2.0", the "GNU Affero General Public
+ * License v3.0 only", or the "Server Side Public License, v 1".
+ */
+
+module.exports = {
+  preset: '@kbn/test/jest_node',
+  rootDir: '../..',
+  roots: ['<rootDir>/packages/kbn-styled-components-mapping-cli'],
+};
diff --git a/packages/kbn-styled-components-mapping-cli/kibana.jsonc b/packages/kbn-styled-components-mapping-cli/kibana.jsonc
new file mode 100644
index 0000000000000..282032b252dad
--- /dev/null
+++ b/packages/kbn-styled-components-mapping-cli/kibana.jsonc
@@ -0,0 +1,6 @@
+{
+  "type": "shared-common",
+  "id": "@kbn/styled-components-mapping-cli",
+  "owner": ["@elastic/kibana-operations", "@elastic/eui-team"],
+  "devOnly": true
+}
diff --git a/packages/kbn-styled-components-mapping-cli/package.json b/packages/kbn-styled-components-mapping-cli/package.json
new file mode 100644
index 0000000000000..43f8f2d625565
--- /dev/null
+++ b/packages/kbn-styled-components-mapping-cli/package.json
@@ -0,0 +1,7 @@
+{
+  "name": "@kbn/styled-components-mapping-cli",
+  "private": true,
+  "version": "1.0.0",
+  "license": "Elastic License 2.0 OR AGPL-3.0-only OR SSPL-1.0",
+  "main": "./src/run_styled_components_mapping_cli.ts"
+}
\ No newline at end of file
diff --git a/packages/kbn-styled-components-mapping-cli/src/find_files.ts b/packages/kbn-styled-components-mapping-cli/src/find_files.ts
new file mode 100644
index 0000000000000..a984c6a84e04e
--- /dev/null
+++ b/packages/kbn-styled-components-mapping-cli/src/find_files.ts
@@ -0,0 +1,79 @@
+/*
+ * Copyright Elasticsearch B.V. and/or licensed to Elasticsearch B.V. under one
+ * or more contributor license agreements. Licensed under the "Elastic License
+ * 2.0", the "GNU Affero General Public License v3.0 only", and the "Server Side
+ * Public License v 1"; you may not use this file except in compliance with, at
+ * your election, the "Elastic License 2.0", the "GNU Affero General Public
+ * License v3.0 only", or the "Server Side Public License, v 1".
+ */
+
+import * as fs from 'node:fs/promises';
+import * as path from 'node:path';
+
+const SOURCE_DIRS = ['x-pack', 'src', 'packages'];
+const SOURCE_FILE_REGEX = /(^.?|\.[^d]|[^.]d|[^.][^d])\.tsx?$/;
+const STYLED_COMPONENTS_IMPORT_REGEX =
+  /import\s+(?:{[^{}]+}|.*?)\s*(?:from)?\s*['"](styled-components)['"]/;
+const EMOTION_STYLED_IMPORT_REGEX =
+  /import\s+(?:{[^{}]+}|.*?)\s*(?:from)?\s*['"](@emotion\/styled)['"]/;
+
+export interface Meta {
+  path: string;
+  usesStyledComponents: boolean;
+  usesOnlyStyledComponents?: boolean;
+  files?: Meta[];
+}
+
+const walkDirectory = async (dirPath: string): Promise<Meta> => {
+  const files: Meta[] = [];
+  let usesStyledComponents = false;
+  let usesOnlyStyledComponents = true;
+
+  for (const file of await fs.readdir(dirPath, { withFileTypes: true })) {
+    const fullPath = path.join(file.path, file.name);
+
+    if (file.isDirectory()) {
+      const meta = await walkDirectory(fullPath);
+      if (meta.usesStyledComponents) {
+        usesStyledComponents = true;
+      }
+      if (usesOnlyStyledComponents && !meta.usesOnlyStyledComponents) {
+        usesOnlyStyledComponents = false;
+      }
+      files.push(meta);
+      continue;
+    }
+
+    if (!SOURCE_FILE_REGEX.test(file.name) || !file.isFile()) {
+      continue;
+    }
+
+    const meta: Meta = {
+      path: fullPath,
+      usesStyledComponents: false,
+    };
+
+    const contents = await fs.readFile(fullPath, 'utf8');
+    const usesEmotionStyled = EMOTION_STYLED_IMPORT_REGEX.test(contents);
+    meta.usesStyledComponents = STYLED_COMPONENTS_IMPORT_REGEX.test(contents);
+
+    if (usesEmotionStyled) {
+      usesOnlyStyledComponents = false;
+    }
+
+    if (usesEmotionStyled || meta.usesStyledComponents) {
+      files.push(meta);
+    }
+  }
+
+  return {
+    path: dirPath,
+    files,
+    usesStyledComponents,
+    usesOnlyStyledComponents: usesStyledComponents && usesOnlyStyledComponents,
+  };
+};
+
+export const findFiles = async (rootDirPath: string) => {
+  return Promise.all(SOURCE_DIRS.map((dir) => walkDirectory(path.join(rootDirPath, dir))));
+};
diff --git a/packages/kbn-styled-components-mapping-cli/src/generate_regex_array.ts b/packages/kbn-styled-components-mapping-cli/src/generate_regex_array.ts
new file mode 100644
index 0000000000000..529749edc4371
--- /dev/null
+++ b/packages/kbn-styled-components-mapping-cli/src/generate_regex_array.ts
@@ -0,0 +1,35 @@
+/*
+ * Copyright Elasticsearch B.V. and/or licensed to Elasticsearch B.V. under one
+ * or more contributor license agreements. Licensed under the "Elastic License
+ * 2.0", the "GNU Affero General Public License v3.0 only", and the "Server Side
+ * Public License v 1"; you may not use this file except in compliance with, at
+ * your election, the "Elastic License 2.0", the "GNU Affero General Public
+ * License v3.0 only", or the "Server Side Public License, v 1".
+ */
+
+import * as path from 'node:path';
+import type { Meta } from './find_files';
+
+export const pathToRegexString = (modulePath: string): string => {
+  modulePath = modulePath.replace(/[\\\/]/g, '[\\/\\\\]');
+
+  return `/${modulePath}/`;
+};
+
+export const generateRegexStringArray = (files: Meta[], rootDirPath: string): string[] => {
+  const array: string[] = [];
+
+  for (const meta of files) {
+    if (meta.files) {
+      if (meta.usesOnlyStyledComponents) {
+        array.push(pathToRegexString(path.relative(rootDirPath, meta.path)));
+      } else {
+        array.push(...generateRegexStringArray(meta.files, rootDirPath));
+      }
+    } else if (meta.usesStyledComponents) {
+      array.push(pathToRegexString(path.relative(rootDirPath, meta.path)));
+    }
+  }
+
+  return array;
+};
diff --git a/packages/kbn-styled-components-mapping-cli/src/run_styled_components_mapping_cli.ts b/packages/kbn-styled-components-mapping-cli/src/run_styled_components_mapping_cli.ts
new file mode 100644
index 0000000000000..cfad8da6446fa
--- /dev/null
+++ b/packages/kbn-styled-components-mapping-cli/src/run_styled_components_mapping_cli.ts
@@ -0,0 +1,40 @@
+/*
+ * Copyright Elasticsearch B.V. and/or licensed to Elasticsearch B.V. under one
+ * or more contributor license agreements. Licensed under the "Elastic License
+ * 2.0", the "GNU Affero General Public License v3.0 only", and the "Server Side
+ * Public License v 1"; you may not use this file except in compliance with, at
+ * your election, the "Elastic License 2.0", the "GNU Affero General Public
+ * License v3.0 only", or the "Server Side Public License, v 1".
+ */
+
+import * as path from 'node:path';
+import { run } from '@kbn/dev-cli-runner';
+import { createFailError } from '@kbn/dev-cli-errors';
+import { REPO_ROOT } from '@kbn/repo-info';
+import { findFiles } from './find_files';
+import { generateRegexStringArray } from './generate_regex_array';
+import { updateFile } from './update_file';
+
+const mappingFilePath = 'packages/kbn-babel-preset/styled_components_files.js';
+const mappingFileAbsolutePath = path.join(REPO_ROOT, mappingFilePath);
+
+run(
+  async ({ log }) => {
+    log.info(`Looking for source files importing 'styled-components'...`);
+
+    const files = await findFiles(REPO_ROOT);
+    const regexStringArray = generateRegexStringArray(files, REPO_ROOT);
+
+    try {
+      await updateFile(mappingFileAbsolutePath, regexStringArray);
+    } catch (err) {
+      createFailError(err);
+    }
+
+    log.info(`Generated ${mappingFilePath} with ${regexStringArray.length} regex expressions`);
+  },
+  {
+    usage: `node scripts/styled_components_mapping`,
+    description: 'Update styled-components babel mapping when converting styles to Emotion',
+  }
+);
diff --git a/packages/kbn-styled-components-mapping-cli/src/update_file.ts b/packages/kbn-styled-components-mapping-cli/src/update_file.ts
new file mode 100644
index 0000000000000..5e995e43741d2
--- /dev/null
+++ b/packages/kbn-styled-components-mapping-cli/src/update_file.ts
@@ -0,0 +1,48 @@
+/*
+ * Copyright Elasticsearch B.V. and/or licensed to Elasticsearch B.V. under one
+ * or more contributor license agreements. Licensed under the "Elastic License
+ * 2.0", the "GNU Affero General Public License v3.0 only", and the "Server Side
+ * Public License v 1"; you may not use this file except in compliance with, at
+ * your election, the "Elastic License 2.0", the "GNU Affero General Public
+ * License v3.0 only", or the "Server Side Public License, v 1".
+ */
+
+import * as fs from 'node:fs/promises';
+
+const babelFilePrefix = `/*
+ * Copyright Elasticsearch B.V. and/or licensed to Elasticsearch B.V. under one
+ * or more contributor license agreements. Licensed under the "Elastic License
+ * 2.0", the "GNU Affero General Public License v3.0 only", and the "Server Side
+ * Public License v 1"; you may not use this file except in compliance with, at
+ * your election, the "Elastic License 2.0", the "GNU Affero General Public
+ * License v3.0 only", or the "Server Side Public License, v 1".
+ */
+
+module.exports = {
+  /**
+   * Synchronized list of all source files that use styled-components.
+   * Please keep this list up-to-date when converting component styles
+   * from styled-components to Emotion.
+   *
+   * Babel's MatchPattern can be a regex or a string which follows standard
+   * Node.js path logic as described here:
+   * https://babeljs.io/docs/options#matchpattern
+   *
+   * Used by \`kbn-babel-preset\` and \`kbn-eslint-config\`.
+   */
+  USES_STYLED_COMPONENTS: [
+    /packages[\\/\\\\]kbn-ui-shared-deps-npm[\\/\\\\]/,
+    /packages[\\/\\\\]kbn-ui-shared-deps-src[\\/\\\\]/,
+`;
+
+const babelFileSuffix = `  ],
+};
+`;
+
+export const updateFile = async (filePath: string, regexStringArray: string[]) => {
+  const contents = `${babelFilePrefix}\n    ${regexStringArray.join(
+    ',\n    '
+  )},\n${babelFileSuffix}`;
+
+  return fs.writeFile(filePath, contents);
+};
diff --git a/packages/kbn-styled-components-mapping-cli/tsconfig.json b/packages/kbn-styled-components-mapping-cli/tsconfig.json
new file mode 100644
index 0000000000000..1e44316610f69
--- /dev/null
+++ b/packages/kbn-styled-components-mapping-cli/tsconfig.json
@@ -0,0 +1,21 @@
+{
+  "extends": "../../tsconfig.base.json",
+  "compilerOptions": {
+    "outDir": "target/types",
+    "types": [
+      "jest",
+      "node"
+    ]
+  },
+  "include": [
+    "**/*.ts",
+  ],
+  "exclude": [
+    "target/**/*"
+  ],
+  "kbn_references": [
+    "@kbn/dev-cli-runner",
+    "@kbn/dev-cli-errors",
+    "@kbn/repo-info",
+  ]
+}
diff --git a/scripts/styled_components_mapping.js b/scripts/styled_components_mapping.js
new file mode 100644
index 0000000000000..770155b7882f7
--- /dev/null
+++ b/scripts/styled_components_mapping.js
@@ -0,0 +1,11 @@
+/*
+ * Copyright Elasticsearch B.V. and/or licensed to Elasticsearch B.V. under one
+ * or more contributor license agreements. Licensed under the "Elastic License
+ * 2.0", the "GNU Affero General Public License v3.0 only", and the "Server Side
+ * Public License v 1"; you may not use this file except in compliance with, at
+ * your election, the "Elastic License 2.0", the "GNU Affero General Public
+ * License v3.0 only", or the "Server Side Public License, v 1".
+ */
+
+require('../src/setup_node_env');
+require('@kbn/styled-components-mapping-cli');
diff --git a/tsconfig.base.json b/tsconfig.base.json
index c3375458959b7..a3c7efe962b6b 100644
--- a/tsconfig.base.json
+++ b/tsconfig.base.json
@@ -1878,6 +1878,8 @@
       "@kbn/streams-plugin/*": ["x-pack/solutions/observability/plugins/streams/*"],
       "@kbn/streams-schema": ["x-pack/packages/kbn-streams-schema"],
       "@kbn/streams-schema/*": ["x-pack/packages/kbn-streams-schema/*"],
+      "@kbn/styled-components-mapping-cli": ["packages/kbn-styled-components-mapping-cli"],
+      "@kbn/styled-components-mapping-cli/*": ["packages/kbn-styled-components-mapping-cli/*"],
       "@kbn/synthetics-e2e": ["x-pack/solutions/observability/plugins/synthetics/e2e"],
       "@kbn/synthetics-e2e/*": ["x-pack/solutions/observability/plugins/synthetics/e2e/*"],
       "@kbn/synthetics-plugin": ["x-pack/solutions/observability/plugins/synthetics"],
diff --git a/yarn.lock b/yarn.lock
index 8e2144b587caf..5c85f57ae1f12 100644
--- a/yarn.lock
+++ b/yarn.lock
@@ -7608,6 +7608,10 @@
   version "0.0.0"
   uid ""
 
+"@kbn/styled-components-mapping-cli@link:packages/kbn-styled-components-mapping-cli":
+  version "0.0.0"
+  uid ""
+
 "@kbn/synthetics-e2e@link:x-pack/solutions/observability/plugins/synthetics/e2e":
   version "0.0.0"
   uid ""