diff --git a/PangolinColab.ipynb b/PangolinColab.ipynb
index 833a47a..063b717 100644
--- a/PangolinColab.ipynb
+++ b/PangolinColab.ipynb
@@ -50,7 +50,7 @@
         "id": "NpEQ3rtI9BI6"
       },
       "source": [
-        "!pip install pyvcf gffutils biopython pandas pyfastx\n",
+        "!pip install pyvcf gffutils pandas pyfastx\n",
         "!git clone https://github.com/tkzeng/Pangolin.git\n",
         "%cd Pangolin\n",
         "!pip install .\n",
@@ -139,4 +139,4 @@
       "outputs": []
     }
   ]
-}
\ No newline at end of file
+}
diff --git a/README.md b/README.md
index 912ddb9..f3ae4b8 100755
--- a/README.md
+++ b/README.md
@@ -13,7 +13,7 @@ See below for information on usage and local installation.
 * Install other dependencies:
   ```
   conda install -c conda-forge pyvcf
-  pip install gffutils biopython pandas pyfastx
+  pip install gffutils pandas pyfastx
   ```
 * Install Pangolin:
   ```