diff --git a/tests/lib/devicetree/memory_region_flags/CMakeLists.txt b/tests/lib/devicetree/memory_region_flags/CMakeLists.txt
new file mode 100644
index 00000000000000..178264b34a13d8
--- /dev/null
+++ b/tests/lib/devicetree/memory_region_flags/CMakeLists.txt
@@ -0,0 +1,9 @@
+# SPDX-License-Identifier: Apache-2.0
+
+cmake_minimum_required(VERSION 3.20.0)
+
+find_package(Zephyr REQUIRED HINTS $ENV{ZEPHYR_BASE})
+project(memory_region_flags)
+
+FILE(GLOB app_sources src/*.c)
+target_sources(app PRIVATE ${app_sources})
diff --git a/tests/lib/devicetree/memory_region_flags/app.overlay b/tests/lib/devicetree/memory_region_flags/app.overlay
new file mode 100644
index 00000000000000..481ac6ce8aaf4d
--- /dev/null
+++ b/tests/lib/devicetree/memory_region_flags/app.overlay
@@ -0,0 +1,37 @@
+/*
+ * Copyright (c) 2024, TOKITA Hiroshi <tokita.hiroshi@gmail.com>
+ */
+
+/ {
+	test {
+		#address-cells = < 0x1 >;
+		#size-cells = < 0x1 >;
+
+		test_region_r: sram@20010000 {
+			compatible = "zephyr,memory-region";
+			reg = < 0x20010000 0x100 >;
+			zephyr,memory-region = "TEST_REGION_R";
+			zephyr,memory-region-flags = "r";
+		};
+
+		test_region_nrwxail: sram@20010100 {
+			compatible = "zephyr,memory-region";
+			reg = < 0x20010100 0x100 >;
+			zephyr,memory-region = "TEST_REGION_NRWXAIL";
+			zephyr,memory-region-flags = "!rwxail";
+		};
+
+		test_region_no_flags: sram@20010200 {
+			compatible = "zephyr,memory-region";
+			reg = < 0x20010200 0x100 >;
+			zephyr,memory-region = "TEST_REGION_NO_FLAGS";
+		};
+
+		test_region_none: sram@20010300 {
+			compatible = "zephyr,memory-region";
+			reg = < 0x20010300 0x100 >;
+			zephyr,memory-region = "TEST_REGION_NONE";
+			zephyr,memory-region-flags = "";
+		};
+	};
+};
diff --git a/tests/lib/devicetree/memory_region_flags/prj.conf b/tests/lib/devicetree/memory_region_flags/prj.conf
new file mode 100644
index 00000000000000..1bb8bf6d7fd4c8
--- /dev/null
+++ b/tests/lib/devicetree/memory_region_flags/prj.conf
@@ -0,0 +1 @@
+# empty
diff --git a/tests/lib/devicetree/memory_region_flags/pytest/test_memory_region_flags.py b/tests/lib/devicetree/memory_region_flags/pytest/test_memory_region_flags.py
new file mode 100644
index 00000000000000..d25a03a49aa736
--- /dev/null
+++ b/tests/lib/devicetree/memory_region_flags/pytest/test_memory_region_flags.py
@@ -0,0 +1,73 @@
+# Copyright (c) 2024, TOKITA Hiroshi <tokita.hiroshi@gmail.com>
+# SPDX-License-Identifier: Apache-2.0
+
+import os
+import sys
+import logging
+import re
+from twister_harness import DeviceAdapter
+
+ZEPHYR_BASE = os.getenv("ZEPHYR_BASE")
+sys.path.insert(
+    0, os.path.join(ZEPHYR_BASE, "scripts", "dts", "python-devicetree", "src")
+)
+from devicetree import edtlib
+
+logger = logging.getLogger(__name__)
+
+
+def smart_int(numstr):
+    if numstr.startswith("0x"):
+        return int(numstr[2:], 16)
+    else:
+        return int(numstr)
+
+
+def verify_memory_region_flags(build_dir, label, expect):
+    logger.info(label)
+
+    linkercmd = os.path.join(build_dir, "zephyr", "linker.cmd")
+    logger.info(linkercmd)
+
+    edt = edtlib.EDT(
+        os.path.join(build_dir, "zephyr", "zephyr.dts"),
+        [os.path.join(ZEPHYR_BASE, "dts", "bindings")],
+    )
+
+    node = edt.label2node[label]
+    logger.info(node)
+
+    region = node.props["zephyr,memory-region"].val
+    logger.info(region)
+
+    origin = node.props["reg"].val[0]
+    length = node.props["reg"].val[1]
+    pattern = (
+        region + r"\s*" + expect +
+        r"\s*:\s*ORIGIN\s*=\s*\(?([0-9A-Fa-fx]*)\)?,\s*LENGTH\s*=\s*\(?([0-9A-Fa-fx]*)\)?"
+    )
+
+    logger.info(pattern)
+
+    found = False
+
+    with open(linkercmd) as f:
+        for line in f:
+            m = re.search(pattern, line)
+            if m and smart_int(m[1]) == origin and smart_int(m[2]) == length:
+                found = True
+
+    assert found
+
+
+def test_region_r(dut: DeviceAdapter):
+    verify_memory_region_flags(dut.device_config.build_dir, "test_region_r", r"\(\s*r\s*\)")
+
+def test_region_nrwxail(dut: DeviceAdapter):
+    verify_memory_region_flags(dut.device_config.build_dir, "test_region_nrwxail", r"\(\s*!rwxail\s*\)")
+
+def test_region_no_flags(dut: DeviceAdapter):
+    verify_memory_region_flags(dut.device_config.build_dir, "test_region_no_flags", r"\(\s*rw\s*\)")
+
+def test_region_none(dut: DeviceAdapter):
+    verify_memory_region_flags(dut.device_config.build_dir, "test_region_none", r"")
diff --git a/tests/lib/devicetree/memory_region_flags/src/main.c b/tests/lib/devicetree/memory_region_flags/src/main.c
new file mode 100644
index 00000000000000..af84bb23479274
--- /dev/null
+++ b/tests/lib/devicetree/memory_region_flags/src/main.c
@@ -0,0 +1,10 @@
+/*
+ * Copyright (c) 2024, TOKITA Hiroshi <tokita.hiroshi@gmail.com>
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+int main(void)
+{
+	return 0;
+}
diff --git a/tests/lib/devicetree/memory_region_flags/testcase.yaml b/tests/lib/devicetree/memory_region_flags/testcase.yaml
new file mode 100644
index 00000000000000..893e118976d2d5
--- /dev/null
+++ b/tests/lib/devicetree/memory_region_flags/testcase.yaml
@@ -0,0 +1,17 @@
+tests:
+
+  libraries.devicetree.memory_region_flags:
+    harness: pytest
+    platform_allow: qemu_cortex_m3
+    tags:
+      - devicetree
+      - pytest
+
+  libraries.devicetree.memory_region_flags.linker_generator:
+    harness: pytest
+    platform_allow: qemu_cortex_m3
+    tags:
+      - devicetree
+      - pytest
+    extra_configs:
+      - CONFIG_CMAKE_LINKER_GENERATOR=y