diff --git a/tests/drivers/i2c/i2c_target_api/boards/frdm_mcxa156.overlay b/tests/drivers/i2c/i2c_target_api/boards/frdm_mcxa156.overlay
new file mode 100644
index 000000000000000..d82f84ab3de3e9a
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/frdm_mcxa156.overlay
@@ -0,0 +1,26 @@
+/*
+ * Copyright 2024 NXP
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/* To test this sample, connect
+ * LPI2C0 SCL(J2-20)  -->  LPI2C3 SCL(J5-5)
+ * LPI2C0 SDA(J2-18)  -->  LPI2C3 SDA(J5-6)
+ */
+
+&lpi2c0 {
+	eeprom0: eeprom@54 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x54>;
+		size = <256>;
+	};
+};
+
+&lpi2c3 {
+	eeprom1: eeprom@56 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x56>;
+		size = <256>;
+	};
+};
diff --git a/tests/drivers/i2c/i2c_target_api/testcase.yaml b/tests/drivers/i2c/i2c_target_api/testcase.yaml
index bf931f8a08d1d20..d57b121582acbbb 100644
--- a/tests/drivers/i2c/i2c_target_api/testcase.yaml
+++ b/tests/drivers/i2c/i2c_target_api/testcase.yaml
@@ -45,6 +45,7 @@ tests:
       - mimxrt1060_evk
       - frdm_ke17z512
       - frdm_mcxn236
+      - frdm_mcxa156
       - max32655evkit/max32655/m4
       - max32662evkit
       - max32666evkit/max32666/cpu0