My name is Ana, and I currently live near Portland, OR.

I have extensive experience in various areas of immunology research, including kidney/liver transplant and immuno-oncology. As a bench scientist, I have developed fundamental molecular skills related to biobanking, cryopreservation, logistics, clinical trials, and flow cytometry analysis.

In 2018, I began my training as a bioinformatician, driven by my curiosity about the R language and single-cell transcriptomics. Since then, I have worked with different versions of this technology, including multimodal 10x genomics analysis using scRNAseq, CITE-seq, HTO (hashtag), TCR-seq, some scATAC-seq, and currently, snRNAseq for non-immune cells. I have utilized various platforms for large-scale computing, such as HPCs, AWS, and GCP. Most of the analysis tools my team uses are centered around Bioconductor tools and the R language for creating reports and reproducible analyses. We also spend part of our time testing larger Python libraries to aggregate internal datasets using the scverse framework and platforms like Databricks and TileDB (gotta love our data engineers! 🤓).

For the past year and a half, I have primarily focused on testing downstream analysis packages for a project involving snRNAseq in liver samples from patients diagnosed with Metabolic-associated Steatohepatitis (MASH). We have evaluated tools for cell-cell communication, subtype annotation, and pseudobulk differential expression analysis. Our projects are heavily centered around the biological question 🧬 and the commercialization of potential drug targets.

My current project involves a large collaborative effort between multiple centers to analyze snRNAseq data from a large cohort of patients with MASH. Consequently, I am dedicated to developing scalable analysis pipelines that are well-documented and facilitate report generation, all while managing an ongoing wave of batches for analysis throughout the year.

I love what I do and I would love to get in touch. 🤝

As for personal projects and what I can share with you on github...

• 💻 I’m currently working on updating my github profile, starting a blog, and showcasing my skills
• 📚 I am reading about Immuno-tolerance and allergies, and turning personal interests into a framework to write about in my blog
• 💬 Ask me about single cell transcriptomics! 👩‍🔬
• 📫 How to reach me: preferrably through linkedIn