- Fix a bug in abpoa_poa
Download the latest release:
wget https://github.com/yangao07/abPOA/releases/download/v1.2.5/abPOA-v1.2.5.tar.gz
tar -zxvf abPOA-v1.2.5.tar.gz && cd abPOA-v1.2.5
Make from source and run with test data:
make; ./bin/abpoa ./test_data/seq.fa > cons.fa
Or, install via conda and run with test data:
conda install -c bioconda abpoa
abpoa ./test_data/seq.fa > cons.fa
- Introduction
- Installation
- General usage
- Commands and options
- Input
- Output
- For development
- Evaluation datasets
- Contact
abPOA is an extended version of Partial Order Alignment (POA) that performs adaptive banded dynamic programming (DP) with an SIMD implementation. abPOA can perform multiple sequence alignment (MSA) on a set of input sequences and generate a consensus sequence by applying the heaviest bundling algorithm to the final alignment graph.
abPOA can generate high-quality consensus sequences from error-prone long reads and offer significant speed improvement over existing tools.
abPOA supports three alignment modes (global, local, extension) and flexible scoring schemes that allow linear, affine and convex gap penalties. It right now supports SSE2/SSE4.1/AVX2/AVX512F/AVX512BW vectorization and more advanced instructions will be supported in the future.
For more information, please refer to our preprint paper.
On Linux/Unix and Mac OS, abPOA can be installed via
conda install -c bioconda abpoa # install abPOA program
You can also build abPOA from source files. Make sure you have gcc (>=6.4.0) and zlib installed before compiling. It is recommended to download the latest release.
wget https://github.com/yangao07/abPOA/releases/download/v1.2.5/abPOA-v1.2.5.tar.gz
tar -zxvf abPOA-v1.2.5.tar.gz
cd abPOA-v1.2.5; make
Or, you can use git clone command to download the source code.
This gives you the latest version of abPOA, which might be still under development.
git clone https://github.com/yangao07/abPOA.git
cd abPOA; make
If you meet any compiling issue, please try the pre-built binary file:
wget https://github.com/yangao07/abPOA/releases/download/v1.2.5/abPOA-v1.2.5_x64-linux.tar.gz
tar -zxvf abPOA-v1.2.5_x64-linux.tar.gz
abpoa seq.fa > cons.fa
abpoa seq.fa -r2 > cons.out
To generate graph information in GFA format
abpoa seq.fa -r3 > out.gfa
To include the generated consensus sequence as a path in the GFA file:
abpoa seq.fa -r4 > out.gfa
abpoa -i in.gfa seq.fa -r3 > out.gfa
abpoa -i in.msa seq.fa -Ar1 > out.msa
For GFA input file, S and P lines are required and are used to reconstruct the alignment graph.
For MSA input file, which is generally a FASTA format file, - in the sequence indicates the alignment gap.
If you want to use abPOA to generate a MSA output file and then perform the incremental graph alignment, please do not forget -A to include the FASTA header of each sequence:
abpoa seq1.fa -Ar1 > seq1.msa
abpoa -i seq1.msa seq2.fa > cons.fa
abpoa seq.fa -g poa.png > cons.fa
See Plot of alignment graph for more details about the plot file.
Usage: abpoa [options] <in.fa/fq> > cons.fa/msa.out/abpoa.gfa
Options:
Alignment:
-m --aln-mode INT alignment mode [0]
0: global, 1: local, 2: extension
-M --match INT match score [2]
-X --mismatch INT mismatch penalty [4]
-t --matrix FILE scoring matrix file, '-M' and '-X' are not used when '-t' is used [Null]
e.g., 'HOXD70.mtx'
-O --gap-open INT(,INT) gap opening penalty (O1,O2) [4,24]
-E --gap-ext INT(,INT) gap extension penalty (E1,E2) [2,1]
abPOA provides three gap penalty modes, cost of a g-long gap:
- convex (default): min{O1+g*E1, O2+g*E2}
- affine (set O2 as 0): O1+g*E1
- linear (set O1 as 0): g*E1
-s --amb-strand ambiguous strand mode [False]
for each input sequence, try the reverse complement if the current
alignment score is too low, and pick the strand with a higher score
Adaptive banded DP:
-b --extra-b INT first adaptive banding parameter [10]
set b as < 0 to disable adaptive banded DP
-f --extra-f FLOAT second adaptive banding parameter [0.01]
the number of extra bases added on both sites of the band is
b+f*L, where L is the length of the aligned sequence
Minimizer-based seeding and partition (only effective in global alignment mode):
-N --no-seeding disable seeding [False]
-k --k-mer INT minimizer k-mer size [19]
-w --window INT minimizer window size [10]
-n --min-poa-win INT min. size of window to perform POA [50]
-p --progressive build guide tree and perform progressive partial order alignment [False]
Input/Output:
-l --in-list input file is a list of sequence file names [False]
each line is one sequence file containing a set of sequences
which will be aligned by abPOA to generate a consensus sequence
-i --incrmnt FILE incrementally align sequences to an existing graph/MSA [Null]
graph could be in GFA or MSA format generated by abPOA
-o --output FILE ouput to FILE [stdout]
-r --result INT output result mode [0]
- 0: consensus (FASTA format)
- 1: MSA (PIR format)
- 2: both 0 & 1
- 3: graph (GFA format)
- 4: graph with consensus path (GFA format)
-A --msa-header add read ID as header of each sequence in MSA output [False]
-g --out-pog FILE dump final alignment graph to FILE (.pdf/.png) [Null]
-h --help print this help usage information
-v --version show version number
abPOA works with FASTA, FASTQ, gzip'd FASTA(.fa.gz) and gzip'd FASTQ(.fq.gz) formats. The input file is expected to contains multiple sequences which will be processed sequentially to perform the iterative sequence-to-graph (partial order) alignment.
abPOA can also take a list of file names as input with option -l, where each line is the path to one
file containing multiple sequences. Each sequence file is then individually aligned by abPOA to generate a
consensus sequence.
By default, abPOA only outputs the consensus sequence generated from the final alignment agraph. It is in FASTA format with the name field set as "Consensus_sequence". For example:
>Consensus_sequence
ACGTGTACACGTTGAC
abPOA can also output the row-column multiple sequence alignment (RC-MSA) of all the aligned sequences in PIR format
with an additional FASTA header >Multiple_sequence_alignment. For example:
>Multiple_sequence_alignment
ACGTGTACA-GTTGAC
A-G-GTACACGTT-AC
A-GTGT-CACGTTGAC
ACGTGTACA--TTGAC
The - in the sequence stands for alignment gap.
abPOA can output the final alignment graph in GFA format.
Each segment line (S line) represents one node and each link line (L line) represents one edge between two nodes.
The original input sequences and the generated consensus sequence are described as paths in P lines.
abPOA outputs two graph-related numbers in the header line (H line):
NS and NL, which denote the total number of nodes and edges in the GFA file, respectively.
Please refer to the GFA specification for more details of the GFA format.
abPOA can generate a plot of the final partial order alignment graph with the help of graphviz dot.
For example:
The numbers inside the nodes are the node IDs. The numbers on the edges are the edge weights.
S and E are the auxiliary start and end nodes that have no sequence bases.
Make sure you have dot installed beforing using abPOA to generate the plot.
For Linux/Unix systems: sudo apt-get install graphviz.
abPOA is not only a stand-alone tool for MSA and consensus calling, it can also work as a programming library. example.c shows how to use the C APIs of abPOA to take a set of sequences as input and perform MSA and consensus calling. Basically, the library file libabpoa.a and two header files abpoa.h and simd_instruction.h are needed to make the abPOA library work in your program.
abPOA also provides Python bindings to all the primary C APIs. Refer to python/README.md for more details.
The evaluation datasets and scripts used in abPOA paper can be found in abPOA-v1.0.5.
Yan Gao [email protected]
Yi Xing [email protected]
Yadong Wang [email protected]