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Czh3 edited this page Mar 14, 2015 · 8 revisions

#Welcome to the NGSTools wiki!

####With its unprecedented throughput, scalability, and speed, next-generation sequencing enables researchers to study biological systems at a level never before possible. ####Today's complex genomic research questions demand a depth of information beyond the capacity of traditional DNA sequencing technologies. Next-generation sequencing has filled that gap and become an everyday research tool to address these questions.

DATA ANANLYSIS

This is a frame to help you build your NGS pipeline easily.

We are aiming to save your time of leaning using the NGS tools. And help you to build these pipelines:
  • WGS
  • WES
  • RNA-seq
  • Chip-seq
  • RRBS
  • MeDIP

Now, the frame contains several tools that widely used in next generation sequencing data analysis:

  • fastqc
  • cutadapte
  • SAMtools
  • picard
  • Tophat2
  • bowtie2
  • BWA
  • HTSeq
  • GATK
  • DESeq2

In this frame, your does not know much about the tools' parameter, we set the this default by our experience in illumina sequencing platform(Hiseq2000, Hiseq2500, HiseqX).