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diSPIM_screen

Tools to analyse diSPIM imaging data and evaluate mitotic phenotypes

This repository contains scripts used for the analysis of mitotic phenotypes in 3D spheroids based on diSPIM imaging data. The corresponding manuscript is available as a pre-print:

This repository contains:

  • hSPIM image processing pipeline manual (with installation instructions)
  • CNN analysis (in Python) for detecting mitotic phases
  • KNIME workflows and R script for evaluating mitotic phenotypes

To install the hSPIM image processing pipeline, please download the software and tutorial here. This software was developed in collaboration with Markus Fangerau and Ingmar Gergel at mBits imaging GmbH and is free for academic use.

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