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include segment scan on somatic module
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jinzhuangdou authored Aug 23, 2023
1 parent a8be904 commit 9758006
Showing 1 changed file with 3 additions and 3 deletions.
6 changes: 3 additions & 3 deletions README.md
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Expand Up @@ -841,7 +841,7 @@ Users also need to preprare for following files `CCDG_14151_B01_GRM_WGS_2020-08-

```
${path}/src/Monopogen.py germline -a ${path}/apps -r region.lst \
-p ./ \
-p ./ -t 22 \
-g GRCh38.chr20.fa -m 3 -s all -o bm
```
This will take ~ 25 mins with output as
Expand Down Expand Up @@ -1095,7 +1095,7 @@ Then, we need to collect single cell level read information by running the `cell

```
python ${path}/src/Monopogen.py somatic \
-a ${path}/apps -r region.lst -t 50 \
-a ${path}/apps -r region.lst -t 22 -w 10MB \
-i bm -l CB_7K.maester_scRNA.csv -s cellScan \
-g GRCh38.chr20.fa
```
Expand All @@ -1112,7 +1112,7 @@ Lines total/split/realigned/skipped: 1593789/42248/4781/0
Finally, we can run the LD refinment step to further improve the putative somatic SNV detection as (taking ~3 mins)
```
python ${path}/src/Monopogen.py somatic \
-a ${path}/apps -r region.lst -t 50 \
-a ${path}/apps -r region.lst -t 22 \
-i bm -l CB_7K.maester_scRNA.csv -s LDrefinement \
-g GRCh38.chr20.fa
```
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