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# Necrodegradomics
Identification of proteins with pseudo-increased or -decreased abundance after time of tissue degradation. Tissues from kidney, liver and lung were taken from human corpses. Proteins with particular behavior across time were identified using linear models (as implemented in the R packagte `limma`)
# Data analysis and reproducible report for: Retrospective Timing of Tissue Storage by Kinetics of Proteome Alterations – The „Proteomic Clock” Concept

## Kidney proteome
This repository contains the code and reproducible report for the analysis of protein abundance in autopisied tissue and its association with time of degradation.

[Link to report](https://github.com/MiguelCos/Necrodegradomics/blob/main/results/kidney_necrodegreadomics_limma.md)
We hereby share the code and associated visualizations for the identification of proteins with pseudo-increased or -decreased abundance after time of tissue degradation. Tissues from kidney, liver and lung were taken from human corpses. Proteins with particular behavior across time were identified using linear models (as implemented in the R packagte `limma`)

## Liver proteome

[Link to report](https://github.com/MiguelCos/Necrodegradomics/blob/main/results/liver_necrodegreadomics_limma.md)

## Lung proteome

[Link to report](https://github.com/MiguelCos/Necrodegradomics/blob/main/results/lung_necrodegreadomics_limma.md)
## Full reproducible report:

The compiled reproducible report containing the data processing, exploratory, linear model analyses and visualizations can be found in the [link](https://github.com/MiguelCos/Necrodegradomics/blob/main/results/necrodegreadomics_data_analysis_reproducible_report.md).

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