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Opentrons · Aug 14, 2024 · 11a9259 · 11a9259
1 parent c002c44
commit 11a9259
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Showing 3 changed files with 558 additions and 73 deletions.
diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_2_20_8_None_PARTIAL_COLUMN_HappyPath.py b/analyses-snapshot-testing/files/protocols/Flex_S_2_20_8_None_PARTIAL_COLUMN_HappyPath.py
@@ -1,7 +1,7 @@
 from opentrons.protocol_api import PARTIAL_COLUMN
 
 metadata = {
-    "protocolName": "8 Channel SINGLE Happy Path A1 or H1",
+    "protocolName": "8 Channel PARTIAL_COLUMN Happy Path A1 or H1",
     "description": "Tip Rack South Clearance for the 8 Channel pipette and a SINGLE partial tip configuration.",
 }
 
@@ -15,19 +15,21 @@ def run(protocol):
 
     trash = protocol.load_trash_bin("A3")  # must load trash bin
 
+    thermocycler = protocol.load_module("thermocycler module gen2")
+
     partial_tip_rack = protocol.load_labware(
-        load_name="opentrons_flex_96_tiprack_1000ul",
+        load_name="opentrons_flex_96_tiprack_50ul",
         label="Partial Tip Rack",
-        location="C1",
+        location="B3",
     )
 
-    pipette = protocol.load_instrument(instrument_name="flex_8channel_50", mount="left")
+    pipette = protocol.load_instrument(instrument_name="flex_8channel_50", mount="right")
     # mount on the right and you will get an error.
 
     pipette.configure_nozzle_layout(
         style=PARTIAL_COLUMN,
         start="H1",
-        end="G1",  # 2 Tips
+        end="B1",  # 2 Tips
         tip_racks=[partial_tip_rack],
     )
 
@@ -45,6 +47,42 @@ def run(protocol):
 
     volume = 10  # Default volume for actions that require it
 
+    # #############################
+    # # Pipette do work
+    # # leading nozzle
+    # # index in based on eh number of tips in my current configuration
+    # pipette.transfer(volume, source_labware_B2["B6"], destination_labware_C2["A6"])
+
+    # pipette.pick_up_tip()
+    # pipette.touch_tip(source_labware_B2["B1"])
+    # pipette.drop_tip()
+    # pipette.pick_up_tip()
+    # pipette.home()
+    # pipette.drop_tip()
+
+    # pipette.pick_up_tip()
+    # well = source_labware_B2["D1"]
+    # # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
+    # pipette.move_to(well.bottom(z=2))
+    # pipette.mix(10, 10)
+    # pipette.move_to(well.top(z=5))
+    # pipette.blow_out()
+    # pipette.prepare_to_aspirate()
+    # pipette.move_to(well.bottom(z=2))
+    # pipette.aspirate(10, well.bottom(z=2))
+    # pipette.dispense(10)
+    # pipette.drop_tip()
+
+    # ############################
+    # # Change the pipette configuration
+
+    # pipette.configure_nozzle_layout(
+    #     style=PARTIAL_COLUMN,
+    #     start="H1",
+    #     end="B1",  # 7 Tips
+    #     tip_racks=[partial_tip_rack],
+    # )
+
     #############################
     # Pipette do work
 
@@ -69,50 +107,3 @@ def run(protocol):
     pipette.aspirate(10, well.bottom(z=2))
     pipette.dispense(10)
     pipette.drop_tip()
-
-    ############################
-    # Change the pipette configuration
-
-    pipette.configure_nozzle_layout(
-        style=PARTIAL_COLUMN,
-        start="H1",
-        end="B1",  # 7 Tips
-        tip_racks=[partial_tip_rack],
-    )
-
-    source_labware_B3 = protocol.load_labware(
-        load_name="nest_96_wellplate_100ul_pcr_full_skirt",
-        label="B3 Source Labware",
-        location="B3",
-    )
-
-    destination_labware_C3 = protocol.load_labware(
-        load_name="nest_96_wellplate_100ul_pcr_full_skirt",
-        label="C3 Destination Labware",
-        location="C3",
-    )
-
-    #############################
-    # Pipette do work
-
-    pipette.transfer(volume, source_labware_B3["A6"], destination_labware_C3["A6"])
-
-    pipette.pick_up_tip()
-    pipette.touch_tip(source_labware_B3["B1"])
-    pipette.drop_tip()
-    pipette.pick_up_tip()
-    pipette.home()
-    pipette.drop_tip()
-
-    pipette.pick_up_tip()
-    well = source_labware_B3["D1"]
-    # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
-    pipette.move_to(well.bottom(z=2))
-    pipette.mix(10, 10)
-    pipette.move_to(well.top(z=5))
-    pipette.blow_out()
-    pipette.prepare_to_aspirate()
-    pipette.move_to(well.bottom(z=2))
-    pipette.aspirate(10, well.bottom(z=2))
-    pipette.dispense(10)
-    pipette.drop_tip()
diff --git a/analyses-snapshot-testing/files/protocols/Flex_S_2_20_8_None_SINGLE_HappyPath.py b/analyses-snapshot-testing/files/protocols/Flex_S_2_20_8_None_SINGLE_HappyPath.py
@@ -16,12 +16,14 @@ def run(protocol):
     trash = protocol.load_trash_bin("A3")  # must load trash bin
 
     partial_tip_rack = protocol.load_labware(
-        load_name="opentrons_flex_96_tiprack_1000ul",
+        load_name="opentrons_flex_96_tiprack_50ul",
         label="Partial Tip Rack",
-        location="C1",
+        location="B3",
     )
 
-    pipette = protocol.load_instrument(instrument_name="flex_8channel_50", mount="left")
+    thermocycler = protocol.load_module("thermocycler module gen2")
+
+    pipette = protocol.load_instrument(instrument_name="flex_8channel_50", mount="right")
     # mount on the right and you will get an error.
 
     # On the 8-channel SINGLE
@@ -92,43 +94,31 @@ def run(protocol):
         tip_racks=[partial_tip_rack],
     )
 
-    source_labware_B3 = protocol.load_labware(
-        load_name="nest_96_wellplate_100ul_pcr_full_skirt",
-        label="B3 Source Labware",
-        location="B3",
-    )
-
-    destination_labware_C3 = protocol.load_labware(
-        load_name="nest_96_wellplate_100ul_pcr_full_skirt",
-        label="C3 Destination Labware",
-        location="C3",
-    )
-
     #############################
     # Pipette do work
     pipette.consolidate(
         [volume, volume],
-        [source_labware_B3["A3"], source_labware_B3["A4"]],
-        destination_labware_C3["A3"],
+        [source_labware_B2["A3"], source_labware_B2["A4"]],
+        destination_labware_C2["A3"],
     )
 
-    pipette.transfer(volume, source_labware_B3["A6"], destination_labware_C3["A6"])
+    pipette.transfer(volume, source_labware_B2["A6"], destination_labware_C2["A6"])
 
     pipette.distribute(
         5,
         source_labware_B2["A7"],
-        [destination_labware_C3["A7"], destination_labware_C3["A8"]],
+        [destination_labware_C2["A7"], destination_labware_C2["A8"]],
     )
 
     pipette.pick_up_tip()
-    pipette.touch_tip(source_labware_B3["B1"])
+    pipette.touch_tip(source_labware_B2["B1"])
     pipette.drop_tip()
     pipette.pick_up_tip()
     pipette.home()
     pipette.drop_tip()
 
     pipette.pick_up_tip()
-    well = source_labware_B3["D1"]
+    well = source_labware_B2["D1"]
     # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
     pipette.move_to(well.bottom(z=2))
     pipette.mix(10, 10)