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Updated to 1.9.4 (#11)
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RJWANGbioinfo committed Mar 1, 2022
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2 changes: 1 addition & 1 deletion DESCRIPTION
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Package: APAlyzer
Type: Package
Title: A toolkit for APA analysis using RNA-seq data
Version: 1.9.3
Version: 1.9.4
Description: Perform 3'UTR APA, Intronic APA and gene expression analysis using RNA-seq data.
Authors@R: c(person("Ruijia", "Wang", role=c("cre", "aut"),
email="[email protected]",
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2 changes: 2 additions & 0 deletions NEWS
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Expand Up @@ -53,3 +53,5 @@ Changes in version 1.9.2 (2022-01-02)
Changes in version 1.9.3 (2022-02-27)
+ Fixed the issues caused by the lastest ensembl GRCm39 GTF.
+ Fixed typos in vignettes
Changes in version 1.9.4 (2022-02-28)
+ Fixed typos in vignettes
8 changes: 4 additions & 4 deletions README.md
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![Image of logo](https://user-images.githubusercontent.com/51307984/73330398-a9a03880-422e-11ea-9d1b-a1312b47aa1c.png)
# About APAlyzer
[![](https://img.shields.io/badge/release%20version-1.8.0-green.svg)](https://www.bioconductor.org/packages/APAlyzer)
[![](https://img.shields.io/badge/devel%20version-1.9.3-blue.svg)](https://github.com/RJWANGbioinfo/APAlyzer)
[![](https://img.shields.io/badge/devel%20version-1.9.4-blue.svg)](https://github.com/RJWANGbioinfo/APAlyzer)
[![](https://img.shields.io/badge/download-3519/total-blue.svg)](https://bioconductor.org/packages/stats/bioc/APAlyzer)
[![](http://www.bioconductor.org/shields/downloads/release/APAlyzer.svg)](https://bioconductor.org/packages/stats/bioc/APAlyzer)
[![](https://img.shields.io/badge/doi-10.1093/bioinformatics/btaa266-green.svg)](https://doi.org/10.1093/bioinformatics/btaa266)
Expand Down Expand Up @@ -281,11 +281,11 @@ The output contains 4 columns: ‘gene symbol’ describes gene information;
statistical significance based on the Fisher’s exact test; ‘p_adj’ is FDR
adjusted pvalue and ‘APAreg’ is 3’UTR APA regulation pattern in the gene.
We define 3 types in ‘APAreg’, ‘UP’ means aUTR abundance in the treatment group
(‘KD’ in this case) is at least 5% lower than that in control
(‘NT’ in this case), and ‘pvalue’<0.05; ‘DN’ means aUTR abundance is 5% higher
(‘KD’ in this case) is at least 5% higher than that in control
(‘NT’ in this case), and ‘pvalue’<0.05; ‘DN’ means aUTR abundance is 5% lower
in treatment than that in control and p-value<0.05; ‘NC’ are the remaining
genes. With respect to 3’UTR size changes,
‘UP’ means 3’UTR shortening, and ‘DN’ 3’UTR lengthening.
‘UP’ means 3’UTR lengthening, and ‘DN’ 3’UTR shortening.

For the replicate design, we use t-test for significance analysis. However,
other tools based on negative binomial data distribution, such as
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6 changes: 3 additions & 3 deletions vignettes/APAlyzer.Rmd
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Expand Up @@ -311,11 +311,11 @@ The output contains 4 columns: ‘gene symbol’ describes gene information;
statistical significance based on the Fisher’s exact test; ‘p_adj’ is FDR
adjusted pvalue and ‘APAreg’ is 3’UTR APA regulation pattern in the gene.
We define 3 types in ‘APAreg’, ‘UP’ means aUTR abundance in the treatment group
(‘KD’ in this case) is at least 5% lower than that in control
(‘NT’ in this case), and ‘pvalue’<0.05; ‘DN’ means aUTR abundance is 5% higher
(‘KD’ in this case) is at least 5% higher than that in control
(‘NT’ in this case), and ‘pvalue’<0.05; ‘DN’ means aUTR abundance is 5% lower
in treatment than that in control and p-value<0.05; ‘NC’ are the remaining
genes. With respect to 3’UTR size changes,
‘UP’ means 3’UTR shortening, and ‘DN’ 3’UTR lengthening.
‘UP’ means 3’UTR lengthening, and ‘DN’ 3’UTR shortening.

For the replicate design, we use t-test for significance analysis. However,
other tools based on negative binomial data distribution, such as
Expand Down

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