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Preparing input files for pyclone from Varscan output snp files #25

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maheshworpaudel5001 opened this issue Mar 3, 2023 · 0 comments

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@maheshworpaudel5001
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Hello Dear Roth,

Could you help me direct to preparing input files for running pyclone from snp output of VarScan?
Here is the content of a snp file.

Native header | VCF field name | Description

chrom | CHROM | Chromosome or reference name
position | POS | Position from SAMtools pileup (1-based)
ref | REF | Reference base at this position
var | ALT | Variant base seen in tumor
normal_reads1 | RD (col 10) | Reads supporting reference in normal
normal_reads2 | AD (col 10) | Reads supporting variant in normal
normal_var_freq | FREQ (col 10) | Variant allele frequency in normal
normal_gt | GT (col 10) | Consensus genotype call in normal
tumor_reads1 | RD (col 11) | Reads supporting reference in tumor
tumor_reads2 | AD (col 11) | Reads supporting variant in tumor
tumor_var_freq | FREQ (col 11) | Variant allele frequency in tumor
tumor_gt | GT (col 11) | Consensus genotype in tumor
somatic_status | SS (col 8) | Somatic status (Germline, Somatic, LOH, Unknown)
variant_p_value | GPV (col 8) | Variant p-value from FET for germline events (tumor + normal vs reference)
somatic_p_value | SPV (col 8) | Somatic p-value from FET (tumor vs normal)
tumor_reads1_plus | DP4 (col 11) | Tumor reference-supporting reads on + strand
tumor_reads1_minus |   | Tumor reference-supporting reads on – strand
tumor_reads2_plus |   | Tumor variant-supporting reads on + strand
tumor_reads2_minus |   | Tumor variant-supporting reads on - strand
normal_reads1_plus | DP4 (col 10) | Normal reference-supporting reads on + strand
normal_reads1_minus |   | Normal reference-supporting reads on – strand
normal_reads2_plus |   | Normal variant-supporting reads on + strand
normal_reads2_minus |   | Normal variant-supporting reads on - strand

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