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Application of the U-Net convolutional neural network in the segmentation and classification of regions in white blood cells using the Raabin WBC Data dataset.

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U-Net and White Blood Cells

Application of the U-Net convolutional neural network in the segmentation and classification of regions in white blood cells using the Raabin WBC Data dataset.

Using the PyTorch language, the architecture of the original U-Net was developed based on the article "U-Net: Convolutional Networks for Biomedical Image Segmentation" by Olaf Ronneberger et al (2015), according to Figure 1 presented in this article.

Composition of the dataset used:

  • Total of 1145 images;
  • 218 images of basophil cells;
  • 201 images of eosinophil cells;
  • 242 images of lymphocyte cells;
  • 242 images of monocyte cells;
  • 242 images of neutrophil cells.

You can download the dataset from Raabin WBC Data .

Dataset split: 85% for training and 15% for validation (there was no test).

The training environment was on Google Colab, using the free resources available and the possibility of allocation in the GPU.

Training parameters:

  • 30 epochs;
  • Batch size: 5;
  • Learning Rate: 0.0001;
  • Optimizer: optm.Adam, in PyTorch;
  • Loss function: nn.BCEWithLogitsLoss, in PyTorch;
  • Data Augmentation: Rotation, Horizontal Flip, Vertical Flip, and Perspective.

The result of the training with 30 epochs can be observed in the following graph:

As metrics to evaluate the performance of the network, Accuracy and Dice Score were used, reaching 99.34% of Accuracy and 97.16% of Dice Score in the end.

All the resulting images from the training can be accessed in the "results" folder, but here are the results for eosinophil cells:

Hope you enjoy it!

Diego Oliveira

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Application of the U-Net convolutional neural network in the segmentation and classification of regions in white blood cells using the Raabin WBC Data dataset.

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