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This FIJI macros allows quantification of cell death in plants stained with Evans Blue.

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PlantDeath

IT ONLY WORKS WITH FIJI, NOT IMAGEJ

  • This macros allows quantification of cell death in plants stained with Evans Blue.
  • It is based on the transformation from RGB to CIE L*a*b* images. CIELAB source: wikipedia
  • The dead cells are stained in blue while the living cells remains green. It is therfore possible to discimiate them based on theire b* value.
  • The user can chose options: white balance (original macro), whatersheding, scale and minimum area.
  • Examples with Chlamydomonas cells.

Original

original

Threshold

threshold_LAB_b threshold threshold_LAB_b threshold

Continuous

continuous_LAB_b continuous continuous_LAB_b continuous

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This FIJI macros allows quantification of cell death in plants stained with Evans Blue.

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