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Genenotebook 0.4.7 #85

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Oct 10, 2023
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49 changes: 32 additions & 17 deletions tools/genenotebook/genenotebook_build.xml
Original file line number Diff line number Diff line change
Expand Up @@ -40,6 +40,10 @@
genoboo add eggnog @CONNECT_INFO@ '${annot.eggnog}';
#end if

#if $annot.hectar:
genoboo add hectar @CONNECT_INFO@ '${annot.hectar}';
#end if

#if $annot.blast_cond.blast_choice == "blast":
genoboo add blast @CONNECT_INFO@
--format 'xml'
Expand All @@ -57,11 +61,15 @@
#end if

#for exp in $annot.expression:
genoboo add transcriptome @CONNECT_INFO@
--sample-name '${exp.sample_name}'
--replica-group '${exp.replica_group}'
--sample-description '${exp.sample_description}'
'${exp.counts}';
genoboo add expression @CONNECT_INFO@
--sample-description ''
${exp.public}
#for replica in $exp.expression_replicas:
--replicas '${replica.replicas}'
--replica-names '${replica.replica_names}'
#end for
--
'${exp.counts}';
#end for
#end for
#end for
Expand Down Expand Up @@ -97,6 +105,7 @@
</conditional>
<param name="interproscan" label="InterProScan results" optional="true" type="data" format="tsv,gff3" />
<param name="eggnog" label="EggNOG-Mapper results" optional="true" type="data" format="tsv" />
<param name="hectar" label="Hectar results" optional="true" type="data" format="tsv" />

<conditional name="blast_cond">
<param name="blast_choice" type="select" label="Import Blast or Diamond results">
Expand All @@ -120,10 +129,12 @@
</conditional>

<repeat name="expression" title="Expression data">
<param name="counts" label="Expression data" optional="true" type="data" format="tsv" />
<param argument="--sample-name" name="sample_name" label="Unique sample name" type="text" />
<param argument="--replica-group" name="replica_group" label="Identifier to group samples that belong to the same experiment" type="text" />
<param argument="--sample-description" name="sample_description" label="Description of the experiment" type="text" />
<param name="counts" label="Expression data tabular file" help="One line per mRNA, first column is mRNA ID, each other columns is an experimental condition. TPM values preferably." optional="false" type="data" format="tsv" />
<param name="public" label="Public access" type="boolean" truevalue="--public" falsevalue="" value="false" />
<repeat name="expression_replicas" title="Expression replicas">
<param argument="--replica-names" label="Name of a replica group" type="text"/>
<param argument="--replicas" label="Comma-separated list of columns of replicas (e.g. 1,2,3)" type="text" help="Comma-separated list of column positions, which are part of the same replica group. Columns not mentionned in any replica group will be considered to be a single replica experiment." />
</repeat>
</repeat>
</repeat>
</repeat>
Expand Down Expand Up @@ -202,6 +213,7 @@
<param name="annotation" value="annot.gff" />
<param name="interproscan" value="interproscan.tsv" ftype="tsv" />
<param name="eggnog" value="eggnog.tsv" ftype="tsv" />
<param name="hectar" value="hectar.tsv" ftype="tsv" />
</repeat>
</repeat>
<output name="gnb_db" file="output/genome_annot_ips_en.tar.bz2" compare="sim_size" delta="15000" />
Expand Down Expand Up @@ -235,9 +247,7 @@

<repeat name="expression">
<param name="counts" value="exp.tsv" ftype="tsv" />
<param name="sample_name" value="Sample1_rep1" />
<param name="replica_group" value="Sample1" />
<param name="sample_description" value="Pointless experiment" />
<param name="public" value="true" />
</repeat>
</repeat>
</repeat>
Expand All @@ -246,7 +256,7 @@
<has_text text="addGenome succesfully inserted 1 elements" />
<has_text text="addAnnotation succesfully inserted 5 elements" />
<has_text text="addSimilarSequence succesfully inserted 1 elements" />
<has_text text="addTranscriptome succesfully inserted 1 elements" />
<has_text text="addExpression succesfully inserted 4 elements" />
</assert_stdout>
</test>
<test>
Expand All @@ -272,9 +282,14 @@

<repeat name="expression">
<param name="counts" value="exp.tsv" ftype="tsv" />
<param name="sample_name" value="Sample1_rep1" />
<param name="replica_group" value="Sample1" />
<param name="sample_description" value="Pointless experiment" />
<repeat name="expression_replicas">
<param name="replicas" value="1,2" />
<param name="replica_names" value="ReplicaName1" />
</repeat>
<repeat name="expression_replicas">
<param name="replicas" value="3,4" />
<param name="replica_names" value="ReplicaName2" />
</repeat>
</repeat>
</repeat>
</repeat>
Expand All @@ -283,7 +298,7 @@
<has_text text="addGenome succesfully inserted 1 elements" />
<has_text text="addAnnotation succesfully inserted 5 elements" />
<has_text text="addSimilarSequence succesfully inserted 1 elements" />
<has_text text="addTranscriptome succesfully inserted 1 elements" />
<has_text text="addExpression succesfully inserted 4 elements" />
</assert_stdout>
</test>
</tests>
Expand Down
2 changes: 1 addition & 1 deletion tools/genenotebook/macros.xml
Original file line number Diff line number Diff line change
Expand Up @@ -7,7 +7,7 @@
</requirements>
</xml>

<token name="@TOOL_VERSION@">0.4.5</token>
<token name="@TOOL_VERSION@">0.4.7</token>
<token name="@WRAPPER_VERSION@">@TOOL_VERSION@+galaxy0</token>

<xml name="citation">
Expand Down
4 changes: 2 additions & 2 deletions tools/genenotebook/test-data/exp.tsv
Original file line number Diff line number Diff line change
@@ -1,2 +1,2 @@
target_id length eff_length est_counts tpm
MMUCEDO_000001-T1 1221 1021.99 21 1.80368
gene Sample1 Sample2 Sample3 Sample4
MMUCEDO_000001-T1 1000 2000 3000 4000
2 changes: 2 additions & 0 deletions tools/genenotebook/test-data/hectar.tsv
Original file line number Diff line number Diff line change
@@ -0,0 +1,2 @@
protein id predicted targeting category signal peptide score signal peptide cleavage site type II signal anchor score chloroplast score mitochondrion score other score
MMUCEDO_000002-T1 other localisation 0.0583 - 0.0228 - 0.1032 0.8968